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Sucrose and raffinose contents and acquisition of desiccation tolerance in immature maize embryos

Published online by Cambridge University Press:  19 September 2008

A. Bochicchio ,
E. Rizzi ,
C. Balconi ,
P. Vernieri  and
C. Vazzana
A. Bochicchio*
Affiliation:
Dipartimento di Agronomia e Produzioni Erbacee, P. le Cascine 18, 50144 Firenze, Italy
E. Rizzi
Affiliation:
Istituto Sperimentale per la Cerealicoltura Sez. Maiscoltura, Via di Stezzano 24, 24100 Bergamo, Italy
C. Balconi
Affiliation:
Istituto Sperimentale per la Cerealicoltura Sez. Maiscoltura, Via di Stezzano 24, 24100 Bergamo, Italy
P. Vernieri
Affiliation:
Dipartimento di Biologia delle Piante Agrarie, Sezione di Orticoltura e Floricoltura, Viale delle Piagge 23, 56124 Pisa, Italy
C. Vazzana
Affiliation:
Dipartimento di Agronomia e Produzioni Erbacee, P. le Cascine 18, 50144 Firenze, Italy
*
* Correspondence
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Abstract

We report preliminary experiments to test the hypothesis that two sugars are involved in the acquisition of desiccation tolerance in immature maize embryos. Sucrose and raffinose were quantified during embryo development, at 16–27 days after pollination (DAP) and during slow dehydration. Embryos were exposed to slow drying either upon excision (18 and 16 DAP) or after an incubation treatment resulting in a decrease in sucrose (18 DAP). The sugars were separated by HPLC with a refractive-index detector; composition was determined by comparing peak areas of interest with those of individual sugar standards. Although further evidence is needed, our results do not appear to support the hypothesis.

Keywords

desiccation tolerancemaizeembryosucroseraffinoseZea mays
Type
Short Communication
Information
Seed Science Research , Volume 4 , Issue 2 , June 1994 , pp. 123 - 126
Copyright
Copyright © Cambridge University Press 1994

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