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The role of plant growth regulators in the development and germination of the desiccation-sensitive (recalcitrant) seeds of Avicennia marina

Published online by Cambridge University Press:  19 September 2008

Jill M. Farrant ,
Patricia Berjak ,
J. G. M. Cutting  and
N. W. Pammenter
Jill M. Farrant
Affiliation:
Plant Cell Biology Research Group, Department of Biology, University of Natal, King George V Ave., Durban, 4001South Africa
Patricia Berjak*
Affiliation:
Plant Cell Biology Research Group, Department of Biology, University of Natal, King George V Ave., Durban, 4001South Africa
J. G. M. Cutting
Affiliation:
Department of Horticultural Science, University of Natal, P.O. Box 375, Pietermaritzburg, 3200South Africa
N. W. Pammenter
Affiliation:
Plant Cell Biology Research Group, Department of Biology, University of Natal, King George V Ave., Durban, 4001South Africa
*
* Correspondence
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Abstract

The contents of the cytokinins zeatin (Z), zeatin riboside (ZR), and isopentenyladenine (IPA), the combined contents of gibberellins1 + 3 (GA), and the contents of indoleacetic acid (IAA) and abscisic acid (ABA) were measured during the development of the desiccation-sensitive seeds of Avicennia marina (Forssk.) Vierh. During the stage of histodifferentiation the amounts of these plant growth regulators (PGRs) were measured on whole fruits. During the phase of seed growth and reserve accumulation measurements were made on the embryonic axis, cotyledons and pericarp separately. Patterns in the amounts of PGRs present during histodifferentiation were similar to those reported for desiccation-tolerant seeds and it suggested that this process is under similar ‘hormonal’ control in A. marina as in orthodox seeds. Very high contents of cytokinins, particularly ZR, were present in both axes and cotyledons during reserve accumulation. This is thought to be related to the nature of the reserves accumulated (soluble sugars), rather than to the phenomenon of desiccation sensitivity. With the exception of ABA, embryonic contents of PGRs were relatively high at seed shedding, consistent with the rapid germination of this highly recalcitrant seed. ABA contents in the embryo were low during reserve accumulation, but concentrations in the pericarp increased throughout this development stage. ABA in the perticap could act to prevent precocious germination. The low concentrations of ABA in the embryo could be related to the desiccation-sensitivity of the seeds of A. marina.

Keywords

ABAAvicennia marinacytokininsdesiccation tolerance/sensitivityplant growth regulatorsrecalcitrantseed development
Type
Research Papers
Information
Seed Science Research , Volume 3 , Issue 1 , March 1993 , pp. 55 - 63
Copyright
Copyright © Cambridge University Press 1993

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Footnotes

3

Present address: Department of Botany, University of Cape Town, Private Bag, Rondebosch, 7700 South Africa

References

Badenoch-Jones, J., Letham, D.S., Parker, C.W. and Rolfe, B.G. (1984) Quantitation of cytokinins in biological samples using antibodies against zeatin riboside. Plant Physiology 75, 1117–1125.CrossRefGoogle ScholarPubMed
Bartels, D., Singh, M. and Salamini, F. (1988) Onset of desiccation tolerance during development of the barley embryo. Planta 175, 485–492.CrossRefGoogle ScholarPubMed
Berjak, P., Farrant, J.M. and Pammenter, N.W. (1989) The basis of recalcitrant seed behaviour, pp 89–108 in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.CrossRefGoogle Scholar
Bialek, K. and Cohen, J.D. (1989) Free and conjugated indole3-acetic acid in developing bean seeds. Plant Physiology 91, 775–779.CrossRefGoogle Scholar
Blackman, S., Wettlaufer, S.H., Obendorf, R.L. and Leopold, A.C. (1991) Maturation proteins associated with desiccation tolerance in soybean. Plant Physiology 96, 868–874.CrossRefGoogle ScholarPubMed
Blumenfeld, A. and Gazit, S. (1970) Cytokinin activity in avocado seeds during fruit development. Plant Physiology 46, 331–333.CrossRefGoogle ScholarPubMed
Bradbeer, J.W. (1968) Studies in seed dormancy. IV. The role of endogenous inhibitors and gibberellin in the dormancy and germination of Corylus avellana L. seeds. Planta 78, 266–276.CrossRefGoogle ScholarPubMed
Bradford, K.J. and Chandler, P.M. (1992) Expression of “dehydrin-like” proteins in embryos and seedlings of Zizania palustris and Oryza sativa during dehydration. Plant Physiology 99, 488–494.CrossRefGoogle ScholarPubMed
Brenner, M.L. (1987) The role of hormones in photosynthate partitioning and seed filling. pp 474–493 in Davies, P.J. (Ed.) Plant hormones and their role in plant growth and development. Dordrecht, Boston, London, Kluwer Academic Publishers.CrossRefGoogle Scholar
Chin, H.F. and Roberts, E.H. (1980) Recalcitrant crop seeds. Kuala Lumpur, Tropical Press, SDN.BHD.Google Scholar
Cionini, P.G., Bennici, A., Alpi, A., D'Amato, F. (1976) Suspensor gibberellin and in vitro development of Phaseolus coccineus embryos. Planta 131, 115–117.CrossRefGoogle ScholarPubMed
Cohen, J.D. and Bandurski, R.S. (1982) Chemistry and physiology of the bound auxins. Annual Review of Plant Physiology 33, 403–430.CrossRefGoogle Scholar
Cutting, J.G.M. and Bower, J.P. (1989) The relationship between basipetal auxin transport and calcium allocation in vegetative and reproductive flushes in avocado. Scientia Horticulturae 41, 27–34.CrossRefGoogle Scholar
Cutting, J.G.M., Hofman, P.J., Lishman, A.W. and Wolstenholme, B.N. (1986) Abscisic acid, isopentyladenine and isopentyl adenosine concentrations in ripening fruit as determined by radioimmunoassay. Acta Horticulturae 179, 793–800.CrossRefGoogle Scholar
Cutting, J.G.M., Lishman, A.W., van der Hoven, A. and Wolstenholme, B.N. (1983) Development of a radioimmunoassay for the cytokinin isopentyl adenosine. Crop Production 12, 133–135.Google Scholar
Davey, J.E. and van Staden, J. (1979) Cytokinins in Lupinus albus L. IV. Distribution in seeds. Plant Physiology 63, 873–877.CrossRefGoogle Scholar
Dodd, M.C. (1981) The biochemical and cytokinin changes in the developing and germinating seeds of Podocarpus henkelii Stapf. Ph.D. Thesis, University of Natal, Pietermaritzburg, South Africa.Google Scholar
Eeuwens, C.J. and Schwabe, W.W. (1975) Seed and pod wall development in Piston sativum L. in relation to extracted and applied hormones. Journal of Experimental Botany 26, 1–14.CrossRefGoogle Scholar
Farrant, J.M. (1992) Some aspects of the development of the homoiohydrous (recalcitrant) seeds of Avicennia marina (Forssk.) Vierh. Ph.D Thesis, University of Natal, Durban, South Africa.Google Scholar
Farrant, J.M., Berjak, P. and Pammenter, N.W. (1992 a) Proteins in development and germination of a desiccation sensitive (recalcitrant) seed species. Plant Growth Regulation 11, 257–265.CrossRefGoogle Scholar
Farrant, J.M., Berjak, P. and Pammenter, N.W. (1993) Studies in the development of the desiccation sensitive (recalcitrant) seeds of Avicennia marina (Forssk.) Vierh.: the acquisition of germinability and response to storage and desiccation. Annals of Botany. In press.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1988) Recalcitrance—a current assessment. Seed Science and Technology 16, 155–166.Google Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1989) Germination associated events and the desiccation sensitivity of recalictrant seeds—a study on three unrelated species. Planta 176, 189–198.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1992 b) Development of the recalcitrant (homoiohydrous) seeds of Avicennia marina: anatomical, ultrastructural and biochemical events associated with development from histodifferentiation to maturation. Annals of Botany 70, 75–86.CrossRefGoogle Scholar
Finch-Savage, W.E. (1992) Seed development in the recalcitrant species Quercus robur L.: germinability and desiccation tolerance. Seed Science Research 2, 17–22.CrossRefGoogle Scholar
Finch-Savage, W.E., Clay, H.A., Blake, P.S. and Browning, G. (1992) Seed development in the recalcitrant species Quercus robur L.: water status and endogenous abscisic acid levels. Journal of Experimental Botany 43, 671–679.CrossRefGoogle Scholar
Fincher, G.B. (1989) Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annual Review of Plant Physiology and Plant Molecular Biology 40, 305–346.CrossRefGoogle Scholar
Grange, R.I. and Finch-Savage, W.E. (1992) Embryo water status and development of the recalcitrant species Quercusrobur L.: determination of water relation parameters by pressure-volume analysis. Journal of Experimental Botany 43, 657–662.CrossRefGoogle Scholar
Higgins, T.J.V. and Jacobsen, J.V. (1978) The influence of plant hormones on selected aspects of cellular metabolism. pp 467–514 in Letham, D.S., Goodwin, P.B. and Higgins, T.J.V. (Eds) Phytohormones and related compounds: A comprehensive treatise. Vol. 1. The biochemistry of phytohormones and related compounds. Amsterdam, New York, Oxford, Elsevier Biomedical Press.Google Scholar
Hirasawa, E. (1989) Auxins induce α-amylase activity in pea cotyledons. Plant Physiology 91, 484–486.CrossRefGoogle ScholarPubMed
Hocart, C.H., Badenoch-Jones, J., Parker, C.W., Letham, D.S. and Summons, R.E. (1988) Cytokinins of dry Zea mays seed: quantification by radioimmunoassay and gas chromatography-mass spectrometry. Plant Growth Regulation 7, 179–196.CrossRefGoogle Scholar
Hocart, C.H., Letham, D.S. and Parker, C.W. (1990) Metabolism and translocation of exogenous zeatin riboside in germinating seeds and seedlings of Zea mays. Journal of Experimental Botany 41, 1517–1524.CrossRefGoogle Scholar
Hofman, P.J., Featonby-Smith, B.C. and van Staden, J. (1985) The development of ELISA and IRA (sic) for cytokinin estimation and their application to a study of lunar periodicity in Ecklonia maxima (Osbeck) Papenf. Journal of Plant Physiology 122, 455–466.CrossRefGoogle Scholar
Jones, R.L. and Stoddart, J.L. (1977) Gibberellins and seed germination. pp 77–109 in Khan, A. A. (Ed.) The physiology and biochemistry of seed dormancy and germination. Amsterdam, New York, Oxford, Elsevier Biomedical Press.Google Scholar
Karssen, C.M., Zagórski, S., Kepezyński, J. and Groot, S.P.C. (1989) Key role for endogenous gibberellins in the control of seed germination. Annals of Botany 63, 71–80.CrossRefGoogle Scholar
Kermode, A.R. (1990) Regulatory mechanisms involved in the transition from seed development to germination. Critical Reviews in Plant Science 9, 155–195.CrossRefGoogle Scholar
Khan, A.A. (1982) Gibberellins and seed development. pp 111–135 in Khan, A.A. (Ed.) The physiology and biochemistry of seed development, dormancy and germination. Amsterdam, New York, Oxford, Elsevier Biomedical Press.Google Scholar
King, R.W. (1976) Abscisic acid in developing wheat grains and its relationship to grain growth and maturation. Planta 132, 943–51.CrossRefGoogle ScholarPubMed
King, R.W. (1982) Abscisic acid in seed development. pp 157–181 in Khan, A.A. (Ed.) The physiology and biochemistry of seed development, dormancy and germination. Amsterdam, New York, Oxford, Elsevier Biomedical Press.Google Scholar
Lorenzi, R., Vernieri, P. and Ceccarelli, N. (1988) Cytokinins in endosperm of Sechium edule SW. seeds. Journal of Plant Physiology 133, 310–325.CrossRefGoogle Scholar
Mothes, K. and Engelbrecht, E. (1961) Kinetin induced directed transport of substances in excised leaves in the dark. Phytochemistry 1, 58–62.CrossRefGoogle Scholar
Pence, V.C. (1991) Abscisic acid in developing zygotic embryos of Theobroma cacao. Plant Physiology 95, 1291–1293.CrossRefGoogle ScholarPubMed
Pharis, R.P. and King, R.W. (1985) Gibberellins and reproductive development in seed plants. Annual Review of Plant Physiology 36, 517–568.CrossRefGoogle Scholar
Piatkowski, D., Schneider, K., Salamini, F. and Bartels, D. (1990) Characterization of five abscisic acid-responsive cDNA clones isolated from the desiccation-tolerant plant Craterostigma plantagineum and their relationship to other water-stress genes. Plant Physiology 94, 1682–1688.CrossRefGoogle ScholarPubMed
Quatrano, R.S. (1987) The role of hormones during seed development. pp 494–514 in Davies, P.J. (Ed.) Plant hormones and their role in plant growth and development. Dordrecht, Boston, London, Kluwer Academic Publishers.CrossRefGoogle Scholar
Radley, M. (1976) The development of wheat grain in relation to endogenous growth substances. Journal of Experimental Botany 27, 1009–1021.CrossRefGoogle Scholar
Reinecke, D.M. and Bandurski, R.S. (1987) Auxin biosynthesis and metabolism. pp 24–42 in Davies, P.J. (Ed.) Plant hormones and their role in plant growth and development. Dordrecht, Boston, London, Kluwer Academic Publishers.CrossRefGoogle Scholar
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.Google Scholar
Roberts, J.A. and Hooley, R. (1988) Plant growth regulators. Glasgow, Blackie and Son Ltd.CrossRefGoogle Scholar
Sandberg, G., Ernstsen, A. and Hamnede, M. (1987) Dynamics of indole-3-acetic acid and indole-3-ethanol during development and germination of Pinus sylvestris seeds. Physiologia Plantarum 71, 411–418.CrossRefGoogle Scholar
Steinke, T.D. and Naidoo, Y. (1991) Respiration and net photosynthesis of cotyledons during establishment and early growth of propagules of the mangrove Avicennia marina at three temperatures. South African Journal of Botany 57, 171–174.CrossRefGoogle Scholar
Tillberg, E. (1977) Indoleacetic acid levels in Phaseolus, Zea and Pincus (sic) during seed germination. Plant Physiology 60, 317–319.CrossRefGoogle Scholar
Valpuesta, V., Quesada, M.A., Sánchez-Roldán, C., Tigier, H.A., Heredia, A. and Bukovac, M.J. (1989) Changes in indole-3-acetic acid, indole-3-acetic oxidase and peroxidase isoenzymes in the seeds of developing peach fruits. Plant Growth Regulation 8, 255–261.CrossRefGoogle Scholar
van Staden, J., Davey, J.E. and Brown, N.A.C. (1982) Cytokinins in seed development and germination. pp 137–156 in Khan, A.A. (Ed.) The physiology and biochemistry of seed development, dormancy and germination. Amsterdam, New York, Oxford, Elsevier Biomedical Press.Google Scholar
Varga, A. and Bruinsma, J. (1976) Roles of seeds and auxins in tomato fruit growth. Zeitschrift für Pflanzenphysiologie 80, 95–104.CrossRefGoogle Scholar
Yeung, E.C. and Sussex, I.M. (1979) Embryogeny of Phaseolus coccineus: the suspensor and the growth of the embryo-proper in vitro. Zeitschrift für Pflanzenphysiologie 91, 423–433.CrossRefGoogle Scholar