Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-19T06:45:14.227Z Has data issue: false hasContentIssue false
  • English
  • Français

Genotyping Accuracy for Whole-Genome Amplification of DNA from Buccal Epithelial Cells

Published online by Cambridge University Press:  21 February 2012

Keren Leviel ,
Megan Olarte  and
Patrick F. Sullivan
Keren Leviel
Affiliation:
Department of Genetics, University of North Carolina at Chapel Hill, United States of America.
Megan Olarte
Affiliation:
Department of Genetics, University of North Carolina at Chapel Hill, United States of America.
Patrick F. Sullivan*
Affiliation:
Department of Genetics, University of North Carolina at Chapel Hill, United States of America. [email protected]
*
*Address for correspondence: Dr Sullivan, Department of Genetics, CB#7264, 4109 Neurosciences Research Building, University of North Carolina, Chapel Hill, NC, 27599-7264, USA.

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

We compared the accuracy of genotyping for DNA extracted from lymphocytes to that of DNA amplified from buccal epithelial cells. Amplification was via a rolling circle/φ29 DNA polymerase commercial kit. Paired buccal and lymphocyte DNA samples were available from 30 individuals. All samples were genotyped for 12 SNPs, 5 microsatellites and 2 VNTRs. The accuracy of genotyping (no-call proportions, reproducibility, and concordance) was similar for DNA from lymphocytes in comparison to amplified DNA from buccal samples. If used with caution, these data suggest that rolling-circle whole-genome amplification can be used to increase the DNA mass available for large-scale genotyping projects based on DNA from buccal cells.

Type
Articles
Information
Twin Research and Human Genetics , Volume 7 , Issue 5 , 01 October 2004 , pp. 482 - 484
Copyright
Copyright © Cambridge University Press 2004