Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-20T09:17:35.091Z Has data issue: false hasContentIssue false
  • English
  • Français

Stringent Programming of DNA Methylation in Humans

Published online by Cambridge University Press:  21 February 2012

Hnin T. Aung ,
Dion K. Harrison ,
Ian Findlay ,
John S. Mattick ,
Nicholas G. Martin  and
Bernard J. Carroll
Hnin T. Aung
Affiliation:
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia.
Dion K. Harrison
Affiliation:
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia.
Ian Findlay
Affiliation:
Australian Genome Research Facility, The University of Queensland, Australia; Institute for Molecular Bioscience, The University of Queensland, Australia.
John S. Mattick
Affiliation:
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia; Institute for Molecular Bioscience, The University of Queensland, Australia.
Nicholas G. Martin
Affiliation:
Queensland Institute of Medical Research, Brisbane, Australia.
Bernard J. Carroll*
Affiliation:
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia; Institute for Molecular Bioscience, The University of Queensland, Australia. [email protected]
*
*Address for correspondence: Bernard J. Carroll, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia.

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

We describe a PCR-based method called Amplified Methylation Polymorphism (AMP) for scanning genomes for DNA methylation changes. AMP detects tissue-specific DNA methylation signatures often representing junctions between methylated and unmethylated DNA close to intronexon junctions and/or associated with CpG islands. Identical AMP profiles are detected for healthy, young, monozygotic twins.

Keywords

DNA methylationmethylation-sensitive PCRtissue-specificidentical twins
Type
Articles
Information
Twin Research and Human Genetics , Volume 13 , Issue 5 , 01 October 2010 , pp. 405 - 411
Copyright
Copyright © Cambridge University Press 2010