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Normal photoresponses and altered b-wave responses to APB in the mdxCv3 mouse isolated retina ERG supports role for dystrophin in synaptic transmission

Published online by Cambridge University Press:  01 September 2004

DANIEL G. GREEN ,
HAO GUO  and
DE-ANN M. PILLERS
DANIEL G. GREEN
Affiliation:
Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, Michigan
HAO GUO
Affiliation:
Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, Michigan Current address: CTO, Howell Co. Ltd, 6 Yongding Xili Road, Beijing, China
DE-ANN M. PILLERS
Affiliation:
Departments of Pediatrics, Ophthalmology and Molecular and Medical Genetics, Doernbecher Children's Hospital, Casey Eye Institute, Oregon Health & Science University, Portland, Oegon
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Abstract

The mdxCv3 mouse is a model for Duchenne muscular dystrophy (DMD). DMD is an X-linked disorder with defective expression of the protein dystrophin, and which is associated with a reduced b-wave and has other electro- retinogram (ERG) abnormalities. To assess potential causes for the abnormalities, we recorded ERGs from pieces of isolated C57BL/6J and mdxCv3 mouse retinas, including measurements of transretinal and intraretinal potentials. The ERGs from the isolated mdxCv3 retina differ from those of control retinas in that they show reduced b-wave amplitudes and increased b-wave implicit times. Photovoltages obtained by recording across the photoreceptor outer segments of the retinas did not differ from normal, suggesting that the likely causes of the reduced b-wave are localized to the photoreceptor to ON-bipolar synapse. At a concentration of 50 μM, the glutamate analog DL-2-amino-4-phosphonobutyric acid (APB) blocks the b-wave component of the ERG, by binding to sites on the postsynaptic membrane. The On-bipolar cell contribution to the ERG was inferred by extracting the component that was blocked by APB. We found that this component was smaller in amplitude and had longer response latencies in the mdxCv3 mice, but was of similar overall time course. To assess the sensitivity of sites on the postsynaptic membrane to glutamate, the concentration of APB in the media was systematically varied, and the magnitude of blockage of the light response was quantified. We found that the mdxCv3 retina was 5-fold more sensitive to APB than control retinas. The ability of lower concentrations of APB to block the b-wave in mdxCv3 suggests that the ERG abnormalities may reflect alterations in either glutamate release, the glutamate postsynaptic binding sites, or in other proteins that modulate glutamate function in ON-bipolar cells.

Keywords

Isolated mdxCv3 mouse retinaIntraretinal ERGNormal light transductionAbnormal depolarizing bipolar cell activityHigh sensitivity to APB
Type
Research Article
Information
Visual Neuroscience , Volume 21 , Issue 5 , September 2004 , pp. 739 - 747
Copyright
2004 Cambridge University Press

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