Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T18:45:28.506Z Has data issue: false hasContentIssue false
  • English
  • Français

Green-sensitive cone photoreceptors are selectively labeled by Procion yellow dye in goldfish retina

Published online by Cambridge University Press:  02 June 2009

Heywood M. Petry  and
Carl J. Bassi
Heywood M. Petry
Affiliation:
Department of Psychology, State University of New York at Stony Brook, Stony Brook
Carl J. Bassi
Affiliation:
School of Optometry, University of Missouri at St. Louis, St. Louis
Get access Rights & Permissions [Opens in a new window]

Abstract

Selective labeling of intravitreal Procion yellow dye by presumed blue-sensitive cone photoreceptors has been demonstrated in primate retina. To determine whether Procion yellow is selective for this cone type in an unrelated vertebrate species, labeling by this dye was studied in goldfish retina, where cone pigment type can be directly inferred from photoreceptor morphology. At low vitreal concentrations of the dye (<0.4%), only cone outer segments were labeled. At vitreal concentrations of 0.4–0.5%, the inner segments of short-double cones and a subset of long single cones (presumed green-sensitive cones) were selectively stained. At still higher vitreal concentrations (0.6–0.7%), the inner segments of short-single cones and miniature short-single cones (presumed blue-sensitive cones) showed evidence of Procion label, but were not as heavily labeled. The inner segments of long-double cones and a subset of long-single cones (presumed red-sensitive cones) did not label at any of these concentrations. These results show that Procion yellow is not a selective marker for blue-sensitive cones in the goldfish retina. In addition, stained rod and cone nuclei were observed at each dye concentration, including those concentrations at which no inner segments were labeled.

Keywords

RetinaPhotoreceptorsConesGoldfishProcion yellow
Type
Research Article
Information
Visual Neuroscience , Volume 6 , Issue 1 , January 1991 , pp. 15 - 18
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahnelt, P.K., Kolb, H. & Pflug, R. (1987). Identification of a subtype of cone photoreceptor, likely to be blue sentive, in the human retina. Journal of Comparative Neurology 255, 18–34.CrossRefGoogle Scholar
Ahnelt, P.K. (1985). Characterization of the color-related receptor mosaic in the ground squirrel retina. Vision Research 25, 1557–1567.CrossRefGoogle ScholarPubMed
Bunt, A.H. & Klock, I.B. (1980 a). Fine structure and radioautography of retinal cone outer segments in goldfish and carp. Investigative Ophthalmology and Visual Science 19, 707–719.Google ScholarPubMed
Bunt, A.H. & Klock, I.B. (1980 b). Comparative study of [3H]-fu cose incorporation into vertebrate photoreceptor outer segments. Vision Research 20, 739–747.CrossRefGoogle Scholar
De, Monasterio F.M., Schein, S.J. & McCrane, E.P. (1981). Staining of blue-sensitive cones of the macaque retina by a fluorescent dye. Science 213, 1278–1281.Google Scholar
Johns, P.R. (1981). Growth of fish retinas. American Zoology 21, 447–458.CrossRefGoogle Scholar
Johnson, L.V., Hageman, G.S. & Blanks, J.C. (1986). Inter photoreceptor matrix domains ensheath vertebrate cone photoreceptor cells. Investigative Ophthalmology and Visual Science 27, 129–135.Google Scholar
Kouyama, N. & Marshak, D. (1990). Peptidergic bipolar cells selectively contact blue cones in the macaque monkey retina. Investigative Ophthalmology and Visual Science (Suppl) 31, 37.Google Scholar
Kuhnt, U., Kelly, M.J. & Schaumberg, R. (1979). Transynaptic transport of procion yellow in the central nervous system. Experimental Brain Research 35, 371–385.CrossRefGoogle ScholarPubMed
Laties, A.M., Bok, D. & Liebman, P. (1976). Procion yellow: a marker dye for outer segment disc patency and for rod renewal. Experimental Eye Research 23, 139–148.CrossRefGoogle ScholarPubMed
Laites, A.M. & Liebman, P. (1970). Cones of living amphibian eye: selective staining. Science 168, 1475–1477.CrossRefGoogle Scholar
Long, K.O. & Fisher, S.K. (1983). The distribution of photoreceptors and ganglion cells in the California ground squirrel (Spermophilus beecheyi). Journal of Comparative Neurology 221, 329–340.CrossRefGoogle ScholarPubMed
Marc, R.E. & Sperling, H.G. (1976 a). Color receptor identities of goldfish cones. Science 191, 487–489.CrossRefGoogle ScholarPubMed
Marc, R.E. & Sperling, H.G. (1976 b). The chromatic organization of the goldfish cone mosaic. Vision Research 16, 1211–1224.CrossRefGoogle ScholarPubMed
Marc, R.E. (1977). Chromatic patterns of cone photoreceptors. American Journal of Optometry and Physiological Optics 54, 212–225.CrossRefGoogle ScholarPubMed
Marc, R.E. (1982). Chromatic organization of the retina. In Cell Biology of the Eye, ed. McDevitt, D.S., pp. 435–473. New York: Academic Press.CrossRefGoogle Scholar
McCrane, E.P., de Monasterio, F.M., Schein, S.J. & Caruso, R.C. (1983). Non-fluorescent dye staining of primate blue cones. Investigative Ophthalmology and Visual Science 24, 1449–1455.Google ScholarPubMed
Petry, H.M. (1982). Selective staining of cells in tree shrew and gray squirrel retinae following intravitreal injection of Procion yellow dye. Neuroscience Abstracts 8, 131.Google Scholar
Petry, H.M. & Casagrande, V.A. (1983). Evidence for cone receptors in the galago retina. Investigative Ophthalmology and Visual Science (Suppl.) 24, 258.Google Scholar
Rohlich, P., Szel, A., Johnson, L.V. & Hageman, G.S. (1989). Carbohydrate components recognized by the cone-specific monoclonal antibody CSA-l and by peanut agglutinin are associated with red- and green-sensitive cone photoreceptors. Journal of Comparative Neurology 289, 395–400.CrossRefGoogle Scholar
Sperling, H.G., Harcombe, E.S. & Johnson, C. (1982). Stimulus-controlled labeling of cones in the macaque retina with [3H]-2-D-deoxy-glucose. In Structure of the Eye, Vol. IV, ed. Hollyfield, J.E., pp. 55–60. New York: Elsevier.Google Scholar
Sperling, H.G., Johnson, C. & Harwerth, R.S. (1980). Differential spectral photic damage to primate cones. Vision Research 20, 1117–1125.CrossRefGoogle ScholarPubMed
Stell, W.K. & Harosi, F.I. (1976). Cone structure and visual pigment content in the retina of the goldfish. Vision Research 16, 647–657.CrossRefGoogle ScholarPubMed
Szel, A., Dlamantstein, T. & Rohlich, P. (1988). Identification of the blue-sensitive cones in the mammalian retina by anti-visual pigment antibody. Journal of Comparative Neurology 273, 593–602.CrossRefGoogle ScholarPubMed
Wallis, G.L. (1942). The Vertebrate Eye and its Adaptive Radiations. New York: Hafner.Google Scholar
Wheeler, T.G. (1978). Goldfish retina: dorsal versus ventral areas. Vision Research 18, 1329–1336.CrossRefGoogle ScholarPubMed
Williams, D.R., Collier, R.J. & Thompson, B.J. (1983). Spatial resolution of the short-wavelength mechanism. In Colour Vision: Physiology and Psychophysics, ed. Mollon, J.D. & Sharpe, L.T., pp. 487–504. London: Academic Press.Google Scholar