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Expression of opsin mRNA in normal and vitamin A deficient retinas of the sphingid moth Manduca sexta

Published online by Cambridge University Press:  02 June 2009

Michael R. Chase ,
Ruth R. Bennett  and
Richard H. White
Michael R. Chase
Affiliation:
Department of Biology, University of Massachusetts at Boston, Boston
Ruth R. Bennett
Affiliation:
Department of Biology, University of Massachusetts at Boston, Boston
Richard H. White
Affiliation:
Department of Biology, University of Massachusetts at Boston, Boston
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Abstract

Two distinct opsin-encoding cDNAs, designated MANOP1 and MANOP2, were isolated as 3′ fragments from the sphingid moth Manduca sexta. They were obtained by reverse transcription of retinal RNA and amplification with the polymerase chain reaction (PCR) using a degenerate primer designed to an amino-acid sequence conserved in arthropod opsins. The cDNA fragments labelled bands at approximately 1.8 kb on Northern blots of retinal RNA extracts. Levels of opsin message were compared in retinas from normal moths, whose diets were fortified with carotenoid precursors of the Manduca rhodopsin chromophore, 3-hydroxyretinal, and those reared on carotenoid/retinoid (vitamin A) deficient diets. The chromophore-depleted retinas contained more opsin mRNA; this was particularly true for MANOP2. Thus, the chromophore is not required for opsin gene transcription in Manduca.

Keywords

Manduca sextaPhotoreceptorsOpsin cDNAOpsin mRNAVitamin A deficiency
Type
Research Articles
Information
Visual Neuroscience , Volume 13 , Issue 2 , March 1996 , pp. 353 - 358
Copyright
Copyright © Cambridge University Press 1996

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References

Balwdin, J.M. (1993). The probable arrangement of the helices in G protein-coupled receptors. EMBO Journal 12, 1693–1703.Google Scholar
Bennett, R.R. & Brown, P.K. (1985). Properties of the visual pigments of the moth Manduca sexta and the effects of two detergents, digitonin and CHAPS. Vision Research 25, 1771–1781.CrossRefGoogle Scholar
Bennett, R.R. & White, R.H. (1989). Influence of carotenoid deficiency on visual sensitivity, visual pigment and P-face particles of photoreceptor membrane in the moth Manduca sexta. Journal of Comparative Physiology A 164, 321–331.Google Scholar
Bennett, R.R. & White, R.H. (1991). 11-cis retinal restores visual function in vitamin A-deficient Manduca. Visual Neuroscience 6, 473–479.Google Scholar
Chang, B.S.W., Crandall, K.A., Carulli, J.P. & Hartl, D.L. (1995). Opsin phylogeny and evolution: A model for blue shifts in wavelength regulation. Molecular Phylogogeny and Evolution 4, 31–43.CrossRefGoogle Scholar
Cowman, A.F., Zuker, C.S. & Rubin, G.M. (1986). An opsin gene expressed in only one photoreceptor cell type of the Drosophila eye. Cell 44, 705–710.Google Scholar
Decouet, H.G. & Tanimura, T. (1987). Monoclonal antibodies provide evidence that rhodopsin in the outer rhabdomeres of Drosophila melanogaster is not glycosylated. European Journal of Cell Biology 44, 50–56.Google Scholar
Fryxell, K.J. & Meyerowitz, E.M. (1987). An opsin gene that is expressed only in the R7 photoreceptor cell of Drosophila. EMBO Journal 6, 443–451.Google Scholar
Hariyama, T., Ozaki, K., Tokunaga, F. & Tsukahara, Y. (1993). Primary structure of crayfish visual pigment deduced from cDNA. Federation of European Biochemical Societies 315, 287–292.Google Scholar
Huber, A., Wolfrum, U. & Paulsen, R. (1994). Opsin maturation and targeting to rhabdomeral photoreceptor membranes requires the retinal chromophore. European Journal of Cell Biology 44, 50–56.Google Scholar
Katz, M.L., Kutryb, M.J., Norberg, M., Gao, C.-L., White, R.H. & Stark, W.S. (1991). Maintenance of opsin density in photoreceptor outer segments of retinoid-deprived rats. Investigative Ophthalmology and Visual Science 32, 1968–1980.Google Scholar
Macdonald, R.J., Swift, G.H., Przybyla, A.E. & Chirgwin, J.M. (1987). Isolation of RNA using guanidinium salts. In Methods in Enzymology, Vol. 152, ed. Berger, S.L. & Kimmel, A.R., Chap. 20, pp. 219–227. San Diego: Academic Press.Google Scholar
Montell, C., Jones, K., Zuker, C. & Rubin, G. (1987). A second opsin gene expressed in the ultraviolet-sensitive R7 photoreceptor cells of Drosophila melanogaster. Journal of Neuroscience 7, 1558–1566.Google Scholar
Morriss-Kay, G., ed. (1992). Retinoids in Normal Development and Teratogenesis. New York: Oxford University Press.Google Scholar
O'tousa, J.E., Baehr, W., Martin, R.L., Hirsh, J., Pak, W.L. & Applebury, M.L. (1985). The Drosophila ninaE gene encodes an opsin. Cell 40, 839–850.Google Scholar
Ozaki, K., Nagatani, H., Ozaki, M. & Tokunaga, F. (1993). Maturation of major Drosophila rhodopsin, ninaE, requires chromophore 3-hydroxyretinal. Neuron 10, 1113–1119.Google Scholar
Paulsen, R. & Schwemer, J. (1983). Biogenesis of blowfly photoreceptor membranes is regulated by 11-cis retinal. European Journal of Biochemistry 137, 609–614.Google Scholar
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989). Molecular Cloning a Laboratory Manual, 2nd ed., Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Sapp, R.J., Christianson, J.S., Maier, L., Studer, K. & Stark, W.S. (1991). Carotenoid replacement therapy in Drosophila: Recovery of membrane, opsin and visual pigment. Experimental Eye Research 53, 73–79.Google Scholar
Schwemer, J. & Spengler, F. (1992). Opsin synthesis in blowfly photoreceptors is controlled by an 11-cis retinoid. In Structure and Functions of Retinal Proteins, ed. Rigaud, J.L., pp. 277–280. Colloques INSERM/John Libbey Eurotext, Montrouge.Google Scholar
Shukolyukov, S.A. & Denisova, N.A. (1992). Opsin biosynthesis and trans-cis isomerization of aldehyde form chromophore in the blow fly Calliphora erythrocephala eye. Insect Biochemistry and Molecular Biology 22, 925–935.Google Scholar
Smith, W.C., Price, D.A., Greenberg, R.M. & Battelle, B.-A. (1993). Opsins from the lateral eyes and ocelli of the horseshoe crab, Limulus polyphemus. Proceedings of the National Academy of Sciences of the U.S.A. 90, 6150–6154.CrossRefGoogle Scholar
Stark, W.S., Picking, W.L. & Vogt, M. (1995). Transcriptional control of Drosophila opsins by retinoids. Investigative Ophthalmology and Visual Science 36, S771.Google Scholar
Sun, D., Chen, D.-M., Harrelson, A. & Stark, W.A. (1993). Increased expression of chloramphenicol acetyltransferase by carotenoid and retinoid replacement in Drosophila opsin promoter fusion stocks. Experimental Eye Research 57, 177–187.Google Scholar
Tate, B.F., Levin, A.A. & Grippo, J.F. (1994). The discovery of 9-cis retinoic acid, a hormone that binds the retinoid-X receptor. Trends in Endocrinology and Metabolism 5, 189–194.Google Scholar
Towner, P. & GÄRtner, W. (1994). The primary structure of mantid opsin. Gene 143, 227–231.Google Scholar
White, R.H., Bard, J. & Bennett, R.R. (1991). Manduca opsin mRNA in relation to retinoids and light/dark cycle. Society for Neuroscience Abstracts 17, 640.Google Scholar
White, R.H. & Bennett, R.R. (1989). Ultrastructure of carotenoid deprivation in photoreceptors of Manduca sexta: Myeloid bodies and intracellular microvilli. Cell and Tissue Research 257, 519–528.CrossRefGoogle Scholar
White, R.H. & Bennett, R.R. (1992). Assembly of rhabdomeric membrane from smooth endoplasmic reticulum can be activated by light in chromophore-deprived photoreceptors of Manduca sexta. Cell and Tissue Research 270, 65–72.Google Scholar
White, R.H., Bennett, R.R & Chase, M. (1994). Opsin genes from Manduca and their expression in vitamin A deficiency. Society for Neuroscience Abstracts 20, 776.Google Scholar
White, R.H., Brown, P.K., Hurley, A.H. & Bennett, R.R. (1983). Rhodopsins, retinula cell ultrastructure and receptor potentials in the developing compound eye of the moth Manduca sexta. Journal of Comparative Physiology A 150, 153–163.Google Scholar
Zuker, C.S., Cowman, A.F. & Rubin, G.M. (1985). Isolation and structure of a rhodopsin gene from D. melanogaster. Cell 40, 851–858.Google Scholar
Zuker, C.S., Montell, C., Jones, K. & Rubin, G.M. (1987). Rhodopsin gene expressed in photoreceptor cell R7 of the Drosophila eye: Homologies with other signal-transducing molecules. Journal of Neuroscience 7, 1550–1557.Google Scholar