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Activation of glutamate transporters in rods inhibits presynaptic calcium currents

Published online by Cambridge University Press:  23 January 2004

KATALIN RABL ,
ERIC J. BRYSON  and
WALLACE B. THORESON
KATALIN RABL
Affiliation:
Department of Ophthalmology, University of Nebraska Medical Center, Omaha On leave from the Department of General Zoology and Neurobiology, University of Pécs, Pécs, Hungary
ERIC J. BRYSON
Affiliation:
Department of Ophthalmology, University of Nebraska Medical Center, Omaha
WALLACE B. THORESON
Affiliation:
Department of Ophthalmology, University of Nebraska Medical Center, Omaha Department of Pharmacology, University of Nebraska Medical Center, Omaha
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Abstract

We found that L-glutamate (L-Glu) inhibits L-type Ca2+ currents (ICa) in rod photoreceptors. This inhibition was studied in isolated rods or rods in retinal slices from tiger salamander using perforated patch whole cell recordings and Cl−-imaging techniques. Application of L-Glu inhibited ICa by ∼20% at 0.1 mM and ∼35% at 1 mM. L-Glu also produced an inward current that reversed around ECl. The metabotropic glutamate receptor (mGluR) agonists t-ADA (Group I), DCG-IV (Group II), and L-AP4 (Group III) had no effect on ICa. However, the glutamate transport inhibitor, TBOA (0.1 mM), prevented L-Glu from inhibiting ICa. D-aspartate (D-Asp), a glutamate transporter substrate, also inhibited ICa with significantly more inhibition at 1 mM than 0.1 mM. Using Cl− imaging, L-Glu (0.1–1 mM) and D-Asp (0.1–1 mM) were found to stimulate a Cl− efflux from terminals of isolated rods whereas the ionotropic glutamate receptor agonists NMDA, AMPA, and kainate and the mGluR agonist, 1S,3R-ACPD, did not. Glutamate-evoked Cl− effluxes were blocked by the glutamate transport inhibitors TBOA and DHKA. Cl− efflux inhibits Ca2+ channel activity in rod terminals (Thoreson et al. (2000), Visual Neuroscience17, 197). Consistent with the possibility that glutamate-evoked Cl− efflux may play a role in the inhibition, reducing intraterminal Cl− prevented L-Glu from inhibiting ICa. In summary, the results indicate that activation of glutamate transporters inhibits ICa in rods possibly as a consequence of Cl− efflux. The neurotransmitter L-Glu released from rod terminals might thus provide a negative feedback signal to inhibit further L-Glu release.

Keywords

PhotoreceptorL-type calcium channelRetinaGlutamate transporterTiger salamander
Type
Research Article
Information
Visual Neuroscience , Volume 20 , Issue 5 , September 2003 , pp. 557 - 566
Copyright
2003 Cambridge University Press

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