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A comparison of GABAC and ρ subunit receptors from the white perch retina

Published online by Cambridge University Press:  02 June 2009

Aohua Qian ,
George Hyatt ,
Andres Schanzer ,
Rohan Hazra ,
Abigail S. Hackam ,
Garry R. Cutting  and
John E. Dowling
Aohua Qian
Affiliation:
Department of Molecular and Cellular Biology, Harvard University, Cambridge
George Hyatt
Affiliation:
Department of Molecular and Cellular Biology, Harvard University, Cambridge
Andres Schanzer
Affiliation:
Department of Molecular and Cellular Biology, Harvard University, Cambridge
Rohan Hazra
Affiliation:
Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore
Abigail S. Hackam
Affiliation:
Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore
Garry R. Cutting
Affiliation:
Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore
John E. Dowling
Affiliation:
Department of Molecular and Cellular Biology, Harvard University, Cambridge
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Abstract

There is increasing evidence that GABAC receptors are composed of GABA ρ subunits. In this study, we compared the properties of native GABAC receptors with those of receptors composed of a GABA ρ subunit. A homologue of the GABA ρ gene was cloned from a white perch (Roccus americana) retinal cDNA library. The clone (perch-s) has an open reading frame of 1422 nucleotide base pairs and encodes a predicted protein of 473 amino acids. It is highly homologous to GABA ρ subunits cloned from human and rat retinas. The receptors (perch-s receptor) expressed by this gene in Xenopus oocytes show properties similar to those of the GABAC receptors present on white perch retinal neurons. GABA induced a sustained response that had a reversal potential of –27.1 +minus; 3.6 mV. The EC50 for the response was 1.74 +− 1.25 μM, a value similar to that reported for GABAC receptors. Pharmacologically, the responses were bicuculline insensitive and not modulated by either diazepam or pentobarbital as is the case for GABAc receptors. There were, however, some distinct differences between native GABAc and perch-s receptors. I4AA acts as a partial agonist on perch-s receptors whereas it is strictly an antagonist on native GABAC receptors. Picrotoxin inhibition is noncompetitive on perch-s receptors, but both competitive and noncompetitive on GABAC receptors. We conclude that GABAC receptors are formed by GABA p subunits but that native GABAc receptors probably consist of a mixture of GABA ρ subunits.

Keywords

GABAC receptorsGABA ρ subunitsWhite perchRetinaGABALigand-gated receptorsHorizontal cells
Type
Research Articles
Information
Visual Neuroscience , Volume 14 , Issue 5 , September 1997 , pp. 843 - 851
Copyright
Copyright © Cambridge University Press 1997

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References

Albrecht, B.E. & Darlison, M.G. (1995). Localization of the ρ-1 and ρ-2 subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of gamma-aminobutyric acid type C receptor subtypes. Neuroscience Letters 189, 155–158.CrossRefGoogle ScholarPubMed
Amin, J. & Weiss, D.S. (1994). Homomeric ρl GABA channels: Activation properties and domains. Receptors and Channels 2, 227–236.Google Scholar
Chang, Y, Amin, J. & Weiss, D.S. (1995). Zinc is a mixed antagonist of homomeric ρ1 γ-aminobutyric acid-activated channels. Molecular Pharmacology 47, 595–602.Google Scholar
Chappell, R.L., Qian, H., Malchow, R.P. & Ripps, H. (1995). Novel action of zinc on GABA receptors of skate bipolar cells. Investigative Ophthalmology and Visual Science (Suppl.), 36, s215.Google Scholar
Cutting, G.R., Lu, L., O'Hara, B.F., Kasch, L.M., Montrose-Rafizadeh, C, Donovan, D.M., Shimada, S, Antonarakis, S.E., Guggino, W.B., Uhl, G.R. & Kazazian, H.H. (1991). Cloning of the γ-aminobutyric acid (GABA) ρ1 cDNA: A GABA subunit highly expressed in the retina. Proceedings of the National Academy of Sciences of the U.S.A. 88, 2673–2677.CrossRefGoogle Scholar
Cutting, G.R., Curristin, S., Zoghbi, H., O'Hara, B., Seldin, M.F. & Uhl, G.R. (1992). Identification of a putative GABA ρ2 receptor sub-unit cDNA and colocalization of the genes encoding ρ2 and ρ1 to human chromosome 6q14-q21 and mouse chromosome 4. Genomics 12, 801–806.CrossRefGoogle Scholar
Dong, C.-J. & Werblin, F.S. (1994). Dopamine modulation of GABAc receptor function in an isolated retinal neuron. Journal of Neurophysiology 71, 1258–1260.CrossRefGoogle Scholar
Dong, C.J. & Werblin, F.S. (1995). Zinc downmodulates the GABAC receptor current in cone horizontal cell acutely isolated from the catfish retina. Journal of Neurophysiology 73, 916–919.CrossRefGoogle ScholarPubMed
Dong, C.-J., Picaud, S.A. & Werblin, F.S. (1994). GABA transporters and GABAc-like receptors on catfish cone- but not rod-driven horizontal cells. Journal of Neuroscience 14, 2648–2658.CrossRefGoogle Scholar
Enz, R., Brandstatter, J.H., Hartveit, E., Wassle, H. & Bormann, J. (1995). Expression of GABA receptor ρ-1 and ρ-2 subunits in the retina and brain of the rat. European Journal of Neuroscience 7, 1495–1501.CrossRefGoogle ScholarPubMed
Feigenspan, A. & Bormann, J. (1994). Modulation of GABAC receptors in rat retinal bipolar cells by protein kinase C. Journal of Physiology (London) 481, 325–330.CrossRefGoogle ScholarPubMed
Feigenspan, A., Wassle, H. & Bormann, J. (1993). Pharmacology of GABA receptor C1− channels in rat retinal bipolar cells. Nature 361, 159–162.CrossRefGoogle Scholar
Grant, G.B. & Werblin, F.S. (1994). Low-cost data acquisition and analysis programs for electrophysiology. Journal of Neuroscience Methods 55, 89–98.CrossRefGoogle ScholarPubMed
Hamosh, A., Trapnell, B.C., Zeitlin, P.L., Montrose-Mafizadeh, C, Rosenstein, B.J., Crystal, R.G. & Cutting, G.R. (1991). Severe deficiency of cystic fibrosis transmembrane conductance regulator messenger RNA carrying nonsense mutations R553X and W1316X in respiratory epithelial cells of patients with cystic fibrosis. Journal of Clinical Investigation 88, 1880–1885.CrossRefGoogle ScholarPubMed
Kusano, K., Miledi, R. & Stinnakre, J. (1982). Cholinergic and cat-echolaminergic receptors in the Xenopus oocyte membrane. Journal of Physiology (London) 328, 143–170.CrossRefGoogle ScholarPubMed
Lukasiewicz, P.D., Maple, B.R. & Werblin, F.S. (1994). A novel GABA receptor on bipolar cell terminal in the tiger salamander retina. Journal of Neuroscience 14, 1202–1212.CrossRefGoogle ScholarPubMed
Ogurusu, T. & Shingai, R. (1996). Cloning of a putative gamma-aminobutyric acid (GABA) receptor subunit rho 3 cDNA. Biochimica et Biophysica Acta 1305, 15–18.CrossRefGoogle ScholarPubMed
Ogurusu, T, Taira, H. & Shingai, R. (1995). Identification of GABAA receptor subunits in rat retina: Cloning of the rat GABAA receptor rho 2-subunit cDNA. Journal of Neurochemistry 65, 964–968.CrossRefGoogle ScholarPubMed
Pan, Z.-H. & Lipton, S.A. (1995). Multiple GABA receptor subtypes mediate inhibition of calcium influx at rat retinal bipolar cell terminals. Journal of Neuroscience 15, 2668–2679.CrossRefGoogle ScholarPubMed
Qian, H. & Dowling, J.E. (1993). Novel GABA responses from rod-driven retinal horizontal cells. Nature 361, 162–164.CrossRefGoogle ScholarPubMed
Qian, H. & Dowling, J.E. (1994). Pharmacology of novel GABA receptors found on rod horizontal cells of the white perch retina. Journal of Neuroscience 14, 4299–4307.CrossRefGoogle ScholarPubMed
Qian, H. & Dowling, J.E. (1995). GABAA and GABAC receptors on hybrid bass retinal bipolar cells. Journal of Neurophysiology 74, 1920–1928.CrossRefGoogle ScholarPubMed
Robinson, J., Schmitt, E.A., Harosi, E, Reece, R. & Dowling, J.E. (1993). Zebrafish ultraviolet visual pigment: Absorption spectrum, sequence, and localization. Proceedings of the National Academy of Sciences of the U.S.A. 90, 6009–6012.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Shimada, S., Cutting, G. & Uhl, G.R. (1992). γ-aminobutyric acid A or C receptor?-aminobutyric acid ρ1 receptor RNA induces bicuculline-, barbiturate-, and benzodiazepine-insensitive γ-aminobutyric acid responses in Xenopus oocytes. Molecular Pharmacology 41, 683–687.Google ScholarPubMed
Strata, F. & Cherubini, E. (1994). Transient expression of a novel type of GABA response in rat CA3 hippocampal neurons during development. Journal of Physiology (London) 480, 493–503.CrossRefGoogle ScholarPubMed
Takahashi, K.I., Miyoshi, S. & Kaneko, A. (1995). GABA-induced chloride current in catfish horizontal cells mediated by non-GABAA receptor channels. Japanese Journal of Physiology 45, 437–456.Google ScholarPubMed
Wang, T.L., Guggino, W.B. & Cutting, G.R. (1994). A novel γ-aminobutyric acid receptor subunit (ρ2) cloned from human retina forms bicuculline-insensitive homooligomeric receptors in Xenopus oocytes. Journal of Neuroscience 14, 6524–6531.CrossRefGoogle Scholar
Wang, T.L., Hackam, A.S., Guggino, W.B. & Cutting, G.R. (1995 b). A single histidine residue is essential for zinc inhibition of GABA ρ-1 receptors. Journal of Neuroscience 15, 7684–7691.CrossRefGoogle ScholarPubMed
Wang, T.L., Hackam, A.S., Guggino, W.B. & Cutting, G.R. (1995 b). A single amino acid in γ-aminobutyric acid ρ1 receptors affects competitive and noncompetitive components of picrotoxin inhibition. Proceedings of the National Academy of Sciences of the U.S.A. 92, 11751–11755.CrossRefGoogle Scholar
Wellis, D.P. & Werblin, F.S. (1995). Dopamine modulates GABAC receptors mediating inhibition of calcium entry into and transmitter release from bipolar cell terminals in tiger salamander retina. Journal of Neuroscience 15, 4748–4761.CrossRefGoogle ScholarPubMed
Zhang, D., Pan, Z.-H., Zhang, X., Brideau, A.D. & Lipton, S.A. (1995). Cloning of aγ-aminobutyric acid type C receptor subunit in rat retina with a methionine residue critical for picrotoxinin channel block. Proceedings of the National Academy of Sciences of the U.S.A. 92, 11756–11760.CrossRefGoogle Scholar