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Transcriptional regulatory activity of the cereal grain bZip protein TaABF1 can be either stimulated or inhibited by phosphorylation

Published online by Cambridge University Press:  01 February 2019

Alison E. Smith ,
Xi Yang ,
Justin Lutian ,
David Chelimo ,
Juvenal Lopez ,
Grace Uwase  and
Russell R. Johnson Open the ORCID record for Russell R. Johnson [Opens in a new window]
Alison E. Smith
Affiliation:
Department of Biology, Colby College, 5723 Mayflower Hill, Waterville ME 04901, USA
Xi Yang
Affiliation:
Department of Biology, Colby College, 5723 Mayflower Hill, Waterville ME 04901, USA
Justin Lutian
Affiliation:
Department of Biology, Colby College, 5723 Mayflower Hill, Waterville ME 04901, USA
David Chelimo
Affiliation:
Department of Biology, Colby College, 5723 Mayflower Hill, Waterville ME 04901, USA
Juvenal Lopez
Affiliation:
Department of Biology, Colby College, 5723 Mayflower Hill, Waterville ME 04901, USA
Grace Uwase
Affiliation:
Department of Biology, Colby College, 5723 Mayflower Hill, Waterville ME 04901, USA
Russell R. Johnson*
Affiliation:
Department of Biology, Colby College, 5723 Mayflower Hill, Waterville ME 04901, USA
*
Author for correspondence: Russell R. Johnson, Email: [email protected]
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Abstract

The wheat bZip transcription factor TaABF1 mediates both abscisic acid (ABA)-induced and ABA-suppressed gene expression. As levels of TaABF1 protein do not change in response to ABA, and TaABF1 is in a phosphorylated state in vivo, we investigated whether TaABF1 could be regulated at the post-translational level. In bombarded aleurone cells, a TaABF1 protein carrying phosphomimetic mutations (serine to aspartate) at four sites (S36D, S37D, S113D, S115D) was three to five times more potent than wild-type TaABF1 in activating HVA1, an ABA-responsive gene. The phosphomimetic mutations also increased the ability of TaABF1 to downregulate the ABA-suppressed gene Amy32b. These findings strongly suggest that phosphorylation at these sites increases the transcriptional regulatory activity of TaABF1. In contrast to the activation observed by the quadruple serine to aspartate mutation, a single S113D mutation completely eliminated the ability of TaABF1 to upregulate HVA1 or downregulate Amy32b. Thus phosphorylation of TaABF1 can either stimulate or inhibit the activity of TaABF1 in regulating downstream genes, depending on the site and pattern of phosphorylation. Mutation of S318 and S322 (in the bZIP domain) eliminated the ability of TaABF1 to activate HVA1, but had no effect on the ability of TaABF1 to downregulate Amy32b, suggesting that TaABF1 represses Amy32b expression through a mechanism other than direct DNA binding. An important step towards understanding how ABA and gibberellin (GA) signals are integrated through TaABF1 phosphorylation to regulate downstream gene expression is to clarify the effects of those hormones on the expression of specific genes. In contrast to some other ABA-induced genes, we found that HVA1 induction by ABA or TaABF1 is not inhibited by GA.

Keywords

abscisic acidgerminationgibberellinimbibitionphosphorylationwheat
Type
Research Paper
Information
Seed Science Research , Volume 29 , Issue 1 , March 2019 , pp. 21 - 28
Copyright
Copyright © Cambridge University Press 2019 

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