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Regenerating axons from adult chick retinal ganglion cells recognize topographic cues from embryonic central targets

Published online by Cambridge University Press:  02 June 2009

Jens Vanselow ,
Bernhard Müller  and
Solon Thanos
Jens Vanselow
Affiliation:
Max-Planck-Institute for Developmental Biology, Tübingen, Germany
Bernhard Müller
Affiliation:
Max-Planck-Institute for Developmental Biology, Tübingen, Germany
Solon Thanos
Affiliation:
Department of Ophthalmology, University of Tübingen, School of Medicine, Tübingen, Germany
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Abstract

We investigated whether regenerating mature axons recapitulate embryonic features essential to successful reconnectivity within the injured nervous system. Strips from embryonic and adult chick retinae were cultured, and outgrowing axons were examined morphometrically and immunohistochemically. In addition, the target-recognition properties of adult neurites were analyzed. Regenerating adult axons elongate on a poly-L-lysine/laminin substratum with a speed about one order of magnitude slower than that of embryonic axons. Morphologically, adult axonal tips differ dramatically from embryonic growth cones in that they possess only filopodial extensions whereas embryonic growth cones possess both lamellipodial and filopodial processes. Both embryonic and adult neurites express the growth-associated protein GAP-43. When cultured on alternating stripes of anterior and posterior embryonic tectal membranes, both adult and embryonic retinal axons distinguish between the two membrane preparations. Our results demonstrate that during axonal regeneration the mature neurons express embryonic properties that are involved in the recognition of tectal positional cues.

Keywords

RetinaRegenerationGanglion cellsPositional markersExplants
Type
Research Articles
Information
Visual Neuroscience , Volume 6 , Issue 6 , June 1991 , pp. 569 - 576
Copyright
Copyright © Cambridge University Press 1991

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