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Glass-based nanofluidic device for biomolecule preconcentration study

Published online by Cambridge University Press:  24 October 2008

P. Svarnas ,
A. Plecis ,
C. Nanteuil ,
D. Duong ,
C. David ,
M. Muller  and
Y. Chen
P. Svarnas*
Affiliation:
Laboratoire de Photonique et de Nanostructures, LPN-CNRS, Route de Nozay, 91460 Marcoussis, France
A. Plecis
Affiliation:
Laboratoire de Photonique et de Nanostructures, LPN-CNRS, Route de Nozay, 91460 Marcoussis, France
C. Nanteuil
Affiliation:
Laboratoire de Photonique et de Nanostructures, LPN-CNRS, Route de Nozay, 91460 Marcoussis, France
D. Duong
Affiliation:
Laboratoire de Photonique et de Nanostructures, LPN-CNRS, Route de Nozay, 91460 Marcoussis, France
C. David
Affiliation:
Laboratoire de Photonique et de Nanostructures, LPN-CNRS, Route de Nozay, 91460 Marcoussis, France
M. Muller
Affiliation:
Laboratoire pour l'Application de Lasers de Puissance, LALP-CNRS/GIP-GERAILP, 16 bis av. Prieur de la Côte d'Or, 94114 Arcueil Cedex, France
Y. Chen
Affiliation:
Laboratoire de Photonique et de Nanostructures, LPN-CNRS, Route de Nozay, 91460 Marcoussis, France
*
[email protected]
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Abstract

The fabrication of a hybrid micro/nano-fluidic device to study electropreconcentration of biomolecules is presented. A nanoslit surrounded by glass is formed between two access microchannels and an “H” configuration bio-analysis system is developed. The optimized know-how for this biochip fabrication is transferred and the key steps are discussed. Fluorescence spatiotemporal profile in the preconcentration area is recorded and quantitatively analyzed. The reliability of the applied technology for studies of charged biomolecule transport phenomena across nanochannels is proven giving two examples for different preconcentration geometries.

Keywords

Type
Research Article
Information
The European Physical Journal - Applied Physics , Volume 44 , Issue 3 , December 2008 , pp. 245 - 253
Copyright
© EDP Sciences, 2008

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