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Some biomedical applications of ferrofluids*

Published online by Cambridge University Press:  15 March 1999

J. Roger ,
J. N. Pons ,
R. Massart ,
A. Halbreich  and
J. C. Bacri
J. Roger*
Affiliation:
Liquides Ioniques et Interfaces Chargées LI2C-UPMC, 4 place Jussieu, 75252 Paris Cedex 05, France
J. N. Pons
Affiliation:
Liquides Ioniques et Interfaces Chargées LI2C-UPMC, 4 place Jussieu, 75252 Paris Cedex 05, France
R. Massart
Affiliation:
Liquides Ioniques et Interfaces Chargées LI2C-UPMC, 4 place Jussieu, 75252 Paris Cedex 05, France
A. Halbreich
Affiliation:
Laboratoire des Milieux Désordonnés et Hétérogènes UPMC, 4 place Jussieu, 75252 Paris Cedex 05, France
J. C. Bacri
Affiliation:
Laboratoire des Milieux Désordonnés et Hétérogènes UPMC, 4 place Jussieu, 75252 Paris Cedex 05, France
*
[email protected]
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Abstract

Ferrofluids are colloidal solutions of iron oxide magnetic nanoparticles in either a polar or no polar liquid. We present here two biological applications using maghemite (γFe2O3) ferrofluids: magnetic cell sorting and magnetocytolysis.The first application employs magnetic particles binding a biological effector, which is capable to recognize the target cells specifically. These cells become magnetic and can be sorted in a gradient of magnetic field. We describe first the various steps of the synthesis of a biocompatible ferrofluid and the grafting an effector protein onto the particles. We then describe the use of particles carrying annexin V in the separation and quantification of damaged erythrocytes in blood samples. This very sensitive technique can be used to follow the erythrocytes ageing of normal blood samples during their storage under blood bank conditions or to detect the membrane modifications that are associated with some pathologies such as malaria or Alzheimer's disease.The dependence of the magnetic susceptibility versus the frequency is a way to transform magnetic energy into thermal energy. Magnetocytolysis is the destruction of cells, carrying magnetic particles, through the action of an alternating magnetic field (about 1 MHz). We present here preliminary experiments with macrophages, which demonstrate the method's feasibility and the formation of the non-specific interactions between the cells and the magnetic particles.

Keywords

Type
Research Article
Information
The European Physical Journal - Applied Physics , Volume 5 , Issue 3 , March 1999 , pp. 321 - 325
Copyright
© EDP Sciences, 1999

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Footnotes

*

This paper was presented at "Journées Oxydes Magnétiques" held at Dijon the March 24-26, 1998.

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