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A quantitative analysis of the effects of excitatory neurotoxins on retinal ganglion cells in the chick

Published online by Cambridge University Press:  02 June 2009

Ngoh Ngoh Tung ,
Ian G. Morgan  and
David Ehrlich
Ngoh Ngoh Tung
Affiliation:
Department of Anatomy, Monash University, Clayton, Vic 3168, Australia
Ian G. Morgan
Affiliation:
Visual Sciences Group, Research School of Biological Sciences, and Centre for Visual Sciences, Australian National University, Canberra, A.C.T. 26001, Australia
David Ehrlich
Affiliation:
Department of Anatomy, Monash University, Clayton, Vic 3168, Australia
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Abstract

The present study examines the differential effects of three excitotoxins, kainic acid (KA), N-methyl-D-aspartate (NMDA), and α-amino-2,3-amino-2,3-dihydro-5- methyl-3-oxo-4- isoxazolepropanoic acid (AMPA) on neurons within the genglion cell layer (GCL) of the chick retina. Two-day-old chicks were given a single, 5 μl, intravitreal injection of KA, NMDA, or AMPA at a range of doses. Following treatment with 40 nmol KA, there was a 21% loss of neurons in the GCL. At 200 nmol KA, the loss increased to 46%. Exposure to KA eliminated mainly small neurons of soma area 5–15μm2, and medium-sized ganglion cells of soma area 15–25μm2. Large ganglion cells (>25μ,2) remained unaffected. The vast majority of small cells were probably displaced amarcrine cells. At a does of 3000 nmol NMDA, no further loss of cells was evident. Exposure to 200 nmol AMPA resulted in a 30% loss of large and some medium-sized ganglion cells. In a further series of experiments, exposure to excitotoxin was followed by a retinal scratch, which eliminated retinal ganglion cells within the axotomized region. The results indicate that only a small proportion of displaced amacrine cells are destroyed by NMDA and AMPA, whereas virtually all displaced amarine cells are sensitive to KA. The findings of this study indicate the existence of subclasses of ganglion cells with specificity towards different types of excitatory amino acids (EAA).

Keywords

Retinal ganglion cellsKainic acidN-Methyl-D-aspartateα-amino-23-dihydro-5-methyl-3-oxo-4-isoxazolepropanoic acidExcitotoxicity
Type
Research Articles
Information
Visual Neuroscience , Volume 4 , Issue 3 , March 1990 , pp. 217 - 223
Copyright
Copyright © Cambridge University Press 1990

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References

Biziere, K. & Coyle, J.T. (1979). Localization receptors for kainic acid on neurons in the inner nuclear layer of retina. Neuropharmacology 18, 409–413.CrossRefGoogle Scholar
Boycott, B.B. & Wassle, H. (1974). The morphological types of ganglion cells of the domestic cat's retina. Journal of Physiology 240, 397–419.CrossRefGoogle ScholarPubMed
Cajal, S.R. (1892). La retine des vertebres. In La Cellule 9, 119–257.Google Scholar
English Translation entitled: The Structure of the Retina. Complied and translated by Sylvia, A. Thorpe & Glickstein, M., Springfield, Illinois: Thomas.Google Scholar
Ehrlich, D. (1981). Regional specialization of the chick retina as revealed by the size and density of neurons in the ganglion cell layer. Journal of Comparative Neurology 195, 643–657.CrossRefGoogle ScholarPubMed
Ehrlich, D., Keyser, K.T. & Karten, H.J. (1987 a). The origin and projection of substence P-containing ganglion cells in the chick retina. Journal of Comparative Neurology 266, 220–234.CrossRefGoogle Scholar
Ehrlich, D., Morgan, I.G. (1980). Kainic acid destroys displaced am acrine cells in posthatch chicken retina. Neuroscience Letters 17, 43–48.CrossRefGoogle Scholar
Ehrlich, D., Teuchert, G. & Morgan, I.G. (1987 b) Specific ganglion cell death induced by intravitreal kainic acid in the chicken retina. Brain Research 415, 342–346.CrossRefGoogle ScholarPubMed
Foster, A.C. & Fagg, G.E. (1984). Acidic amino-acid binding sites in mammalian neuronal membrances: their characteristics and religionship to synaptic receptors. Brain Research Reviews 7, 103–164.CrossRefGoogle Scholar
Fukuda, Y. (1977). A three-group classification of rat retinal ganglion cells: histological and physiological studies. Brain Research 119 327–344.CrossRefGoogle ScholarPubMed
Galvez, J.M.G., Peulles, L. & Prada, C. (1977). Inverted (displaced) retinal amacrine cells and their embryonic development in the chick. Experimental Neurology 56, 151–157.CrossRefGoogle ScholarPubMed
Gibson, B. & Reif-Lehrer, L. (1985). Mg2+ reduced N-methyl-D-as-partate neurotoxicity in embryonic chick neural retina in vitro. Neuroscience Letters 57, 13–17.CrossRefGoogle ScholarPubMed
Gomez-Ramos, P. & Perez-Rico, C. (1983). Morphological alteration of retinal cells accompanying inhibition of axonal transport by kainic acid. Exprimental Eye Research 36, 299–304.CrossRefGoogle ScholarPubMed
Hampton, C.K., Garcia, C. & Redburn, D.A. (1981). Localization of kainic acid-sensitive cells in the mammalian retina. Journal of Neuroscience Research 6, 99–111.CrossRefGoogle Scholar
Ingham, C.A. & Morgan, I.G. (1983). Does-dependent effects of intravitreal kainic acid on specific cell types in chicken retina. Neuroscience 9, 165–181.CrossRefGoogle Scholar
Kelly, J.P. & Gilbert, C.D. (1975). The projections of different morphological types of ganglion cells on the cat retina. Journal of Comparative Neurology 163, 65–80.CrossRefGoogle ScholarPubMed
Krogsgaard-Larsen, P., Honore, T. & Hansen, J.J. (1980). New class of glutamate agonist structurally related to ibotenic acid. Nature (London)284, 64–66.CrossRefGoogle ScholarPubMed
Kuljis, R.O. & Karten, H.J. (1982). Laminar organization of peptide like immunoreactivity in the anuran optic tectum. Journal of Comparative Neurology 212, 188–201.CrossRefGoogle ScholarPubMed
Levick, W.R. & Thibos, L.N. (1983). Spatial-frequency characteristics of brisk and sluggish ganglion cells of the cat's retina. Experimental Brain Research 51 (1), 16–22.Google Scholar
Millar, T.J., Ishimoto, I., Chubb, I.W., Epstein, M.L., Johnson, C.D. & Morgan, I.G. (1987). Cholinergic amacrine cells of the chicken retina: a light-and electron-microscope immunocytochemical study. Neuroscience 21, 725–743.CrossRefGoogle ScholarPubMed
Miller, R.F. & Slaughter, M.M. (1986). Excitatory amino-acid receptors of the retina: diversity of subtypes and conductance mechanisms. Trends in Neuroscinces 9, 211–218.CrossRefGoogle Scholar
Morgan, I.G. (1983). Kainic acid as a tool in retinal research. Progress in Retinal Research 2, 247–266.CrossRefGoogle Scholar
Morgan, I.G. (1984). Discrete cellular locations of excitatory amino-acid receptors in chicken retina. Neuroscience Letters (Suppl.) 15, 550.Google Scholar
Morgan, I.G. (1985). Quantitative properties of excitatory amino-acid receptor-mediated excitotoxicity in chicken retina. Neuroscience Letters (Suppl.) 19, 589.Google Scholar
Morgan, I.G. (1987). AMPA is a powerful neurotoxin in the chicken retina. Neuroscience Letters 79, 267–271.CrossRefGoogle ScholarPubMed
Nishimura, Y., Inoue, Y. & Shimai, K. (1979). Morphological development of retinal ganglion cells in the chick embryo. Exprimental Neurology 64, 44–60.CrossRefGoogle ScholarPubMed
Olney, J.W., Rhee, V. & Ho, O.L. (1974). Kainic acid: a powerful neurotoxic analogue to glutamate. Brain Research 77, 507–512.CrossRefGoogle ScholarPubMed
Rodieck, R.W. (1973). The Vertebrate Retina. San Francisco, California: Freeman Press.Google Scholar
Sattayasai, J., Rogers, L.J. & Ehrlich, D. (1985). Sequential treatment with low doses of kainic acid alters sensitivity of retinal cell types. Neuroscience Letters 54, 277–281.CrossRefGoogle ScholarPubMed
Sattayasai, J. & Ehrlich, D. (1987). Morphology of quisqualate-induced neurotoxicity in the chicken retina. Investigative Ophthalmology and Visual Science 28, 106–117.Google ScholarPubMed
Schwarcz, R. & Coyle, J.T. (1977). Kainic acid: neurotoxic effects after intraocular injection. Investigative Ophthalmology 16, 141–148.Google ScholarPubMed
Slaughter, M.M. & Miller, R.F. (1983). Bipolar cells in the mud-puppy retina use an excitatory amino acid neurotransmitter. Nature 303, 537–538.CrossRefGoogle Scholar
Stone, T.W. & Burton, N.R. (1988). NMDA receptors and ligands in the vertebrate CNS. Progress in Neurobiology 30, 333–368.CrossRefGoogle ScholarPubMed
Trejo, L.J. (1985). Retinal ganglion cell loss produced by intraocular kainic acid in rats: variation with some size and eccentricity. Brian Research 335, 221–230.CrossRefGoogle Scholar
Tung, N.N., Morgan, I.G. & Ehrlich, D. (1987). Intravitrea; kainic acid severely reduces the size of the developing optic rectum in newly hatched chickens. Brain Research 435, 153–159.CrossRefGoogle Scholar
Yazulla, S. & Kleinschmidt, J. (1980). The effects of intraocular injection of kainic acid on the synaptic organization of the goldfish retina. Brain Research 183, 287–301.CrossRefGoogle Scholar