Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-27T06:16:30.676Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of mechanical properties of fixed bone cells with sub-micrometer thickness by picosecond ultrasonics

Published online by Cambridge University Press:  14 January 2013

Mathieu Ducousso ,
Omar El-Farouk Zouani ,
Christel Chanseau ,
Céline Chollet ,
Clément Rossignol ,
Bertrand Audoin  and
Marie-Christine Durrieu
Mathieu Ducousso
Affiliation:
University Bordeaux, I2M, UMR 5295, 33400 Talence, France CNRS, I2M, UMR 5295, 33400 Talence, France
Omar El-Farouk Zouani
Affiliation:
University Bordeaux, INSERM U1026, 33076 Bordeaux, France
Christel Chanseau
Affiliation:
University Bordeaux, INSERM U1026, 33076 Bordeaux, France
Céline Chollet
Affiliation:
University Bordeaux, INSERM U1026, 33076 Bordeaux, France
Clément Rossignol*
Affiliation:
University Bordeaux, I2M, UMR 5295, 33400 Talence, France CNRS, I2M, UMR 5295, 33400 Talence, France
Bertrand Audoin
Affiliation:
University Bordeaux, I2M, UMR 5295, 33400 Talence, France CNRS, I2M, UMR 5295, 33400 Talence, France
Marie-Christine Durrieu
Affiliation:
University Bordeaux, INSERM U1026, 33076 Bordeaux, France
*
ae-mail: [email protected]
Get access

Abstract

The picosecond ultrasonics technique is used to investigate the viscoelastic properties of nucleus of fixed single osteoblast progenitor cells adhering on a titanium alloy substrate. A two-color probing picosecond ultrasonics and a fluorescence visualization setups were developed and combined to allow to distinguish subcomponents inside the cell under investigation. It opens the way for quantitative measurements of the viscoelastic properties of single cells and of their sub-micrometer thickness. It is shown that a blue probe, λ = 400 nm, is preferable to a red probe, λ = 800 nm, to perform these measurements with fixed sub-micrometer bone cells. 26 GHz acoustic frequencies are detected in cells as thin as 135 nm. A 1D analytical model of the acoustic generation and of the optical detection is used to describe the experimental results. The nucleus longitudinal elastic moduli (13–16 GPa) and dynamic viscosities (13–30 cP) are measured at high frequencies (GHz) from a time-frequency analysis of the experimental data of fixed single cells.

Type
Research Article
Information
The European Physical Journal - Applied Physics , Volume 61 , Issue 1 , January 2013 , 11201
Copyright
© EDP Sciences, 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cross, S.E., Jin, Y.-S., Rao, J., Gimzewski, J.K., Nature Nanotechnol. 2, 780 (2007)CrossRef
Anselme, K., Biomater. 21, 667 (2000)CrossRef
Rotsch, C., Radmacher, M., Biophys. J. 78, 520 (2000)CrossRef
Van Vliet, K.J., Bao, G., Suresh, S., Acta Mater. 51, 5881 (2003)CrossRef
Lim, C.T., Zhou, E.H., Li, A., Vedula, S.R.K., Fu, H.X., Mat. Sci. Eng.: C 26, 1278 (2006)CrossRef
Gennisson, J.L., Baldeweck, T., Tanter, M., Catheline, S., Fink, M., Sandrin, L., Cornillon, C., Querleux, B., IEEE Trans. Ultrason. Ferroelectr. Freq. Control 51, 980 (2004)CrossRef
Wells, P.N.T., Liang, H.D., J. R. Soc. Interface 8, 1521 (2011)CrossRef
Bereiter-Hahn, J., Blase, C., Ultrasonic Characterization of Biological Cells (CRC Press, Boca Raton, FL, 2003)CrossRefGoogle Scholar
Lemor, R.M., Weiss, E., Zinin, P., Pilarczyk, G., IEEE Int. Ultrason. Symp. Proc. 1, 622 (2004)
Karl, I., Bereiter-Hahn, J., Cell Biochem. Biophys. 29, 225 (1998)CrossRef
Thomsen, C., Strait, J., Vardeny, Z., Maris, H.J., Tauc, J., Hauser, J.J., Phys. Rev. Lett. 53, 989 (1984)CrossRef
Antonelli, G.A., Perrin, B., Daly, B.C., Cahill, D.G., MRS Bull. 31, 607 (2006)CrossRef
Scherbakov, A.V., Salasyuk, A.S., Akimov, A.V., Liu, X., Bombeck, M., Brüggemann, C., Yakovlev, D.R., Sapega, V.F., Furdyna, J.K., Bayer, M., Phys. Rev. Lett. 105, 117204 (2010)CrossRef
Thevenard, L., Peronne, E., Gourdon, C., Testelin, C., Cubukcu, M., Charron, E., Vincent, S., Lemaître, A., Perrin, B., Phys. Rev. B 82, 104422 (2010)CrossRef
Yang, F., Atay, T., Dang, C.H., Grimsley, T.J., Che, S., Ma, J., Zhang, Q., Nurmikko, A.V., Maris, H.J., J. Phys.: Conf. Ser. 92, 012024 (2007)
Pezeril, T., Klieber, C., Andrieu, S., Nelson, K.A., Phys. Rev. Lett. 102, 107402 (2009)CrossRef
Decremps, F., Belliard, L., Perrin, B., Gauthier, M., Phys. Rev. Lett. 100, 035502 (2008)CrossRef
Rossignol, C., Chigarev, N., Ducousso, M., Audoin, B., Forget, G., Guillemot, F., Durrieu, M.C., Appl. Phys. Lett. 93, 123901 (2008)CrossRef
Audoin, B., Rossignol, C., Chigarev, N., Ducousso, M., Forget, G., Guillemot, F., Durrieu, M.C., Ultrasonics 50, 202 (2010)CrossRef
Ducousso, M., Dehoux, T., Rossignol, C., Audoin, B., Zouani, O., Chollet, C., Chanseau, C., Durrieu, M.C., J. Phys.: Conf. Ser. 278, 012042 (2011)
Audoin, B., Ducousso, M., Dehoux, T., Chollet, C., Zouani, O., Chanseau, C., Durrieu, M.-C., Photons Plus Ultrasound: Imaging and Sensing 78990X (2011)
Perrin, B., Microscale and nanoscale heat transfer (Springer, Berlin, 2007)Google Scholar
Verdier, C., Etienne, J., Duperray, A., Preziosi, L., C. R. Phys. 10, 790 (2009)CrossRef
Starodubtseva, M.N., Ageing Res. Rev. 10, 16 (2011)CrossRef
Rouch, J., Lai, C.C., Chen, S.H., J. Chem. Phys. 65, 4016 (1976)CrossRef
Yang, F., Grimsley, T.J., Che, S., Antonelli, G.A., Maris, H.J., Nurmikko, A.V., J. Appl. Phys. 107, 103537 (2010)CrossRef
Auld, B.A., Acoustic Fields and Waves in Solids, vol. 1 (Robert E. Krieger Publishing Company, 1990)Google Scholar
Thomsen, C., Grahn, H.T., Maris, H.J., Tauc, J., Phys. Rev. B 34, 4129 (1986)CrossRef
Devos, A., Robillard, J.F., Côte, R., Emery, P., Phys. Rev. B 74, 064114 (2006)CrossRef
Kashiwada, S., Matsuda, O., Baumberg, J.J., Li Voti, R., Wright, O.B., J. Appl. Phys. 100, 073506 (2006)CrossRef
Ducousso, M., Dehoux, T., Audoin, B., Zouani, O., Chollet, C., Durrieu, M.C., J. Phys.: Conf. Ser. 269, 012024 (2011)
Auld, B.A., Acoustic Fields and Waves in Solids (Wiley, New York 1973)Google Scholar
Ducousso, M., Dehoux, T., Audoin, B., Zouani, O., Chollet, C., Durrieu, M.-C., J. Phys.: Conf. Ser. 269, 012024 (2010)
Rossignol, C., Perrin, B., Laborde, S., Vandenbulcke, L., Barros, M.I.D., Djemia, P., J. Appl. Phys. 95, 4157 (2004)CrossRef
Wright, O.B., J. Appl. Phys. 71, 1617 (1992)CrossRef
Dehoux, T., Chigarev, N., Rossignol, C.M., Audoin, B., Phys. Rev. B 76, 024311 (2007)CrossRef
Wright, O., Perrin, B., Matsuda, O., Gusev, V.E., Phys. Rev. B 78, 024303 (2008)CrossRef
Dil, J.G., Rep. Prog. Phys. 45, 285 (1982)CrossRef
Born, M., Wolf, E., Principles of Optics (Cambridge University Press, 1998)Google Scholar
Houser, G.D., Garmire, E., Appl. Opt. 33, 1059 (1994)CrossRef
Werley, C.A., Teo, S.M., Nelson, K.A., Rev. Sci. Instrum. 82, 123108 (2011)CrossRef
Long, M., Rack, H.J., Biomater. 19, 1621 (1998)CrossRef
Zouani, O.F., Chollet, C., Guillotin, B., Durrieu, M.-C., Biomater. 31, 8245 (2010)CrossRef
Beuthan, J., Minet, O., Helfmann, J., Herrig, M., Müller, G., Phys. Med. Biol. 41, 369 (1996)CrossRef
Hatton, J.P., Pooran, M., Li, C.F., Luzzio, C., Hughes-Fulford, M., J. Bone Miner. Res. 18, 58 (2003)CrossRef
Caille, N., Thoumine, O., Tardy, Y., Meister, J.-J., J. Biomech. 35, 177 (2002)CrossRef
Pontecorvo, E., Ortolani, M., Polli, D., Ferretti, M., Ruocco, G., Cerullo, G., Scopigno, T., Appl. Phys. Lett. 98, 011901 (2011)CrossRef