Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-23T08:31:54.745Z Has data issue: false hasContentIssue false
  • English
  • Français

The development of MK-801, kainate, AMPA, and muscimol binding sites in cat visual cortex

Published online by Cambridge University Press:  02 June 2009

Barbara Gordon ,
Ying L. Tseng ,
Rose Jaeger ,
Alexandra Petrovic  and
Kenneth Tovar
Barbara Gordon
Affiliation:
Institute of Neuroscience, University of Oregon, Eugene
Ying L. Tseng
Affiliation:
Institute of Neuroscience, University of Oregon, Eugene
Rose Jaeger
Affiliation:
Institute of Neuroscience, University of Oregon, Eugene
Alexandra Petrovic
Affiliation:
Institute of Neuroscience, University of Oregon, Eugene
Kenneth Tovar
Affiliation:
Institute of Neuroscience, University of Oregon, Eugene
Get access Rights & Permissions [Opens in a new window]

Abstract

Previous work using homogenate binding has shown that the development of (+)-5-methyl-10, ll-dihydro-5H-dibenzo[a, d]-cyclohepten-5, 10imine maleate (MK-801) binding in cat visual cortex increases from 21 days to 42 days, the height of the plastic period, and decreases in adulthood. We have studied the generality of this finding by examining the development of NMDA binding sites in several brain regions and by examining the development of other binding sites in the visual cortex. After confirming the original finding, we extended it by showing that the sensitivity of MK-801 binding sites to glutamate and glycine decreases when the cat becomes an adult. We then examined the regional specificity of MK-801 binding. Retinal binding did not change significantly with age. Binding in both visual cortex and hippocampus increased significantly from 7 days to 42 days regardless of whether binding was measured per milligram wet weight or per milligram protein. The decline from 42 days to adulthood was less dramatic in the hippocampus than in the visual cortex and was statistically significant only when binding was measured per milligram protein. Saturation analyses also showed a difference in the two structures. Bmax in the visual cortex, but not in the hippocampus, decreased from 42 days to adulthood. To determine whether these developmental changes were specific to MK-801 binding sites, we compared the age-dependent binding of MK-801, kainate, alpha-amino-3-hydroxy-5-methyl-4-isoxazoIepropionic acid (AMPA), and muscimol. Like MK-801, kainate binding increased from 7 days to 42 days and decreased from 42 days to adulthood. AMPA and muscimol binding showed a similar increase in binding from 7 days to 42 days but did not decrease significantly from 42 days to adulthood. Displacement experiments suggest that AMPA and kainate bind to separate sites. The 42-day peak in NMDA and kainate binding suggests that their associated receptors may have a role in determining the plastic period of visual cortex.

Keywords

Visual cortexGlutamate binding sitesMK-801 binding sitesKainate binding sitesAMPA binding sitesMuscimol binding sites
Type
Research Articles
Information
Visual Neuroscience , Volume 12 , Issue 2 , March 1995 , pp. 241 - 252
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Araneda, R.C., Zukin, R.S. & Bennett, M.V. (1993). Effects of polyamines on NMDA-induced currents in rat hippocampal neurons: A whole-cell and single-channel study. Neuroscience Letters 152, 107–112.CrossRefGoogle ScholarPubMed
Aroniadou, V.A. & Teyler, T.J. (1992). Induction of NMDA receptor-independent long-term potentiation (LTP) in visual cortex of adult rats. Brain Research 584, 169–173.CrossRefGoogle ScholarPubMed
Artola, A. & Singer, W. (1987). Long-term potentiation and NMDA receptors in rat visual cortex. Nature (London) 330, 649–653.CrossRefGoogle ScholarPubMed
Ascher, P. & Johnson, J.W. (1989). The NMDA receptor, its channel and its modulation by glycine. In The NMDA Receptor, ed. Watkins, J.C. & Collingridge, G.L., pp. 109–122. New York: Oxford University Press.Google Scholar
Bear, M.F. & Kirkwood, A. (1993). Neocortical long-term potentiation. Current Opinion in Neurobiology 3, 197–202.CrossRefGoogle ScholarPubMed
Bear, M.F., Kleinschmidt, A. & Singer, W. (1990). Disruption of experience-dependent synaptic modifications in striate cortex by infusion of an NMDA receptor antagonist. Journal of Neuroscience 10, 909–925.CrossRefGoogle ScholarPubMed
Bear, M.F., Press, W.A. & Connors, B.W. (1992). Long-term potentiation in slices of kitten visual cortex and the effects of NMDA receptor blockade. Journal of Neurophysiology 67, 841–851.CrossRefGoogle ScholarPubMed
Berry, R.L., Teyler, T.J. & Han, T.Z. (1989). Induction of LTP in rat primary visual cortex: Tetanus parameters. Brain Research 481, 221–227.CrossRefGoogle ScholarPubMed
Boulter, J., Hollmann, M., O'Shea-Greenfield, A., Hartley, M., Deneris, E., Maron, C. & Heinemann, S. (1990). Molecular cloning and functional expression of glutamate receptor subunit genes. Science 249, 1003–1007.CrossRefGoogle ScholarPubMed
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Carmignoto, G. & Vicini, S. (1992). Activity-dependent decrease in NMDA receptor responses during development of the visual cortex. Science 258, 1007–1011.CrossRefGoogle ScholarPubMed
Cleland, B.G., Mitchell, D.E., Gillard Crewther, S. & Crewther, D.P. (1980). Visual resolution of retinal ganglion cells in monocularly deprived cats. Brain Research 192, 261–266.CrossRefGoogle ScholarPubMed
Collingridge, G.L. & Bliss, T.V.P. (1987). NMDA receptors—their role in long-term potentiation. Trends in Neuroscience 10, 288–293.CrossRefGoogle Scholar
Cotman, C.W. & Iversen, L.L. (1987). Excitatory amino acids in the brain-focus on NMDA receptors. Trends in Neuroscience 10, 263–265.CrossRefGoogle Scholar
Forsythe, I.D., Westbrook, G.L. & Mayer, M.L. (1988). Modulation of excitatory synaptic transmission by glycine and zinc in cultures of mouse hippocampal neurons. Journal of Neuroscience 8, 3733–3741.CrossRefGoogle ScholarPubMed
Foster, A.C. & Wong, E.F.H. (1987). The novel anticonvulsant MK-801 binds to the activated state of the N-methyl-D-aspartate receptor. British Journal of Pharmacology 1991, 403–409.CrossRefGoogle Scholar
Fox, K. & Daw, N.D. (1993). Do NMDA receptors have a critical role in visual cortex plasticity? Trends in Neuroscience 16, 116–122.CrossRefGoogle Scholar
Fox, K., Sato, H. & Daw, N. (1989). The location and function of NMDA receptors in cat and kitten visual cortex. Journal of Neuroscience 9, 2443–2454.CrossRefGoogle Scholar
Gordon, B., Daw, N. & Parkinson, D. (1991). The effect of age on binding of MK-801 in the cat visual cortex. Developmental Brain Research 62, 61–67.CrossRefGoogle ScholarPubMed
Huettner, J.E. & Bean, B.P. (1988). Block of N-methyl-D-aspartate activated current by the anticonvulsant MK-801: Selective binding to open channels. Proceedings of the National Academy of Sciences of the U.S.A. 85, 1307–1311.CrossRefGoogle ScholarPubMed
Johnson, J.W. & Ascher, P. (1992). Equilibrium and kinetic study of glycine action on the N-methyl-D-aspartate receptor in cultured mouse brain neurons. Journal of Physiology (London) 455, 339–365.CrossRefGoogle ScholarPubMed
Kimura, F., Nishigori, A., Shirokawa, T. & Tsumoto, T. (1989). Long-term potentiation and N-methyl-D-aspartate receptors in the visual cortex of young rats. Journal of Physiology 414, 125–144.CrossRefGoogle ScholarPubMed
Kleckner, N.W. & Dingledine, R. (1988). Requirement for glycine in activation of NMDA-receptors expressed in Xenopus oocytes. Science 241, 835–837.CrossRefGoogle ScholarPubMed
Kleinschmidt, A., Bear, M.F. & Singer, W. (1987). Blockage of “NMDA” receptors disrupts experience-dependent plasticity of kitten striate cortex. Science 238, 355–358.CrossRefGoogle Scholar
Leumann, J., Colpaert, F. & Canton, H. (1991). Glutamate and glycine co-activate while polyamines merely modulate the NMDA receptor complex. Progress in Neuropsychopharmacology and Biological Psychiatry 15, 183–190.CrossRefGoogle Scholar
Lerma, J., Paternain, A.V., Naranjoo, J.R. & Mellstrom, B. (1993). Functional kainate selective glutamate receptors in hippocampal cells. Society for Neuroscience Abstracts 19, 624.Google Scholar
Lodge, D., Jones, M. & Fletcher, E. (1989). Non-competitive antagonists of N-methyl-D-aspartate In The NMDA Receptor, ed. Watkins, J.C. & Collingridge, G.L., pp. 37–52. Oxford: Oxford University Press.Google Scholar
McGurk, J.F., Bennett, M.V. & Zukin, R.S. (1990). Polyamines potentiate responses of N-methyl-D-aspartate receptors expressed in xenopus oocytes. Proceedings of the National Acadamy of Sciences of the U.S.A. 87, 9971–9974.CrossRefGoogle ScholarPubMed
Miller, K.D., Chapman, B. & Stryker, M.P. (1989). Visual responses in adult cat visual cortex depend on N-methyl-D-Aspartate receptors. Proceedings of the National Academy of Sciences of the U.S.A. 86, 5183–5187.CrossRefGoogle ScholarPubMed
Murphy, D.E., Snowhill, E.W. & Williams, M. (1987). Characterization of quisqualate recognition sites in rat brain tissue using DL-[3H]alpha-amino-3-hydroxy-5-methylisoxazole-4-propionicacid (AMPA) and a filtration assay. Neurochemical Research 12, 775–782.CrossRefGoogle Scholar
Nakanishi, S. (1992). Molecular diversity of glutamate receptors and implications for brain function. Science 258, 598–603.CrossRefGoogle ScholarPubMed
Olson, C.R. & Freeman, R.D. (1980). Profile of the sensitive period for monocular deprivation in kittens. Experimental Brain Research 39, 17–21.CrossRefGoogle ScholarPubMed
Rauschecker, J.P., Egert, U. & Kossell, H. (1990). Effects of NMDA antagonists on developmental plasticity in kitten visual cortex. International Journal of Developmental Neuroscience 8, 425–435.CrossRefGoogle ScholarPubMed
Reiter, H.O., Waltzman, D.M. & Stryker, M.P. (1986). Cortical activity blockade prevents ocular dominance plasticity in the kitten visual cortex. Experimental Brain Research 65, 182–188.CrossRefGoogle ScholarPubMed
Reynolds, I.J. & Bear, M.F. (1991). Effects of age and visual experience on [3H]MK801 binding to NMDA receptors in the kitten visual cortex. Experimental Brain Research 85, 611–615.CrossRefGoogle ScholarPubMed
Reynolds, I.J., Murphy, S.H. & Miller, R.J. (1987). 3H-labeled MK-801 binding to the excitatory amino acid receptor complex from rat brain is enhanced by glycine. Proceedings of the National Academy of Sciences of the U.S.A. 84, 7744–7748.CrossRefGoogle Scholar
Sheng, M., Cummings, J., Roldan, L.A., Jan, Y.N. & Jan, L.Y. (1994). Changing subunit composition of heteromeric NMDA receptors during development of rat cortex. Nature 368, 144–147.CrossRefGoogle ScholarPubMed
Spear, P.D. & Hou, V. (1990). Retinal ganglion-cell densities and soma sizes are unaffected by long-term monocular deprivation in the cat. Brain Research 522, 354–358.CrossRefGoogle ScholarPubMed
Stryker, M.P. & Harris, W.A. (1986). Binocular impulse blockade prevents the formation of ocular dominance columns in cat visual cortex. Journal of Neuroscience 6, 2117–2133.CrossRefGoogle ScholarPubMed
Teyler, T.J. (1989). Comparative aspects of hippocampal and neocor-tical long-term potentiation. Journal of Neuroscience Methods 28, 101–108.CrossRefGoogle ScholarPubMed
Teyler, T.J., Perkins, A.T. IV, & Harris, K.M. (1989). The development of long-term potentiation in hippocampus and neocortex. Neuropsychologia 27, 31–39.CrossRefGoogle ScholarPubMed
Wiesel, T.N. & Hubel, D.H. (1963). Single cell responses in striate cortex of kittens deprived of vision in one eye. Journal of Neurophysi-ology 26, 1003–1007.CrossRefGoogle ScholarPubMed