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Anesthesia can cause sustained hyperglycemia in C57/BL6J mice

Published online by Cambridge University Press:  06 December 2005

E.T. BROWN ,
Y. UMINO ,
T. LOI ,
E. SOLESSIO  and
R. BARLOW
E.T. BROWN
Affiliation:
Center for Vision Research, SUNY Upstate Medical University, Syracuse
Y. UMINO
Affiliation:
Center for Vision Research, SUNY Upstate Medical University, Syracuse
T. LOI
Affiliation:
Center for Vision Research, SUNY Upstate Medical University, Syracuse
E. SOLESSIO
Affiliation:
Center for Vision Research, SUNY Upstate Medical University, Syracuse
R. BARLOW
Affiliation:
Center for Vision Research, SUNY Upstate Medical University, Syracuse
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Abstract

Effects of anesthesia on the blood glucose of C57/BL6J mice were evaluated under conditions commonly used for testing retinal sensitivity with electroretinographic (ERG) recordings. We evaluated the effects of four anesthetics: nembutal (50 mg/kg), pentothal (100 mg/kg), avertin (240 mg/kg), and ketamine/xylazine (100 mg/kg) using saline as control. We measured blood glucose (BG) levels from tail vein blood before and 15 and 60 min following intraperitoneal injections. Fifteen minutes postinjection, all four anesthetics and saline elevated BG with ketamine/xylazine and avertin having substantially greater effects than nembutal, pentothal, and saline. Only the effects of ketamine/xylazine and avertin persisted throughout the test period. Sixty minutes after injecting ketamine/xylazine BG remained elevated at 400 ± 42 mg/dl, a 167% increase over preinjection levels. Sixty minutes after injecting avertin BG was 288 ± 10 mg/dl, a 59% increase over preinjection levels. No sustained elevation in BG was detected 60 min following injection of nembutal, pentothal, or saline. Because BG can affect the amplitude of the ERG, caution should be exercised in the use of ketamine/xylazine or avertin. The choice of anesthesia may also be important in diabetes and metabolism research where changes in blood glucose could impact physiological processes.

Keywords

AnesthesiaBlood glucose levelsERGMice
Type
Research Article
Information
Visual Neuroscience , Volume 22 , Issue 5 , September 2005 , pp. 615 - 618
Copyright
© 2005 Cambridge University Press

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