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Glatiramer acetate elevates cell production in the mature retinal pigment epithelium

Published online by Cambridge University Press:  22 December 2011

GOLNAZ SHAHABI  and
GLEN JEFFERY
GOLNAZ SHAHABI
Affiliation:
Institute of Ophthalmology, University College London, London, UK
GLEN JEFFERY*
Affiliation:
Institute of Ophthalmology, University College London, London, UK
*
*Address correspondence and reprint requests to: Glen Jeffery, Institute of Ophthalmology, University College London, 11-43 Bath Street, London EC1V 9EL, UK. E-mail: [email protected]
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Abstract

The retinal-pigmented epithelium (RPE) is critical for visual function. Throughout life, central RPE cells are lost but replenished by peripheral cell production. Glatiramer acetate increases neuronal production in mature brains and is thought to erode age-related deposits in the human retina that are risk factors for macular degeneration. Here, we ask whether this agent also elevates RPE production in mature rat eyes. If so, it may be used to replenish these cells in damaged eyes. Glatiramer acetate was given systemically for 14 days combined with Bromodeoxyuridine (BrdU) to mark cell division. One eye was then processed for the cell cycle marker Ki67 and the other for BrdU. Glatiramer acetate significantly elevated the number of RPE cells in the cell cycle, with more labeled with Ki67. There were also significantly more BrdU-labeled cells over the 14 days, confirming that some cells divided. However, while Ki67 positive cell numbers increased by approximately 100% following examination at one time point, BrdU cell numbers increased by only 3% when averaged per day. Hence, glatiramer acetate induces cells to proliferate, but many may fail either to complete division or to survive. This may have long-term consequences for this tissue.

Keywords

RPEProliferationCell cycleKi67BromodeoxyduridineAge-related macular degeneration
Type
Brief Communication
Information
Visual Neuroscience , Volume 28 , Issue 6 , November 2011 , pp. 543 - 546
Copyright
Copyright © Cambridge University Press 2011

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