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Test of the paired-flash electroretinographic method in mice lacking b-waves

Published online by Cambridge University Press:  19 July 2007

JENNIFER J. KANG DERWENT ,
SHANNON M. SASZIK ,
HIDETAKA MAEDA ,
DEBORAH M. LITTLE ,
MACHELLE T. PARDUE ,
LAURA J. FRISHMAN  and
DAVID R. PEPPERBERG
JENNIFER J. KANG DERWENT
Affiliation:
Lions of Illinois Eye Research Institute, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, College of Medicine, Chicago, Illinois Department of Biomedical Engineering, Pritzker Institute of Biomedical Science and Engineering, Illinois Institute of Technology, Chicago, Illinois
SHANNON M. SASZIK
Affiliation:
College of Optometry, University of Houston, Houston, Texas Department of Ophthalmology, Northwestern University School of Medicine, Chicago, Illinois
HIDETAKA MAEDA
Affiliation:
College of Optometry, University of Houston, Houston, Texas Kobe University Medical School, Kobe, Japan
DEBORAH M. LITTLE
Affiliation:
Department of Neurology and Rehabilitation, and Center for Cognitive Medicine, University of Illinois at Chicago, College of Medicine, Chicago, Illinois
MACHELLE T. PARDUE
Affiliation:
Atlanta VA Medical Center, Decatur, Georgia Department of Ophthalmology, Emory University, Atlanta, Georgia
LAURA J. FRISHMAN
Affiliation:
College of Optometry, University of Houston, Houston, Texas
DAVID R. PEPPERBERG
Affiliation:
Lions of Illinois Eye Research Institute, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, College of Medicine, Chicago, Illinois
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Abstract

Previous studies of rod photoreceptors in vivo have employed a paired-flash electroretinographic (ERG) technique to determine rod response properties. To test whether absence versus presence of the ERG b-wave affects the photoreceptor response derived by the paired-flash method, we examined paired-flash-derived responses obtained from nob mice, a mutant strain with a defect in signal transduction between photoreceptors and ON bipolar cells that causes a lack of the b-wave. Normal littermates of the nob mice served as controls. The normalized amplitude-intensity relation of the derived response determined in nob mice at the near-peak time of 86 ms was similar to that determined for the controls. The full time course of the derived rod response was obtained for test flash strengths ranging from 0.11 to 17.38 scotopic cd s m−2 (sc cd s m−2). Time-course data obtained from nob and control mice exhibited significant but generally modest differences. With saturating test flash strengths, half-recovery times for the derived response of nobversus control mice differed by ∼60 ms or less about the combined (nob and control) average respective values. Time course data also were obtained before versus after intravitreal injection of l-2-amino-4-phosphonobutyrate (APB) (which blocks transmission from photoreceptors to depolarizing bipolar cells) and of cis 2,3-piperidine dicarboxylic acid (PDA) (which blocks transmission to OFF bipolar cells, and to horizontal, amacrine and ganglion cells). Neither APB nor PDA substantially affected derived responses obtained from nob or control mice. The results provide quantitative information on the effect of b-wave removal on the paired-flash-derived response in mouse. They argue against a substantial skewing effect of the b-wave on the paired-flash-derived response obtained in normal mice and are consistent with the notion that, to good approximation, this derived response represents the isolated flash response of the photoreceptors in both nob and normal mice.

Keywords

Rod photoreceptorElectroretinogramPaired-flash ERGnob mouse
Type
Research Article
Information
Visual Neuroscience , Volume 24 , Issue 2 , March 2007 , pp. 141 - 149
Copyright
2007 Cambridge University Press

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