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A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms

Published online by Cambridge University Press:  22 February 2007

N. W. Pammenter  and
Patricia Berjak
N. W. Pammenter*
Affiliation:
Plant Cell Biology Research Unit, School of Life and Environmental Sciences, University of Natal, Durban, 4041, South Africa
Patricia Berjak
Affiliation:
Plant Cell Biology Research Unit, School of Life and Environmental Sciences, University of Natal, Durban, 4041, South Africa
*
*Correspondence Email: [email protected]; [email protected]
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Abstract

A suite of mechanisms or processes that together have been implicated in the acquisition and maintenance of desiccation tolerance in orthodox seeds is discussed in the context of the behaviour of desiccation-sensitive seeds, and where appropriate, parallels are drawn with the situation in vegetative plant tissues that tolerate dehydration. Factors included are: physical characteristics of cells and intracellular constituents; insoluble reserve accumulation; intracellular de-differentiation; metabolic ‘switching off’; presence, and efficient operation, of antioxidant systems; accumulation of putatively protective substances including LEAs, sucrose and other oligosaccharides, as well as amphipathic molecules; the presence and role of oleosins; and the presence and operation of repair systems during rehydration. The variable response to dehydration shown by desiccation-sensitive seeds is considered in terms of the absence or incomplete expression of this suite of mechanisms or processes.

Three categories of damage are envisaged: (i) reduction in cell volume which can lead to mechanical damage; (ii) aqueous-based degradative processes, probably consequent upon deranged metabolism at intermediate water contents. This is termed ‘metabolism-induced damage’ and its extent will depend upon the metabolic rate and the rate of dehydration; and (iii) the removal of water intimately associated with macromolecular surfaces leading to denaturation: this is referred to as desiccation damage sensu stricto. The effects of drying rate and the maturity status of seeds are considered in relation to the responses to dehydration, leading to the conclusion that the concept of critical water contents on a species basis is inappropriate. Viewing seed postharvest physiology in terms of a continuum of behaviour is considered to be more realistic than attempting precise categorization.

Rapid dehydration of excised embryonic axes (or other explants) from desiccation-sensitive seeds permits retention of viability (in the short term) to water contents approaching the level of non-freezable water. This opens up the possibility of long-term conservation, by cryopreservation techniques, of the genetic resources of species producing non-orthodox seeds.

Keywords

dehydration ratedesiccation-sensitivitydesiccation-tolerancedevelopmental statusintermediateorthodoxrecalcitrantresurrection plantseed
Type
Invited Review
Information
Seed Science Research , Volume 9 , Issue 1 , January 1999 , pp. 13 - 37
Copyright
Copyright © Cambridge University Press 1999

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References

Arrigoni, O., De Gara, L., Tommasi, F. and Liso, R. (1992) Changes in the ascorbate system during seed development of Vicia faba L. Plant Physiology 99, 235–238.CrossRefGoogle ScholarPubMed
Asghar, R., Fenton, R.D., DeMason, D.A. and Close, T.J. (1994) Nuclear and cytoplasmic localization of maize embryo and aleurone dehydrin. Protoplasma 177, 87–94.CrossRefGoogle Scholar
Ashraf, M. and Bray, C.M. (1993) DNA synthesis in primed leek (Allium porrum L.) seeds and evidence for repair and replication. Seed Science Research 3, 15–23.CrossRefGoogle Scholar
Bagnoli, F., Capuana, M. and Racchi, M.L.Developmental changes of catalase and superoxide dismutase isoenzymes in zygotic and somatic embryos of horse chestnut. Australian Journal of Plant Physiology in press.Google Scholar
Bain, J. and Mercer, F.V. (1966) Subcellular organisation of the developing cotyledons of Pisum sativum L. Australian Journal of Biological Sciences 19, 49–67.CrossRefGoogle Scholar
Bartels, D., Schneider, K., Terstappen, G., Piatkowski, D. and Salamini, F. (1990) Molecular cloning of abscisic acid-modulated genes which are induced during desiccation of the resurrection plant Craterostigma plantagineum. Planta 181, 27–34.CrossRefGoogle ScholarPubMed
Berjak, P. (1996) The rôle of micro-organisms in deterioration during storage of recalcitrant and intermediate seeds. pp. 121–126in Ouédraogo, A.-S.; Poulsen, K.; Stubsgaard, I. (Eds) Intermediate/recalcitrant tropical forest seeds, Rome, IPGRI.Google Scholar
Berjak., P. and Pammenter, N.W. (1997) Progress in the understanding and manipulation of desiccationsensitive (recalcitrant) seeds. pp. 689–703in Ellis, R.M.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, Kluwer Academic Publishers.CrossRefGoogle Scholar
Berjak, P. and Villiers, T.A. (1972) Ageing in plant embryos II. Age-induced damage and its repair during early germination. New Phytologist 71, 135–144.CrossRefGoogle Scholar
Berjak, P., Dini, M. and Pammenter, N.W. (1984) Possible mechanisms underlying the differing dehydration responses in recalcitrant and orthodox seeds: desiccation-associated subcellular changes in propagules of Avicennia marina. Seed Science and Technology 12, 365–384.Google Scholar
Berjak, P., Farrant, J.M. and Pammenter, N.W. (1989) The basis of recalcitrant seed behaviour. pp. 89–108in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.CrossRefGoogle Scholar
Berjak, P., Farrant, J.M., Mycock, D.J. and Pammenter, N.W. (1990) Recalcitrant (homoiohydrous) seeds: the enigma of their desiccation sensitivity. Seed Science and Technology 18, 297–310.Google Scholar
Berjak, P., Pammenter, N.W. and Vertucci, C.W. (1992) Homoiohydrous (recalcitrant) seeds: developmental status, desiccation sensitivity and the state of water in axes of Landolphia kirkii Dyer. Planta 186, 249–261.CrossRefGoogle ScholarPubMed
Berjak, P., Vertucci, C.W. and Pammenter, N.W. (1993) Effects of developmental status and dehydration rate on characteristics of water and desiccation-sensitivity in recalcitrant seeds of Camellia sinensis. Seed Science Research 3, 155–166.CrossRefGoogle Scholar
Berjak, P., Bradford, K.J., Kovach, D.A. and Pammenter, N.W. (1994) Differential effects of temperature on ultrastructural responses to dehydration in seeds of Zizania palustris. Seed Science Research 4, 111–121.CrossRefGoogle Scholar
Berjak, P., Campbell, G.K., Farrant, J.M., Omondi-Oloo, W. and Pammenter, N.W. (1995) Responses of seeds of Azadirachta indica (neem) to short-term storage under ambient or chilled conditions. Seed Science and Technology 23, 779–792.Google Scholar
Berjak, P., Mycock, D.J., Wesley-Smith, J., Dumet, D. and Watt, M.P. (1996) Strategies for in vitro conservation of hydrated germplasm. pp. 19–52in Normah, M.N.; Narimah, M.K.; Clyde, M.M. (Eds) In vitro conservation of plant genetic resources. Kuala Lumpur, Malaysia, Percetakan Watan Sdn.Bhd.Google Scholar
Bewley, J.D. (1979) Physiological aspects of desiccation tolerance. Annual Review of Plant Physiology 30, 195–238.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1994) Seeds. Physiology of development and germination. (2nd edition) New York, Plenum Press.CrossRefGoogle Scholar
Bewley, J.D. and Oliver, M.J. (1992) Desiccation tolerance in vegetative plant tissues and seeds: protein synthesis in relation to desiccation and a potential role for protection and repair mechanisms. pp. 141–160in Osmond, C.B.; Somero, G. (Eds) Water and life: a comparative analysis of water relationships at the organismic, cellular and molecular levels. Berlin, Springer Verlag.CrossRefGoogle Scholar
Bianchi, G., Gamba, A., Murelli, C., Salamini, F. and Bartels, D. (1992) Low molecular weight solutes in desiccated and ABA-treated calli of Craterostigma plantagineum. Phytochemistry 31, 1917–1922.CrossRefGoogle Scholar
Bianchi, G., Gamba, A., Limiroli, R., Pozzi, N., Elster, R., Salamini, F. and Bartels, D. (1993) The unusual sugar composition in leaves of the resurrection plant Myrothamnus flabellifolia. Physiologia Plantarum 87, 223–226.CrossRefGoogle Scholar
Blackman, S.A., Wettlaufer, S.H., Obendorf, R.L. and Leopold, A.C. (1991) Maturation proteins associated with desiccation tolerance in soybean. Plant Physiology 96, 868–874.CrossRefGoogle ScholarPubMed
Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1992) Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiology 100, 225–230.CrossRefGoogle ScholarPubMed
Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1995) Desiccation tolerance in developing soybean seeds: the role of stress proteins. Physiologia Plantarum 93, 630–638.CrossRefGoogle Scholar
Boubriak, I., Kargiolaki, H., Lyne, L. and Osborne, D.J. (1997) The requirement for DNA repair in desiccation tolerance of germinating embryos. Seed Science Research 7, 97–105.CrossRefGoogle Scholar
Bradford, K.J. and Chandler, P.M. (1992) Expression of dehydrin-like proteins in embryos and seedlings of Zizania palustris and Oryza sativa during dehydration. Plant Physiology 99, 488–494.CrossRefGoogle ScholarPubMed
Bray, C.M. (1995) Biochemical processes during the osmopriming of seeds. pp. 767–789in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker Inc.Google Scholar
Bray, E. (1993) Molecular responses to water deficit. Plant Physiology 103, 1035–1040.CrossRefGoogle ScholarPubMed
Bray, C.M., Ashraf, M., Davison, P.A. and Taylor, R.M. (1993) Molecular markers of seed quality. pp. 887–896in Côme, D.; Corbineau, F. (Eds) Fourth International Workshop on seeds: Basic and applied aspects of seed biology. Paris, ASFIS.Google Scholar
Bruni, F. and Leopold, A.C. (1992) Cytoplasmic glass formation in maize embryos. Seed Science Research 2, 251–253.CrossRefGoogle Scholar
Brunori, A. (1967) A relationship between DNA synthesis and water content during ripening of Vicia faba seeds. Caryologia 20, 333–338.CrossRefGoogle Scholar
Buitink, J., Walters-Vertucci, C., Hoekstra, F.A. and Leprince, O. (1996) Calorimetric properties of dehydrating pollen. Plant Physiology 111, 235–242.CrossRefGoogle ScholarPubMed
Chaitanya, K.S.K. and Naithani, S.C. (1994) Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn. New Phytologist 126, 623–627.CrossRefGoogle Scholar
Chaudhury, R., Radhamani, J. and Chandel, K.P.S. (1991) Preliminary observations on the cryopreservation of desiccated embryonic axes of tea (Camelia sinensis (L.) O. Kuntze) seeds for genetic conservation. Cryo-Letters 12, 31–36.Google Scholar
Chen, Y. and Burris, J.S. (1990) Role of carbohydrates in desiccation tolerance and membrane behavior in maturing maize seed. Crop Science 30, 971–975.CrossRefGoogle Scholar
Chiatante, D., Brusa, P., Levi, M. and Sparvoli, E. (1991) Nuclear proteins during the onset of cell proliferation in pea root meristems. Journal of Experimental Botany 42, 45–50.CrossRefGoogle Scholar
Chien, C.-T. and Lin, T.-P.. (1997) Effect of harvest date on the storability of desiccation-sensitive seeds of Machilus kusanoi Hay. Seed Science and Technology 25, 361–371.Google Scholar
Chin, H.F. and Roberts, E.H. (1980) Recalcitrant crop seeds. Kuala Lumpur, Malaysia, Tropical Press SDN.BDH.Google Scholar
Clegg, J.S. (1986) The physical properties and metabolic status of Artemia cysts at low water content: the ‘Water Replacement Hypothesis’. pp. 169–187in Leopold, A.C. (Ed.) Membranes, metabolism and dry organisms. IthacaN.Y., Cornell University Press.Google Scholar
Close, T.J. (1997) Dehydrins: a commonality in the response of plants to dehydration and low temperature. Physiologia Plantarum 100, 291–296.CrossRefGoogle Scholar
Close, T.J., Kortt, A.A. and Chandler, P.M. (1989) A cDNAbased comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Molecular Biology 13, 95–108.CrossRefGoogle ScholarPubMed
Close, T.J., Fenton, R.D., Yang, A., Asghar, R., DeMason, D.A., Crone, D.E., Meyer, N.C. and Moonan, F. (1993) Dehydrin: the protein. in Close, T.J.; Bray, E.A. (Eds) Current topics in plant physiology, Vol. 10. Plant responses to cellular dehydration during environmental stress. Rockville, American Society of Plant Physiologists.Google Scholar
Côme, D. and Corbineau, F. (1996) Metabolic damage related to desiccation sensitivity. pp. 83–97in Ouédraogo, A.-S.; Poulsen, K.; Stubsgaard, F. (Eds) Intermediate/recalcitrant tropical forest tree seeds. Rome, IPGRI.Google Scholar
Crévecoeur, M., Deltour, R. and Bronchart, R. (1976) Cytological study on water stress during germination of Zea mays. Planta 132, 31–41.CrossRefGoogle Scholar
Crowe, J.H., Crowe, L.M., Carpenter, J.F. and Wistrom, C.A. (1987) Stabilization of dry phospholipid bilayers and proteins by sugars. Biochemical Journal 242, 1–10.CrossRefGoogle ScholarPubMed
Crowe, J.H., Hoekstra, F.A. and Crowe, L.M. (1992) Anhydrobiosis. Annual Review of Physiology 54, 579–599.CrossRefGoogle ScholarPubMed
Dasgupta, J., Bewley, J.D. and Yeung, E.C. (1982) Desiccation-tolerant and desiccation-intolerant stages during the development and germination of Phaseolus vulgaris seeds. Journal of Experimental Botany 33, 1045–1057.CrossRefGoogle Scholar
Deltour, R. (1985) Nuclear reactivation during early germination of the higher plant embryo. Journal of Cell Science 75, 43–83.CrossRefGoogle Scholar
Drennan, P.M., Smith, M.T., Goldsworthy, D. and van Staden, J. (1993) The occurrence of trehalose in the leaves of the desiccation-tolerant angiosperm Myrothamnus flabellifolia. Journal of Plant Physiology 142, 493–496.CrossRefGoogle Scholar
Dure, L. III. (1993) A repeating 11-mer amino acid motif and plant desiccation. Plant Journal 3, 363–369.CrossRefGoogle ScholarPubMed
Dure, L. III, Crouch, M., Harada, J., Ho, T.H.D., Mundy, J., Quatrano, R., Thomas, T. and Sung, Z.R. (1989) Common amino acid sequence domains among the LEA proteins of higher plants. Plant Molecular Biology 12, 475–486.CrossRefGoogle ScholarPubMed
Edwards, C.A. and Mumford, P.M. (1985) Factors affecting the oxygen consumption of sour orange (Citrus aurantium L.) seeds during imbibed storage and germination. Seed Science and Technology 13, 201–212.Google Scholar
Elder, R.H., Dell'Aquila, A., Mezzina, M., Sarasin, A. and Osborne, D.J. (1987) DNA ligase in repair and replication in the embryos of rye, Secale cereale. Mutation Research 181, 61–71.CrossRefGoogle Scholar
Ellis, R.H. and Roberts, E.H. (1980) Improved equations for the prediction of seed longevity. Annals of Botany 45, 13–30.CrossRefGoogle Scholar
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1990) An intermediate category of seed storage behaviour? I. Coffee. Journal of Experimental Botany 41, 1167–1174.CrossRefGoogle Scholar
Farrant, J.M. and Sherwin, H.W. (1998) Mechanisms of desiccation tolerance in seeds and resurrection plants. in Taylor, A.G.; Roos, E.; Huang, X.-L.. (Eds) Progress in seed research. Ithaca, Cornell University Press.Google Scholar
Farrant, J.M., Berjak, P. and Pammenter, N.W. (1985) The effect of drying rate on viability retention of recalcitrant propagules of Avicennia marina. South African Journal of Botany 51, 432–438.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1986) The increasing desiccation sensitivity of recalcitrant Avicennia marina seeds with storage time. Physiologia Plantarum 67, 291–298.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1988) Recalcitrance - a current assessment. Seed Science and Technology 16, 155–166.Google Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1989) Germination-associated events and the desiccation sensitivity of recalcitrant seeds - a study on three unrelated species. Planta 178, 189–198.CrossRefGoogle Scholar
Farrant, J.M., Berjak, P. and Pammenter, N.W. (1992a) Proteins in development and germination of a desiccation sensitive (recalcitrant) seed species. Plant Growth Regulation 11, 257–265.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1992b) Development of the recalcitrant (homoiohydrous) seeds of Avicennia marina: Anatomical, ultrastructural and biochemical events associated with development from histodifferentiation to maturation. Annals of Botany 70, 75–86.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1993a) Seed development in relation to desiccation tolerance: A comparison between desiccation-sensitive (recalcitrant) seeds of Avicennia marina and desiccation-tolerant types. Seed Science Research 3, 1–13.CrossRefGoogle Scholar
Farrant, J.M., Berjak, P., Cutting, J.G.M. and Pammenter, N.W. (1993b) The role of plant growth regulators in the development and germination of the desiccationsensitive seeds of Avicennia marina. Seed Science Research 3, 55–63.CrossRefGoogle Scholar
Farrant, J.M., Berjak, P. and Pammenter, N.W. (1993c) Studies on the desiccation-sensitive (recalcitrant) seeds of Avicennia marina (Forssk.) Vierh.: the acquisition of germinability and response to storage and desiccation. Annals of Botany 71, 405–410.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W., Berjak, P., Farnsworth, E.J. and Vertucci, C.W. (1996) Presence of dehydrin-like proteins and levels of abscisic acid in recalcitrant (desiccation sensitive) seeds may be related to habitat. Seed Science Research 6, 175–182.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W., Berjak, P. and Walters, C. (1997) Subcellular organization and metabolic activity during the development of seeds that attain different levels of desiccation tolerance. Seed Science Research 7, 135–144.CrossRefGoogle Scholar
Finch-Savage, W.E. (1992a) Seed water status and survival in the recalcitrant species Quercus robur L.: Evidence for a critical moisture content. Journal of Experimental Botany 43, 671–679.CrossRefGoogle Scholar
Finch-Savage, W.E. (1992b) Seed development in the recalcitrant species Quercus robur L.: Development of germinability and desiccation tolerance. Seed Science Research 2, 17–22.CrossRefGoogle Scholar
Finch-Savage, W.E. (1996) The role of developmental studies in research on recalcitrant and intermediate seeds. pp. 83–97in Ouédraogo, A.-S.; Poulsen, K.; Stubsgaard, F. (Eds) Intermediate/recalcitrant tropical forest tree seeds. Rome, IPGRI.Google Scholar
Finch-Savage, W.E. and Blake, P.S. (1994) Indeterminate development in desiccation-sensitive seeds of Quercus robur L. Seed Science Research 4, 127–133.CrossRefGoogle Scholar
Finch-Savage, W.E. and Clay, H.A. (1994) Water relations of germination in the recalcitrant seeds of Quercus robur L. Seed Science Research 4, 315–322.CrossRefGoogle Scholar
Finch-Savage, W.E. and Farrant, J.M. (1997) The development of desiccation sensitive seeds in Quercus robur L.: Reserve accumulation and plant growth regulators. Seed Science Research 7, 35–39.CrossRefGoogle Scholar
Finch-Savage, W.E., Grange, R.I., Hendry, G.A.F. and Atherton, N.M. (1993) Embryo water status and loss of viability during desiccation in the recalcitrant seed species Quercus robur L. pp. 723–730in Côme, D.; Corbineau, F. (Eds) Fourth international workshop on seeds: Basic and applied aspects of seed biology. ASFIS, Paris.Google Scholar
Finch-Savage, W.E., Hendry, G.A.F. and Atherton, N.M. (1994a) Free radical activity and loss of viability during drying of desiccation-sensitive tree seeds. Proceedings of the Royal Society of Edinburgh 102B, 257–260.Google Scholar
Finch-Savage, W.E., Pramanik, S.K. and Bewley, J.D. (1994b) The expression of dehydrin proteins in desiccation-sensitive (recalcitrant) seeds of temperate trees. Planta 193, 478–485.CrossRefGoogle Scholar
Finch-Savage, W.E., Blake, P.S. and Clay, H.A. (1996) Desiccation stress in recalcitrant Quercus robur L. seeds results in lipid peroxidation and increased synthesis of jasmonates and abscisic acid. Journal of Experimental Botany 47, 661–667.CrossRefGoogle Scholar
Finch-Savage, W.E., Bergervoet, J.H.W., Bino, R.J., Clay, H.A. and Groot, S.P.C. (1998) Nuclear replication activity during seed development, dormancy breakage and germination in three tree species: Norway maple (Acer platanoides L.), sycamore (Acer pseudoplatanus L.) and cherry (Prunus avium L.). Annals of Botany 81, 519–526.CrossRefGoogle Scholar
Fu, J.R., Zhang, B.Z., Wang, X.P., Qiao, Y.Z. and Huang, X.L. (1990) Physiological studies on desiccation, wet storage and cryopreservation of recalcitrant seeds of three fruit species and their excised embryonic axes. Seed Science and Technology 18, 743–754.Google Scholar
Fu, J.R., Jin, J.P., Peng, Y.F. and Xia, Q.H. (1994) Desiccation tolerance in two species with recalcitrant seeds: Clausena lansium (Lour.) and Litchi chinensis (Sonn.). Seed Science Research 4, 257–261.CrossRefGoogle Scholar
Gaff, D.F. (1989) Responses of desiccation tolerant ‘resurrection’ plants to water stress. pp. 264–311in Kreeb, K.H.; Richter, H.; Hinchley, T.M. (Eds) Structural and functional responses to environmental stresses: water shortages. The Hague, SPB Academic Publishing.Google Scholar
Galau, G.A. and Hughes, D.W. (1987) Coordinate accumulation of homologous transcripts of seven lea gene families during embryogenesis and germination. Developmental Biology 123, 213–221.CrossRefGoogle Scholar
Galau, G.A., Hughes, D.W. and Dure, L. (1986) Abscisic acid induction of cloned cotton late embryogenesisabundant (lea) mRNAs. Plant Molecular Biology 7, 157–170.CrossRefGoogle ScholarPubMed
Galau, G.A., Bijaisoradat, N. and Hughes, D.W. (1987) Accumulation kinetics of cotton late embryogenesisabundant mRNAs: coordinate regulation during embryogenesis and the role of abscisic acid. Developmental Biology 123, 198–212.CrossRefGoogle ScholarPubMed
Galau, G.A., Jakobsen, K.S. and Hughes, D.W. (1991) The controls of late dicot embryogenesis and early germination. Physiologia Plantarum 81, 280–288.CrossRefGoogle Scholar
Gee, O.H., Probert, R.J. and Coomber, S.A. (1994) ‘Dehydrin-like’ proteins and desiccation tolerance in seeds. Seed Science Research 4, 135–141.CrossRefGoogle Scholar
Goday, A., Albà, M.M., Torrent, M. and Pagès, M. (1993) Immunolocalization of RAB-17 (LEA D-11 Family) in maize embryo cells. p. 267in Close, T.J.; Bray, E.A. (Eds) Responses of plants to cellular dehydration during environmental stress. Rockville, MD, American Society of Plant Physiologists.Google Scholar
Golovina, E.A., Hoekstra, F.A. and Hemminga, M.A.Drying increases intracellular partioning of amphiphilic substances into the lipid phase: impact on membrane permeability and significance for desiccation tolerance. Plant Physiology 118, 975–986.CrossRefGoogle Scholar
Hallam, N.D. (1972) Embryogenesis and germination in rye (Secale cereale). I. Fine structure of the developing embryo. Planta 104, 157–166.CrossRefGoogle ScholarPubMed
Hallam, N.D. and Luff, S.E. (1980) Fine structural changes in the mesophyll tissue of the leaves of Xerophyta villosa during desiccation. Botanical Gazette 141, 173–179.CrossRefGoogle Scholar
Han, B., Berjak, P., Pammenter, N.W., Farrant, J.M. and Kermode, A.R. (1997) The recalcitrant plant species, Castanospermum australe and Trichilia dregeana, differ in their ability to produce dehydrin-related polypeptides during seed maturation and in response to ABA or water-deficit-related stresses. Journal of Experimental Botany 48, 1717–1726.CrossRefGoogle Scholar
Harada, J.J. (1997) Seed Maturation and Control of Germination. pp. 545–592in Larkins, B.A.; Vasil, I.K. (Eds) Cellular and molecular biology of plant seed development. Dordrecht, Kluwer Academic Publishers.CrossRefGoogle Scholar
Hendry, G.A.F. (1993) Oxygen, free radical processes and seed longevity. Seed Science Research 3, 141–153.CrossRefGoogle Scholar
Hendry, G.A.F., Finch-Savage, W.E., Thorpe, P.C., Atherton, N.M., Buckland, S.M., Nilsson, K.A. and Seel, W.A. (1992) Free radical processes and loss of viability during desiccation in the recalcitrant species Quercus robur L. New Phytologist 122, 273–279.CrossRefGoogle ScholarPubMed
Hirano, T., Mitchison, T.J. and Swedlow, J.R. (1995) The SMC family: from chromosome condensation to dosage compensation. Current Opinion in Cell Biology 7, 329–336.CrossRefGoogle ScholarPubMed
Hoekstra, F.A. and van Roekel, T. (1988) Desiccation tolerance of Papaver dubium L. pollen during its development in the anther: possible role of phospholipid and sucrose content. Plant Physiology 88, 626–632.CrossRefGoogle ScholarPubMed
Hoekstra, F.A., Crowe, J.H. and Crowe, L.M. (1991) Effect of sucrose on phase behavior of membranes in intact pollen of Typha latifolia L., as measured with Fourier transform infrared spectroscopy. Plant Physiology 97, 1073–1079.CrossRefGoogle ScholarPubMed
Hoekstra, F.A., Crowe, J.H. and Crowe, L.M. (1992) Germination and ion leakage are linked with phase transitions of membrane lipids during imbibition of Typha latifolia pollen. Physiologia Plantarum 84, 29–34.CrossRefGoogle Scholar
Hoekstra, F.A., Wolkers, W. F., Buitink, J., Golovina, E.A., Crowe, J.H. and Crowe, L.M. (1997) Membrane stabilization in the dry state. Comparative Biochemistry and Physiology 117A, 335–341.CrossRefGoogle Scholar
Holliday, R. (1989) Untwisting B-Z DNA. Trends in Genetics 5, 355–356.CrossRefGoogle ScholarPubMed
Hong, T.D. and Ellis, R.H. (1990) A comparison of maturation drying, germination, and desiccation tolerance between developing seeds of Acer psuedoplatanus L. and Acer platenoides L. New Phytologist 116, 589–596.CrossRefGoogle Scholar
Hong, T.D. and Ellis, R.H. (1996) A protocol to determine seed storage behaviour. Rome, IPGRI.Google Scholar
Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: Dependence on flatulenceproducing oligosaccharides and cyclitols - review and survey. Seed Science Research 4, 385–405.CrossRefGoogle Scholar
Hrazdina, G. and Jensen, R.A. (1992) Spatial organization of enzymes in plant metabolic pathways. Annual Review of Plant Physiology and Molecular Biology 43, 241–267.CrossRefGoogle Scholar
Huang, A.H.C. (1992) Oil bodies and oleosins in seeds. Annual Review of Plant Physiology and Molecular Biology 43, 177–200.CrossRefGoogle Scholar
Ibrahim, A.E., Roberts, E.H. and Murdoch, A.J. (1983) Viability of lettuce seeds. II. Survival and oxygen uptake in osmotically controlled storage. Journal of Experimental Botany 34, 631–640.CrossRefGoogle Scholar
Iljin, W.S. (1957) Drought resistance in plants and physiological processes. Annual Review of Plant Physiology 3, 341–363.Google Scholar
Ingram, I. and Bartels, D. (1996) The molecular basis of dehydration tolerance in plants. Annual Review of Plant Physiology and Plant Molecular Biology 47, 377–403.CrossRefGoogle ScholarPubMed
Ivanov, P.V. and Zlatanova, J.S. (1989) Quantitative changes in the histone content of the cytoplasm and the nucleus of germinating maize embryo cells. Plant Physiology and Biochemistry 27, 925–930.Google Scholar
Kermode, A.R. (1990) Regulatory mechanisms involved in the transition from seed development to germination. Critical Reviews in Plant Science 9, 155–195.CrossRefGoogle Scholar
Kermode, A.R. (1995) Regulatory mechanisms in the transition from seed development to germination: interactions between the embryo and the seed environment. pp. 273–3321in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker Inc.Google Scholar
Kermode, A.R. (1997) Approaches to elucidate the basis of desiccation-tolerance in seeds. Seed Science Research 7, 75–95.CrossRefGoogle Scholar
Kester, S.T., Geneve, R.L. and Houtz, R.L. (1997) Priming and accelerated aging affect L-isoaspartyl methyltransferase activity in tomato (Lycopersicon esculentum Mill.) seed. Journal of Experimental Botany 48, 943–949.CrossRefGoogle Scholar
King, M.W. and Roberts, E.H. (1980) Maintenance of recalcitrant seeds in storage. pp. 53–89in Chin, H.F..; Roberts, E.H. (Eds) Recalcitrant crop seeds. Kuala Lumpur, Tropical Press SDN.BDH.Google Scholar
King, M.W. and Roberts, E.H. (1982) The imbibed storage of cocoa (Theobroma cacao) seeds. Seed Science and Technology 10, 535–540.Google Scholar
Kioko, J., Berjak, P., Pammenter, N.W., Watt, P.M. and Wesley-Smith, J. (1998) Desiccation and cryopreservation of embryonic axes of Trichilia dregeana Sond. Cryo-Letters 19, 15–26.Google Scholar
Klein, S. and Pollock, B.M. (1968) Cell fine structure of developing lima bean seeds related to seed desiccation. American Journal of Botany 55, 658–672.CrossRefGoogle Scholar
Koster, K.L. (1991) Glass formation and desiccation tolerance in seeds. Plant Physiology 96, 302–304.CrossRefGoogle ScholarPubMed
Koster, K.L. and Leopold, A.C. (1988) Sugars and desiccation tolerance in seeds. Plant Physiology 88, 829–832.CrossRefGoogle ScholarPubMed
Kovach, D.A. and Bradford, K.J. (1992a) Imbibitional damage and desiccation tolerance of wild rice (Zizania palustris) seeds. Journal of Experimental Botany 43, 747–757.CrossRefGoogle Scholar
Kovach, D.A. and Bradford, K.J. (1992b) Temperature dependence of viability and dormancy of Zizania palustris var. interior seeds stored at high moisture contents. Annals of Botany 69, 297–301.CrossRefGoogle Scholar
Kuang, J., Gaff, D.F., Gianello, R.D., Blomstedt, C.K., Neale, A.D. and Hamill, J.D. (1995) Changes in in vivo protein complements in drying leaves of the desiccationtolerant grass Sporobolis stapfianus and the desiccationsensitive grass Sporobolis pyrimidalis. Australian Journal of Plant Physiology 22, 1027–1034.Google Scholar
Leopold, A.C. and Vertucci, C.W. (1986) Physical attributes of desiccated seeds. pp. 22–34in Leopold, A.C. (Ed.) Membranes, metabolism and dry organisms. Ithaca, London, Comstock.Google Scholar
Leopold, A.C., Sun, W.Q. and Bernal-Lugo, I. (1994) The glassy state in seeds: analysis and function. Seed Science Research 4, 267–274.CrossRefGoogle Scholar
Leprince, O. and Walters-Vertucci, C. (1995) A calorimetric study of the glass transitions behaviors in axes of bean seeds with relevance to storage stability. Plant Physiology 109, 1471–1481.CrossRefGoogle ScholarPubMed
Leprince, O., Bronchart, R. and Deltour, R. (1990a) Changes in starch and soluble sugars in relation to the acquisition of desiccation tolerance during maturation of Brassica campestris seeds. Plant Cell and Environment 13, 539–546.CrossRefGoogle Scholar
Leprince, O., Deltour, R., Thorpe, P.C., Atherton, N.M. and Hendry, G.A.F. (1990b) The role of free radicals and radical processing systems in loss of desiccation tolerance in germinating maize. New Phytologist 116, 573–580.CrossRefGoogle Scholar
Leprince, O., van der Werf, A., Deltour, R. and Lambers, H. (1992) Respiratory pathways in germinating maize radicles correlated with desiccation tolerance and soluble sugars. Physiologia Plantarum 85, 581–588.CrossRefGoogle Scholar
Leprince, O., Hendry, G.A.F. and McKersie, B.D. (1993) The mechanisms of desiccation tolerance in developing seeds. Seed Science Research 3, 231–246.CrossRefGoogle Scholar
Leprince, O., Colson, P., Houssier, C. and Deltour, R. (1995) Changes in chromatin structure associated with germination of maize and their relation with desiccation tolerance. Plant, Cell and Environment 18, 619–629.CrossRefGoogle Scholar
Leprince, O., van Aelst, A., Pritchard, H.W. and Murphy, D.J. (1998) Oleosins prevent oil-body coalescence during seed imbibition as suggested by a low-temperature scanning electron microscope study of desiccationtolerant and -sensitive oilseeds. Planta 204, 109–119.CrossRefGoogle Scholar
Lin, T.-P. and Chen, M.-H.. (1995) Biochemical characteristics associated with the development of the desiccationsensitive seeds of Machilus thunbergii Sieb. & Zucc. Annals of Botany 76, 381–387.CrossRefGoogle Scholar
Lin, T.-P. and Huang, N.-H.. (1994) The relationship between carbohydrate composition of some tree seeds and their longevity. Journal of Experimental Botany 45, 1289–1294.CrossRefGoogle Scholar
Long, S.R., Dale, R.M.K. and Sussex, I.M. (1981) Maturation and germination of Phaseolus vulgaris embryonic axes in culture. Planta 153, 405–415.CrossRefGoogle ScholarPubMed
Masters, C. (1984) Interactions between glycolytic enzymes and components of the cytomatrix. Journal of Cell Biology 99, 222s–225s.CrossRefGoogle ScholarPubMed
McKersie, B.D. (1991) The role of oxygen free radicals in mediating freezing and desiccation stress in plants. pp. 107–118in Pell, E..; Staffen, K. (Eds) Active oxygen and oxidative stress in plant metabolism. Current Topics in Plant Physiology, American Society of Plant Physiologists Series, Vol. 8.Google Scholar
McNulty, K. and Saunders, M.J. (1992) Purification and immunological detection of pea nuclear intermediate filaments: evidence for plant nuclear lamins. Journal of Cell Science 103, 407–414.CrossRefGoogle ScholarPubMed
Minguez, A. and Moreno Díaz de la Espina, S. (1993) Immunological characterization of the lamins in the nuclear matrix of onion. Journal of Cell Science 106, 431–439.CrossRefGoogle ScholarPubMed
Motete, N., Pammenter, N.W., Berjak, P. and Frédéric, J.C. (1997) Responses of the recalcitrant seeds of Avicennia marina to hydrated storage: events occurring at the root primordia. Seed Science Research 7, 169–178.CrossRefGoogle Scholar
Mudgett, M.B., Lowensen, J.D. and Clarke, S. (1997) Protein repair L-isoaspartyl methyltransferase in plants. Phylogenetic distribution and the accumulation of substrate proteins in aged barley seeds. Plant Physiology 115, 1481–1489.CrossRefGoogle ScholarPubMed
Mycock, D.J. and Berjak, P. (1995) The implications of seedassociated mycoflora during storage. pp. 747–766in Kigel, J..; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker Inc.Google Scholar
Mycock, D.J., Berjak, P. and Finch-Savage, W.E. (in preparation) Effects of desiccation on the sub-cellular matrix of the embryonic axes of Quercus robur in Black, M..; Bradford, K.J..; Vázquez-Ramos, J.M. (eds) Sixth International Workshop on Seeds. Wallingford, UK, CAB International.Google Scholar
Normah, M.N., Chin, H.F. and Hor, Y.L. (1986) Desiccation and cryopreservation of embryonic axes of Hevea brasiliensis Muell.-Arg. Pertanika 9, 299–303.Google Scholar
Ntuli, T.M., Berjak, P., Pammenter, N.W. and Smith, M.T. (1997) Effects of temperature on the desiccation responses of seeds of Zizania palustris. Seed Science Research 7, 145–160.CrossRefGoogle Scholar
Obendorf, R.L. (1997) Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance. Seed Science Research 7, 63–74.CrossRefGoogle Scholar
Oliver, M.J. and Bewley, J.D. (1997) Desiccation-tolerance of plant tissues: A mechanistic overview. Horticultural Reviews 18, 171–213.Google Scholar
Osborne, D.J. (1980) Senescence in seeds. pp. 13–37in Thimann, K.V. (Ed.) Senescence in plants. Boca Raton, FL, CRC Press.Google Scholar
Osborne, D.J. and Boubriak, I.I. (1994) DNA and desiccation tolerance. Seed Science Research 4, 175–185.CrossRefGoogle Scholar
Pammenter, N.W., Farrant, J.M. and Berjak, P. (1984) Recalcitrant seeds: short-term storage effects in Avicennia marina (Forsk.) Vierh. may be germination-associated. Annals of Botany 54, 843–846.CrossRefGoogle Scholar
Pammenter, N.W., Vertucci, C.W. and Berjak, P. (1991) Homeohydrous (recalcitrant) seeds: dehydration, the state of water and viability characteristics in Landolphia kirkii. Plant Physiology 96, 1093–1098.CrossRefGoogle ScholarPubMed
Pammenter, N.W., Vertucci, C.W. and Berjak, P. (1993) Responses of dehydration in relation to non-freezable water in desiccation-sensitive and -tolerant seeds. pp. 867–872in Côme, D..; Corbineau, F. (Eds) Fourth International Workshop on seeds: Basic and applied aspects of seed biology. Paris, ASFIS.Google Scholar
Pammenter, N.W., Motete, N. and Berjak, P. (1997) The response of hydrated recalcitrant seeds to long-term storage. pp. 673–687in Ellis, R.H..; Black, M..; Murdoch, A.J..; Hong, T.D. (Eds) Basic and applied aspects of seed biology. Kluwer Academic Publishers, Dordrecht, Netherlands.CrossRefGoogle Scholar
Pammenter, N.W., Berjak, P., Farrant, J.M., Smith, M.T. and Ross, G. (1994) Why do stored, hydrated recalcitrant seeds die? Seed Science Research 4, 187–191.CrossRefGoogle Scholar
Pammenter, N.W., Greggains, V., Kioko, J.I., Wesley-Smith, J., Berjak, P. and Finch-Savage, W.E. (1998) Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis. Seed Science Research 8, 463–471.CrossRefGoogle Scholar
Piatkowski, D., Schneider, K., Salamini, F. and Bartels, D. (1990) Characterization of five abscisic acid-responsive cDNA clones from the desiccation-tolerant plant Craterostigma plantagineum and their relationship to other water-stress genes. Plant Physiology 94, 1682–1688.CrossRefGoogle ScholarPubMed
Poulsen, K.N. and Eriksen, E.N. (1992) Physiological aspects of recalcitrance in embryonic axes of Quercus robur L. Seed Science Research 2, 215–221.CrossRefGoogle Scholar
Pritchard, H.W. (1991) Water potential and embryonic axis viability in recalcitrant seeds of Quercus rubra. Annals of Botany 67, 43–49.CrossRefGoogle Scholar
Pritchard, H.W. and Manger, K.R. (1998) A calorimetric perspective on desiccation stress during preservation procedures with recalcitrant seeds of Quercus robur L. CryoLetters 19(Supplement 1), 23–30.Google Scholar
Pritchard, H.W., Tompsett, P.B., Manger, K. and Smidt, W.J. (1995) The effect of moisture content on the low temperature response of Araucaria huntsteinii seed and embryos. Annals of Botany 76, 79–88.CrossRefGoogle Scholar
Pritchard, H.W., Tompsett, P.B. and Manger, K. (1996) Development of a thermal time model for the quantification of dormancy loss in Aesculus hippocastanum seeds. Seed Science Research 6, 127–135.CrossRefGoogle Scholar
Ried, J.L. and Walker-Simmons, M.K. (1993) Group 3 late embryogenesis abundant proteins in desiccationtolerant seedlings of wheat (Triticum aestivum L.). Plant Physiology 102, 125–131.CrossRefGoogle ScholarPubMed
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.Google Scholar
Roberts, E.H. and Ellis, R.H. (1989) Water and seed survival. Annals of Botany 63, 39–52.CrossRefGoogle Scholar
Rogerson, N.E. and Matthews, S. (1977) Respiratory and carbohydrate changes in developing pea (Pisum sativum) seeds in relation to their ability to withstand desiccation. Journal of Experimental Botany 28, 304–313.CrossRefGoogle Scholar
Russouw, P.S., Farrant, J.M., Brandt, W. and Lindsey, G.G. (1997) The most prevalent protein in a heat-treated extract of pea (Pisum sativum) is a LEA group I protein; its conformation is not affected by exposure to high temperature. Seed Science Research 7, 117–123.CrossRefGoogle Scholar
Sacandé, M., Groot, S.P.C., Hoekstra, F.A., de Castro, R.D. and Bino, R.J. (1997) Cell cycle events in developing neem (Azadirachta indica) seeds: are they related to intermediate storage behaviour? Seed Science Research 7, 161–168.CrossRefGoogle Scholar
Saenger, W., Hunter, W.N. and Kennard, O. (1986) DNA conformation is determined by economics in the hydration of phosphate groups. Nature 324, 385–388.CrossRefGoogle ScholarPubMed
Sargent, J.A., SenMandi, S. and Osborne, D.J. (1981) The loss of desiccation tolerance during germination: an ultrastructural and biochemical approach. Protoplasma 105, 225–239.CrossRefGoogle Scholar
Sen, S. and Osborne, D.J. (1974) Germination of rye embryos following hydration-dehydration treatments: enhancement of protein and RNA synthesis and earlier induction of DNA replication. Journal of Experimental Botany 25, 1010–1019.CrossRefGoogle Scholar
Senaratna, T. and McKersie, B.D. (1986) Loss of desiccation tolerance during seed germination: a free radical mechanism of injury. pp. 85–101in Leopold, A.C. (Ed.) Membranes, metabolism and dry organisms. Ithaca, London, Comstock.Google Scholar
Sherwin, H.W. and Farrant, J.M. (1996) Differences in rehydration of three desiccation-tolerant angiosperm species. Annals of Botany 78, 703–710.CrossRefGoogle Scholar
Sherwin, H.W. and Farrant, J.M. (1998) Protection mechanisms against excess light in the resurrection plants Craterostigma wilmsii and Xerophyta viscosa. Plant Growth Regulation 24, 203–210.CrossRefGoogle Scholar
Smirnoff, N. (1993) The role of active oxygen in the response of plants to water deficit and desiccation. Tansley Review No. 52 New Phytologist 125, 27–58.CrossRefGoogle Scholar
Smith, M.T. and Berjak, P. (1995) Deteriorative changes associated with the loss of viability of stored desiccationtolerant and -sensitive seeds. pp. 701–746in Kigel, J..; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker Inc.Google Scholar
Sowa, S., Roos, E.E. and Zee, F. (1991a) Anesthetic storage of recalcitrant seed: nitrous oxide prolongs longevity of lychee and longan. HortScience 26, 597–599.CrossRefGoogle Scholar
Sowa, S., Vertucci, C.W., Crane, J., Pammenter, N.W. and Berjak, P. (1991b) FTIR analyses of desiccation in seeds of tea. Agronomy Abstracts, p. 170.Google Scholar
Spector, D.L. (1993) Macromolecular domains within the cell nucleus. Annual Review of Cell Biology 9, 265–315.CrossRefGoogle ScholarPubMed
Steadman, K.J., Pritchard, H.W. and Dey, P.M. (1996) Tissue-specific soluble sugars in seeds as indicators of storage category. Annals of Botany 77, 667–674.CrossRefGoogle Scholar
Still, D.W., Kovach, D.A. and Bradford, K.J. (1994) Development of desiccation tolerance in rice (Oryza sativa) and wild rice (Zizania palustris). Dehydrin expression, abscisic acid content, and sucrose accumulation. Plant Physiology 104, 431–438.CrossRefGoogle ScholarPubMed
Sugita, M., Yoshida, K. and Sasaki, K. (1979) Germinationinduced changes in chromosomal proteins of spring wheat and winter wheat embryos. Plant Physiology 64, 780–785.CrossRefGoogle ScholarPubMed
Sun, W.Q. and Leopold, A.C. (1993) The glassy state and accelerated aging of soybean seeds. Plant Physiology 89, 767–774.CrossRefGoogle Scholar
Sun, W.Q., Irving, T.C. and Leopold, A.C. (1994) The role of sugar, vitrification and membrane transition in seed desiccation-tolerance. Physiologia Plantarum 90, 621–628.CrossRefGoogle Scholar
Suszka, B. and Tylkowski, T. (1980) Storage of acorns of the English oak (Quercus robur L.) over 1–5 winters. Arboretum Kórnickie 25, 199–229.Google Scholar
Tommasi, F., Paciolla, C. and Arrigoni, O.. The ascorbate system in recalcitrant and orthodox seeds. Physiologia Plantarum in press.Google Scholar
Tompsett, P.B. (1983) The influence of gaseous environment on the storage life of Araucaria hunsteinii seed. Annals of Botany 52, 229–237.CrossRefGoogle Scholar
Tompsett, P.B. (1992) A review of the literature on storage of dipterocarp seeds. Seed Science and Technology 20, 251–267.Google Scholar
Tompsett, P.B. and Pritchard, H.W. (1993) Water status changes during development in relation to the germination and desiccation tolerance of Aesculus hippocastanum L. seeds. Annals of Botany 71, 107–116.CrossRefGoogle Scholar
Tompsett, P.B. and Pritchard, H.W. (1998) The effect of chilling and moisture status on the germination, desiccation tolerance and longevity of Aesculus hippocastanum L. seed. Annals of Botany 82, 249–261.CrossRefGoogle Scholar
Tuba, Z., Lichtenthaler, H.K., Csintalan, Z., Nagy, Z. and Szente, K. (1996) Loss of chlorophyllous, cessation of photosynthetic CO2 assimilation and respiration in the poikilochlorophyllous plant Xerophyta scabrida during desiccation. Physiologia Plantarum 96, 383–388.CrossRefGoogle Scholar
Tylkowski, T. (1977) Cold storage of Quercus robur L. acorns in an atmosphere of increased content of CO2 and a reduced O2 level. Arboretum Kórnickie 22, 275–283.Google Scholar
Vertucci, C.W. (1989) The effects of low water contents on physiological activities of seeds. Physiologia Plantarum 77, 172–176.CrossRefGoogle Scholar
Vertucci, C.W. (1990) Calorimetric studies on the state of water in seed tissues. Biophysical Journal 58, 1463–1471.CrossRefGoogle ScholarPubMed
Vertucci, C.W. (1993) Towards a unified hypothesis of seed aging. pp. 739–746in Côme, D..; Corbineau, F. (Eds) Fourth International Workshop on seeds: Basic and applied aspects of seed biology. Paris, ASFIS.Google Scholar
Vertucci, C.W. and Farrant, J.M. (1995) Acquisition and loss of desiccation tolerance. pp. 237–271in Kigel, J..; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker Inc.Google Scholar
Vertucci, C.W. and Leopold, A.C. (1984) Bound water in soybean seed and its relation to respiration and imbibitional damage. Plant Physiology 75, 114–117.CrossRefGoogle ScholarPubMed
Vertucci, C.W. and Leopold, A.C. (1986) Physiological activities associated with hydration level in seeds. pp. 35–49in Leopold, A.C. (Ed.) Membranes, metabolism and dry organisms. Ithaca, London, Comstock.Google Scholar
Vertucci, C.W. and Leopold, A.C. (1987) Water binding in legume seeds. Plant Physiology 85, 224–231.CrossRefGoogle ScholarPubMed
Vertucci, C.W. and Roos, E.E. (1990) Theoretical basis of protocols for seed storage. Plant Physiology 94, 1019–1023.CrossRefGoogle ScholarPubMed
Vertucci, C.W. and Roos, E.E. (1993) Theoretical basis of protocols for seed storage II. The influence of temperature on optimal moisture contents. Seed Science Research 3, 201–213.CrossRefGoogle Scholar
Vertucci, C.W., Crane, J., Porter, R.A. and Oelke, E.A. (1994) Physical properties of water in Zizania embryos in relation to maturity status, water content and temperature. Seed Science Research 4, 211–224.CrossRefGoogle Scholar
Vertucci, C.W., Crane, J., Porter, R.A. and Oelke, E.A. (1995) Survival of Zizania embryos in relation to water content, temperature and maturity status. Seed Science Research 5, 31–40.CrossRefGoogle Scholar
von Teichman, I. and van Wyk, A.E. (1994) Structural aspects and trends in the evolution of recalcitrant seeds in the dicotyledons. Seed Science Research 4, 225–239.CrossRefGoogle Scholar
Walters, C., Ried, J.L. and Walker-Simmons, M.K. (1997) Heat-soluble proteins extracted from wheat embryos have tightly bound sugars and unusual hydration properties. Seed Science Research 7, 125–134.CrossRefGoogle Scholar
Wesley-Smith, J., Vertucci, C.W., Berjak, P., Pammenter, N.W. and Crane, J. (1992) Cryopreservation of desiccation-sensitive axes of Camellia sinensis in relation to dehydration, freezing rate and the thermal properties of tissue water. Journal of Plant Physiology 140, 596–604.CrossRefGoogle Scholar
Wesley-Smith, J., Berjak, P., Pammenter, N.W. and Vertucci, C.W. (1995) Ultrastructural evidence for the effects of freezing in embryonic axes of Pisum sativum L. at various water contents. Annals of Botany 76, 59–64.CrossRefGoogle Scholar
Williams, R.J. and Leopold, A.C. (1989) The glassy state in corn embryos. Plant Physiology 89, 977–981.CrossRefGoogle Scholar
Wolfe, S.L. (1995) An Introduction to Cell and Molecular Biology. Belmont, California, Wadsworth.Google Scholar
Xia, Q.H., Chen, R.Z. and Fu, J.R. (1992) Moist storage of lychee (Litchi chinensis Sonn.) and longan (Euphoria longan Steud.) seeds. Seed Science and Technology 20, 269–279.Google Scholar