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Antibodies to human leucocyte antigens indicate subpopulations of microglia in human retina

Published online by Cambridge University Press:  02 June 2009

P.L. Penfold ,
M.C. Madigan  and
J.M. Provis
P.L. Penfold
Affiliation:
Save Sight and Eye Health Institute, Department of Ophthalmology, University of Sydney
M.C. Madigan
Affiliation:
Save Sight and Eye Health Institute, Department of Ophthalmology, University of Sydney
J.M. Provis
Affiliation:
Save Sight and Eye Health Institute, Department of Ophthalmology, University of Sydney Department of Anatomy, University of Sydney
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Abstract

Monoclonal antibodies to human leucocyte antigens, including anti-CD45 and anti-CD68, have been used to describe microglia in flatmounts of normal adult human retina for the first time. Anti-CD45 (the leucocyte common antigen) intensely labeled large numbers of cells in a regular distribution across the retina; anti-CD68 and anti-macrophage antibodies labeled fewer cells with distinctive morphologies, suggesting the presence of subpopulations of microglia in the human retina expressing leucocyte antigens.

Keywords

Human retinaMicrogliaCD antigensSubpopulationsRetinal flatmounts
Type
Short Communications
Information
Visual Neuroscience , Volume 7 , Issue 4 , October 1991 , pp. 383 - 388
Copyright
Copyright © Cambridge University Press 1991

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References

Adams, J.C. (1981). Heavy metal intensification of DAB-based HRP reaction product. Journal of Histochemistry and Cytochemistry 29, 775–778.CrossRefGoogle ScholarPubMed
Akiyama, H., Itagaki, S. & McGeer, P.L. (1988). Major histocompatibility complex antigen expression on rat microglia following epidural kainic-acid lesions. Journal of Neuroscience Research 20, 147–157.CrossRefGoogle ScholarPubMed
Ashwell, K. (1989). Development of microglia in the albino rabbit retina. Journal of Comparative Neurology 287, 286–301.CrossRefGoogle ScholarPubMed
Ashwell, K.W.S., Hollander, H., Streit, W. & Stone, J. (1989). The appearance and distribution of microglia in the developing retina of the rat. Visual Neuroscience 2, 437–448.CrossRefGoogle ScholarPubMed
Beverley, P.C.L., Merkenschlager, M. & Terry, L. (1988). Phenotypic diversity of the CD45 antigen and its relationship to function. Immunology (Suppl.), 1, 3–5.Google ScholarPubMed
Boya, J., Calvo, J. & Carbonell, A.L. (1987). Appearance of microglial cells in the postnatal rat retina. Archives of Histology Japonica 50, 223–228.CrossRefGoogle ScholarPubMed
Boycott, B.B. & Hopkins, J.M. (1981). Microglia in the retina of monkey and other mammals: its distinction from other types of glia and horizontal cells. Neuroscience 6, 679–688.CrossRefGoogle ScholarPubMed
Rio-Hortega, P.Del (1919). Poder fagocitario y mobilidad del la microglia. Boletin de la Sociedad Espanola de Biologia 9, 154–171.Google Scholar
Dickson, D. & Mattiace, L.A. (1989). Astrocytes and microglia in human brain share an epitope recognized by a B-lymphocyte-specific monoclonal antibody (LN-1). American Journal of Pathology 135, 135–147.Google ScholarPubMed
Erber, W. (1990). Human leucocyte differentiation antigens: review of the CD nomenclature. Pathology 22, 61–69.CrossRefGoogle ScholarPubMed
Esiri, M.M. & McGee, J. O'D. (1986). Monoclonal antibody to macrophages (EMB/11) labels macrophages and microglial cells in human brain. Journal of Clinical Pathology 39, 615–621.CrossRefGoogle ScholarPubMed
Ferrer, I. & Sarmiento, J. (1980). Reactive microglia in the developing brain. Acta Neuropathologica 50, 69–76.CrossRefGoogle ScholarPubMed
Franklin, W.A., Mason, D.Y., Pulford, K., Falini, B., Bliss, E., Gatter, K.C., Stein, H., Clarke, L.C. & McGee, J. O'D. (1986). Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Laboratory Investigation 54, 332–335.Google ScholarPubMed
Fujita, S. & Kitamura, T. (1976). Origin of brain macrophages and the nature of microglia. In Progress in Neuropathology, ed. Zimmerman, H.M., pp. 1–50. New York: Grune & Stratton.Google Scholar
Giulian, D. & Baker, T.J. (1986). Characterization of amoeboid microglia isolated from developing mammalian brain. Journal of Neuroscience 6, 2163–2178.CrossRefGoogle Scholar
Hayes, G.M., Woodroofe, M.N. & Cuzner, M.L. (1988). Characterization of microglia isolated from adult human and rat brain. Journal of Neuroimmunology 19, 177–189.CrossRefGoogle ScholarPubMed
Hickey, W.F. & Kimura, H. (1988). Perivascular microglial cells on the CNS are bone marrow-derived and present antigen in vivo. Science 239, 290–292.CrossRefGoogle ScholarPubMed
Honda, H., Kimura, H., Silvers, W.K. & Rostami, A. (1990). Perivascular location and phenotypic heterogeneity of microglial cells in the rat brain. Journal of Neuroimmunology 29, 183–191.CrossRefGoogle ScholarPubMed
Hume, D.A., Perry, V.H. & Gordon, S. (1983). Immunohistochemical localization of macrophage specific antigen in developing mouse retina: phagocytosis of dying neurons and differentiation of microglia to form a regular array in the plexiform layers. Journal of Cellular Biology 97, 253–257.CrossRefGoogle Scholar
Jordan, F.L. & Thomas, W.E. (1988). Brain macrophages: questions of origin and interrelationship. Brain Research Reviews 13, 165–178.CrossRefGoogle Scholar
Kitamura, T., Tsuchihashi, Y., Tatebe, A. & Fujita, S. (1977). Electron microscopic features of the resting microglia in the rabbit hippocampus identified by silver carbonate stain. Acta Neuropathologica 38, 195–201.CrossRefGoogle Scholar
Kitamura, T., Miyake, T. & Fujita, S. (1984). Genesis of resting microglia in the gray matter of mouse hippocampus. Journal of Comparative Neurology 226, 421–433.CrossRefGoogle ScholarPubMed
Linden, R., Cavalcante, L.A. & Barradas, P.C. (1986). Mononuclear phagocytes in the retina of developing rats. Histochemistry 85, 335–339.CrossRefGoogle ScholarPubMed
Ling, E.A. (1981). The origin and nature of microglia. Advances in Cellular Neurobiology 2, 33–82.CrossRefGoogle Scholar
Lowe, J., Maclennan, K.A., Powe, D.G., Pound, J.D. & Palmer, J.B. (1989). Microglial cells in human brain have phenotypic characteristics related to possible function as dendritic antigen presenting cells. Journal of Pathology 159, 143–149.CrossRefGoogle ScholarPubMed
Mannoji, H., Yeger, H. & Becker, L.E. (1986). A specific histochemical marker (lectin ricinus communis agglutinin-1) for normal human microglia. Journal of Neuropathology and Experimental Neurology 45, 345.CrossRefGoogle Scholar
Miles, J.M. & Chou, S.M. (1988). A new immunoperoxidase marker for microglia in paraffin section. Journal of Neuropathology and Experimental Neurology 47, 579–587.CrossRefGoogle ScholarPubMed
Murabe, Y. & Sano, Y. (1981). Thiamine pyrophosphatase activity in the plasma membrane of microglia. Histochemistry 71, 45–52.CrossRefGoogle ScholarPubMed
Murabe, Y. & Sano, Y. (1983). Morphological studies on neuroglia VII. Distribution of “brain macrophages” in brains of neonatal and adult rats, as determined by means of immunohistochemistry. Cell and Tissue Research 229, 85–95.Google ScholarPubMed
Oehmichen, M., Wietholter, H. & Greaves, M.F. (1979). Immunological analysis of human microglia: lack of monocytic and lymphoid membrane differentiation antigens. Journal of Neuropathology and Experimental Neurology 38, 99–103.CrossRefGoogle ScholarPubMed
Oehmichen, M. (1982). Are resting and/or reactive microglia macrophages?. Immunobiology 161, 246–254.CrossRefGoogle ScholarPubMed
Penfold, P.L., Provis, J.M., Madigan, M.C., Driel, D.Van & Billson, F.A. (1990). Angiogenesis in normal human retinal development: the involvement of astrocytes and macrophages. Graefe's Archive for Clinical and Experimental Ophthalmology 228, 255–263.CrossRefGoogle ScholarPubMed
Perry, V.H. & Gordon, S. (1988). Macrophages and microglia in the nervous system. Trends in Neuroscience 11, 273–277.CrossRefGoogle ScholarPubMed
Perry, V.H. & Lund, R.D. (1989). Microglia in retinae transplanted to the central nervous system. Neuroscience 31, 453–462.CrossRefGoogle ScholarPubMed
Poltorak, M. & Freed, W.J. (1989). Immunological reactions induced by intracerebral transplantation: evidence that host microglia but not astroglia are the antigen-presenting cells. Experimental Neurology 103, 222–233.CrossRefGoogle Scholar
Prickett, T.C.R. & Hart, D.N.J. (1990). Anti-leucocyte common (CD45) antibodies inhibit dendritic cell stimulation of CD4 and CD8 T-lymphocyte proliferation. Immunology 69, 250–256.Google ScholarPubMed
Schelper, R.L. & Adrian, E.K. Jr., (1986). Monocytes become macrophages: they do not become microglia: A light and electron microscopic autoradiographic study using 125-iododeoxyuridine. Journal of Neuropathology and Experimental Neurology 45, 1–19.CrossRefGoogle Scholar
Schnitzer, J. (1989). Enzyme-histochemical demonstration of microglial cells in the adult and postnatal rabbit retina. Journal of Comparative Neurology 282, 249–263.CrossRefGoogle ScholarPubMed
Sminia, T., Groot, C.J.A.De, Dijkstra, C.D., Koetsier, J.C. & Polman, C.H. (1987). Macrophages in the central nervous system of the rat. Immunobiology 174, 43–50.CrossRefGoogle ScholarPubMed
Stone, J. & Dreher, Z. (1987). Relationship between astrocytes, ganglion cells and vasculature of the retina. Journal of Comparative Neurology 255, 35–49.CrossRefGoogle ScholarPubMed
Streit, W.J. & Kreutzberg, G.W. (1987). Lectin binding by resting and reactive microglia. Journal of Neurocytology 16, 249–260.CrossRefGoogle ScholarPubMed
Streit, W.J., Graeber, M.B. & Kreutzberg, G.W. (1988). Functional plasticity of microglia: a review. Glia I, 301–307.CrossRefGoogle Scholar
Suzuki, H., Franz, H., Yamamoto, T., Iwasaki, Y. & Konno, H. (1988). Identification of the normal microglial population in human and rodent nervous tissue using lectin-histochemistry. Neuropathology and Applied Neurobiology 14, 221–227.CrossRefGoogle ScholarPubMed
Vrabec, F. (1970). Microglia in the monkey and rabbit retina. Journal of Neuropathology and Neurology 29, 217–224.Google ScholarPubMed
Wood, G.W., Gollahon, K.A., Tilzer, S.A., Vats, T. & Morantz, R.A. (1979). The failure of microglia in normal brain to exhibit mononuclear phagocyte markers. Journal of Neuropathology and Experimental Neurology 38, 369–376.CrossRefGoogle ScholarPubMed
Zucker-Franklin, D., Warfel, A., Grusky, G., Frangione, B. & Teitel, D. (1987). Novel monocyte-like properties of microglial/astroglial cells, constitutive secretion of lysozyme and cytastatin-C. Laboratory Investigation 57, 176–185.Google Scholar