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Correlation between a bicuculline-resistant response to GABA and GABAA receptor ρ1 subunit expression in single rat retinal bipolar cells

Published online by Cambridge University Press:  02 June 2009

Hermes H. Yeh ,
Elena V. Grigorenko  and
Margaret L. Veruki
Hermes H. Yeh
Affiliation:
Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Winston-Salem
Elena V. Grigorenko
Affiliation:
Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Winston-Salem
Margaret L. Veruki
Affiliation:
Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Winston-Salem
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Abstract

Using patch-clamp recording in combination with reverse transcriptase-polymerase chain reaction (RT-PCR), we show in individual bipolar cells acutely dissociated from the adult rat retina a correlation between the expression of the GABAA receptor ρ1 subunit mRNA and a bicuculline-resistant, diazepam-insensitive component of the GABA-activated whole-cell current response. This “GABAC-like” response, contributing to approximately 42% of the GABA-activated whole-cell current and displaying variable sensitivity to picrotoxin, was found in bipolar cells but not in any of the ganglion cells examined. Expression profiling of GABAA receptor subunit mRNAs in individual electrophysiologically tested retinal neurons revealed that, while both bipolar cells and ganglion cells may express numerous GABAA receptor subunit isoforms, including that of ρ2, the expression of the ρ1 subunit was strictly limited to bipolar cells. We propose a possible link between the presence of a receptor with GABAC-like pharmacological profile and the expression of the retina-specific ρ1 subunit isoform. The results presented in this study constitute the first direct demonstration of such a correlation at the single-cell level.

Keywords

Rat retinaBipolar cellGanglion cellBicuculline-resistant GABA responseGABAA receptor ρ subunitCombined patch clamp/single-cell RT-PCR
Type
Research Articles
Information
Visual Neuroscience , Volume 13 , Issue 2 , March 1996 , pp. 283 - 292
Copyright
Copyright © Cambridge University Press 1996

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