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Storage of short-lived seeds of Inga vera subsp. affinis in osmotic medium

Published online by Cambridge University Press:  27 July 2020

Larissa C. V. Pereira ,
Rafaella C. Mayrinck ,
Carolina R. Zambon ,
Anderson C. José  and
José M.R. Faria
Larissa C. V. Pereira
Affiliation:
Tree Seed Laboratory, Department of Forest Science, Federal University of Lavras, Brazil
Rafaella C. Mayrinck
Affiliation:
Mistik Askiwin Laboratory, Agriculture Building, University of Saskatchewan, Saskatoon, Canada
Carolina R. Zambon
Affiliation:
Natural Resources Institute, Federal University of Itajubá, Itajubá, Brazil
Anderson C. José
Affiliation:
Tree Seed Laboratory, Department of Forest Science, Federal University of Lavras, Brazil
José M.R. Faria*
Affiliation:
Tree Seed Laboratory, Department of Forest Science, Federal University of Lavras, Brazil
*
Author for correspondence: José M.R. Faria, E-mail: [email protected]
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Abstract

Inga vera subsp. affinis (Fabaceae) is a tree species native to riparian forests in Southeast Brazil and is key for the restoration of deforested areas. The species produce seeds that are highly recalcitrant. Extreme sensitivity to desiccation as well as vivipary are commonly observed in mature seeds, which also tend towards polyembryony. Past research has shown that typical strategies to store seeds are inapplicable to Inga vera as viability is completely lost when seeds are either dried to around 28% water content (wet basis) or stored at 5°C for a few weeks. Here, we examine the feasibility of storing the seeds under hydrated conditions but at reduced water potential. Freshly collected seeds were kept under conventional storage conditions (plastic bags in cold chamber, 5°C) and in polyethylene glycol (PEG) solutions (−1.6 and −2.4 MPa) at 10°C. Seed germination was assessed after various intervals of time, until all seeds had lost viability. Before storage, seeds attained 100% germination and produced an average of 1.8 normal seedlings per seed (due to polyembryony). Storage in PEG at −1.6 MPa maintained 90% germination (radicle protrusion) and one normal seedling per seed on average for more than 200 d. Osmotic storage likely slowed down metabolism within the seed and hence consumption of food reserves. The storage time achieved has practical applications for in situ restoration, but cannot address ex situ germplasm conservation. Extending shelf life for an additional 6 months allows tree nurseries to optimize the production of seedlings so that they can be planted during the wet season.

Keywords

germplasm conservationrecalcitrant seedseed conservationstorage capacity
Type
Research Paper
Information
Seed Science Research , Volume 30 , Issue 2 , June 2020 , pp. 156 - 160
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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References

Andreo, Y, Nakagawa, J and Barbedo, CJ (2006) Mobilização de água e conservação da viabilidade de embriões de sementes recalcitrantes de ingá (Inga vera Willd. subsp. affinis (DC.) TD Pennington). Revista Brasileira de Botânica 29, 309–318.Google Scholar
Berjak, P and Pammenter, NW (1994) Recalcitrant is not an all-or-nothing situation. Seed Science Research 4, 263–264.CrossRefGoogle Scholar
Berjak, P and Pammenter, NW (2013) Translating theory into practice for conservation of germplasm of recalcitrant-seeded species. Biotecnología Vegetal 13, 75–92.Google Scholar
Bilia, DAC and Barbedo, CJ (1997) Estudos de germinação e armazenamento de sementes de Inga uruguensis. Hook. et Arn. Científica 25, 379–391.Google Scholar
Bonjovani, MR and Barbedo, CJ (2008) Sementes recalcitrantes: intolerantes a baixas temperaturas? Embriões recalcitrantes de Inga vera Willd. subsp. affinis (DC.) T. D. Penn. toleram temperatura sub-zero. Revista Brasileira de Botânica 31, 345–356.Google Scholar
Bradford, KJ (1995) Water relations in seed germination, pp. 351–396in Kigel, J and Galili, G (Eds) Seed development and germination, New York, USA, Marcel Dekker Inc.Google Scholar
Brasil (2009) Regras para análise de sementes. Brasília, Ministério da Agricultura Pecuária e Abastecimento.Google Scholar
Caccere, RSP, Teixeira, SP, Centeno, DC, Figueiredo-Ribeiro, RCL and Braga, MR (2013) Metabolic and structural changes during early maturation of Inga vera seeds are consistent with the lack of a desiccation phase. Journal of Plant Physiology 170, 791–800.Google ScholarPubMed
Cardoso, RB, Binotti, FFS and Cardoso, ED (2012) Potencial fisiológico de sementes de crambe em função de embalagens e armazenamento. Pesquisa Agropecuária Tropical 42, 272–278.CrossRefGoogle Scholar
Carvalho, PER (1994) Espécies florestais brasileiras: recomendações silviculturais, potencialidades e uso da madeira. Brasília, Embrapa/CNPF.Google Scholar
Cruz Neto, O, Aguiar, AV, Twyford, AD, Neaves, LE, Pennington, RT and Lopes, AV (2014) Genetic and ecological outcomes of Inga vera subsp. affinis (Leguminosae) tree plantations in a fragmented tropical landscape. PLoS ONE 9, 1–8.Google Scholar
Cruz Neto, O, Machado, IC, Galetto, L and Lopes, AV (2015) The influence of nectar production and floral visitors on the female reproductive success of Inga (Fabaceae): a field experiment. Botanical Journal of the Linnean Society 29, 230–245.CrossRefGoogle Scholar
Davide, AC and Faria, JMR (2008) Viveiros florestais, pp. 83–122in Davide, AC and Silva, EAA (Eds) Produção de sementes e mudas de espécies florestais, Lavras, Editora UFLA.Google Scholar
FAO (2014) Genebank standards for plant genetic resources for food and agriculture. Rev. ed. Rome.Google Scholar
Faria, JM, van Lammeren, AAM and Hilhorst, HWM (2004) Desiccation sensitivity and cell cycle aspects in seeds of Inga vera subsp. affinis. Seed Science Research 14, 165–178.CrossRefGoogle Scholar
Faria, JM, Davide, LC, Silva, EAA, Davide, AC, Pereira, RC, van Lammeren, AAM and Hilhorst, HWM (2006) Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage. Brazilian Journal of Plant Physiology 18, 503–513.CrossRefGoogle Scholar
Ibrahim, AE, Roberts, EH and Murdoch, AJ (1983) Viability of lettuce seeds: II. Survival and oxygen uptake in osmotically controlled storage. Journal of Experimental Botany 34, 631–640.CrossRefGoogle Scholar
Karnovsky, MJ (1961) Simple methods for ‘staining with lead’ at high pH in electron microscopy. Journal of Biophysical and Biochemical Cytology 11, 729–732.Google Scholar
Kraus, JE and Arduin, M (1997) Manual básico de métodos em morfologia vegetal. Seropédica, Edur.Google Scholar
Leprince, O, Buitink, J, and Hoekstra, FA (1999) Axes and cotyledons of recalcitrant seeds of Castanea sativa Mill. exhibit contrasting responses of respiration to drying in relation to desiccation sensitivity. Journal of Experimental Botany 50, 1515–1524.Google Scholar
Marcos Filho, J (2005) Fisiologia de sementes de plantas cultivadas. Piracicaba, Fealq.Google Scholar
Marques, A, et al. (2019) A blueprint of seed desiccation sensitivity in the genome of Castanospermum australe. bioRxiv, 665661.Google Scholar
Pammenter, NW and Berjak, P (1994) Why do stored hydrated recalcitrant seeds die? Seed Science Research 4, 187–191.CrossRefGoogle Scholar
Pammenter, NW, Naidoo, S and Berjak, P (2003) Desiccation rate, desiccation response and damage accumulation: can desiccation sensitivity be quantified?, pp. 319–325in Nicolás, G, Bradford, KJ, Côme, D and Pritchard, HW (Eds) The biology of seeds: recent research advances, Wallingford, Oxon, CABI Publishing.Google Scholar
Parisi, JJ, Biagi, JD, Barbedo, CJ and Medina, PF (2013) Viability of Inga vera Willd. subsp. affinis (DC.) TD Penn. embryos according to the maturation stage, fungal incidence, chemical treatment and storage. Journal of Seed Science 35, 70–76.CrossRefGoogle Scholar
Pritchard, HW, Haye, AJ, Wright, WJ and Steadman, KJ (1995) A comparative study of seed viability in Inga species: desiccation tolerance in relation to the physical characteristics and chemical composition of the embryo. Seed Science and Technology 23, 85–100.Google Scholar
R Core Team (2013) R: a language and environment for statistical computing.Google Scholar
Roach, T, Beckett, RP, Minibayeva, FV, Colville, L, Whitaker, C, Chen, H, Bailly, C and Kranner, I (2010) Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds. Plant, Cell & Environment 33, 59–75.Google ScholarPubMed
Roberts, EH (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.Google Scholar
Stein, VC and Fu, Y (2007) Germination in vitro and ex vitro of Inga vera Willd. subsp. affinis (DC.) TD Penn. Ciência e Agrotecnologia 31, 1702–1708.CrossRefGoogle Scholar
Stein, VC, Paiva, R, Vargas, DP, Soares, FP, Alves, E and Nogueira, GF (2010) Ultrastructural calli analysis of Inga vera Willd. subsp. affinis (DC.) TD Penn. Revista Árvore 34, 789–796.CrossRefGoogle Scholar
Ventrella, MC and Almeida, AL (2013) Métodos histoquímicos aplicados às sementes. Viçosa, UFV.Google Scholar
Vertucci, CW (1989) The effects of low water contents on physiological activities of seeds. Physiologia Plantarum 77, 172–176.CrossRefGoogle Scholar
Vertucci, CW and Farrant, JM (1995) Acquisition and loss of desiccation tolerance, pp. 237–271in Kigel, J and Galili, G (Eds) Seed development and germination, New York, USA, Marcel Dekker Inc.Google Scholar
Walters, C, Pammenter, NW, Berjak, P and Crane, J (2001) Desiccation damage, accelerated ageing and respiration in desiccation tolerant and sensitive seeds. Seed Science Research 11, 135–148.Google Scholar