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Cycle GMP-activated channels of rod photoreceptors show neither fast nor slow desensitization

Published online by Cambridge University Press:  02 June 2009

Shu-Ichi Watanabe  and
Gary Matthews
Shu-Ichi Watanabe
Affiliation:
Department of Neurobiology and Behavior, The State University of New York, Stony Brook
Gary Matthews
Affiliation:
Department of Neurobiology and Behavior, The State University of New York, Stony Brook
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Abstract

Desensitization of cGMP-activated channels was examined in excised, inside-out patches obtained from rod photoreceptors. Cyclic GMP was applied using a rapid-flow system in which concentration jumps are complete within 10–50 ms. In outer-segment patches containing many channels, the cGMP-dependent conductance reached a steady plateau that was maintained for tens of seconds in the presence of cGMP; thus, there was no indication of slow desensitization. However, rapid desensitization on the scale of milliseconds could not be ruled out because of limited speed of access of cGMP to the inner face of the patch membrane. To test for rapid desensitization, inner-segment patches containing only a single cGMP-activated channel were used. In these one-channel patches, there was no change in activity of the channel with time from its earliest onset after application of cGMP, indicating that rapid desensitization also did not occur.

Keywords

Ion channelsPhotoreceptorsCyclic GMPDesensitizationPhototransduction
Type
Research Articles
Information
Visual Neuroscience , Volume 4 , Issue 5 , May 1990 , pp. 481 - 487
Copyright
Copyright © Cambridge University Press 1990

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