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Characterization of microporous membranes using confocal scanning laser microscopy influorescence mode

Published online by Cambridge University Press:  15 December 2000

C. Charcosset  and
J.-C. Bernengo
C. Charcosset*
Affiliation:
Laboratoire d'Automatique et de Génie des Procédés, UCB Lyon 1, UPRES A-Q 5007 CNRS, ESCPE-Lyon, Bât. 308 G, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
J.-C. Bernengo
Affiliation:
UCB Lyon 1, Centre Commun de Quantimétrie, 8 avenue Rockfeller, 69373 Lyon Cedex 08, France
*
[email protected]
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Abstract

Confocal Scanning Laser Microscopy (CSLM) in fluorescence mode was used to characterize microporous membranes. Two microfiltration membranes were investigated: a mixed ester (cellulose nitrate/cellulose acetate) 1.2 μm-rated membrane and a polycarbonate track-etched membrane with cylindrical pores of 2 μm diameter. Optical sections of the membranes stained with rhodamine and mounted in glycerol were performed at 1 μm intervals, from 0 to 10 μm. CSLM was found useful for microporous membrane characterization, as it gives some insight into bulk membrane morphology.

Keywords

Type
Research Article
Information
The European Physical Journal - Applied Physics , Volume 12 , Issue 3 , December 2000 , pp. 195 - 199
Copyright
© EDP Sciences, 2000

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