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Tomato mutants sensitive to abiotic stress display different abscisic acid content and metabolism during germination

Published online by Cambridge University Press:  01 December 2008

Andrea Andrade ,
Oscar Masciarelli ,
Sergio Alemano ,
Virginia Luna  and
Guillermina Abdala
Andrea Andrade
Affiliation:
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800Río Cuarto, Argentina
Oscar Masciarelli
Affiliation:
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800Río Cuarto, Argentina
Sergio Alemano
Affiliation:
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800Río Cuarto, Argentina
Virginia Luna
Affiliation:
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800Río Cuarto, Argentina
Guillermina Abdala*
Affiliation:
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800Río Cuarto, Argentina
*
*Correspondence Fax: 0054-358-4676230 Email: [email protected]
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Abstract

We report the determination of abscisic acid (ABA) and its metabolites, phaseic acid (PA), dihydrophaseic acid (DPA) and ABA glucose ester (ABA-GE), in non-dormant dry and imbibed seeds of tomato (Solanum lycopersicum Mill.) cv. Moneymaker (wild type), and its tss1, tss2 and tos1 mutants. High ABA in dry seeds may originate from ABA accumulation in the sheath tissue, which was in contact with an ABA-containing medium, the endocarpus. The highest germination percentages at 72 h, observed in tss1 and tss2, coincided with minimal ABA content. Wild-type and mutant seeds showed different ABA and catabolic patterns, and these were correlated with their sensitivity to abiotic stress. Whereas dry seeds showed a high basal ABA, imbibed seeds showed higher ABA metabolite content, particularly DPA. The dramatic decrease of ABA following seed imbibition suggests an activation of ABA catabolism during the early stages of the germination process. The observed variation of ABA metabolites among dry and imbibed seeds of Solanum lycopersicum cv. Moneymaker and its tss1, tss2 and tos1 mutants shows that ABA metabolism is differentially regulated in these genotypes.

Keywords

abiotic stressabscisic acidabscisic acid metabolitesgerminationSolanum lycopersicumtomato mutants
Type
Research Article
Information
Seed Science Research , Volume 18 , Issue 4 , December 2008 , pp. 249 - 258
Copyright
Copyright © Cambridge University Press 2008

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