Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-22T04:42:37.041Z Has data issue: false hasContentIssue false
  • English
  • Français

Protein synthesis during osmopriming of leek (Allium porrum L.) seeds

Published online by Cambridge University Press:  19 September 2008

P. A. Davison  and
C. M. Bray
P. A. Davison
Affiliation:
Department of Biochemistry and Molecular Biology, University of Manchester, Oxford Road, Manchester M13 9PT, UK
C. M. Bray*
Affiliation:
Department of Biochemistry and Molecular Biology, University of Manchester, Oxford Road, Manchester M13 9PT, UK
*
* Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

An osmotic priming treatment of 14 days in a −1.0 MPa polyethylene glycol solution improves the germination performance of a highvigour seed lot of leek (Allium porrum L., cv. Verina). Using in vivo pulse-labelling, two-dimensional polyacrylamide gel electrophoresis and fluorography, five polypeptides were found to be synthesized in embryonic tissue at 14 days of priming that were not present at 4 days of germination without prior priming. These polypeptides were still synthesized at 6 h of germination following priming. This time point lies in the 6–12 h lag phase in protein synthesis previously observed in leek embryo tissue during germination of primed seeds where there is little increase in the rate of synthesis over that seen at the end of priming. None of the polypeptides was synthesized at 2 days of germination after priming, a period of seedling growth. The five polypeptides appear to be specifically associated with the priming period. Two additional polypeptides were found in leek embryos that were synthesized at higher levels at the end of priming than during germination alone. These continued to be synthesized, at lower levels in leek embryo tissue upon germination after priming. Several polypeptides were identified in leek endosperm tissue which were synthesized at higher levels during priming than during germination and also two polypeptides whose synthesis appeared to be specific to germination.

Keywords

Allium porrum L.germinationleeksosmoprimingprotein synthesisseeds
Type
Research Papers
Information
Seed Science Research , Volume 1 , Issue 1 , March 1991 , pp. 29 - 35
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

1

Current address: Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK

References

Bonner, W.M. and Laskey, R.A. (1974) A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46, 83–88.CrossRefGoogle ScholarPubMed
Bray, C.M., Davison, P.A., Ashraf, M. and Taylor, R.M. (1989) Biochemical changes during osmopriming of leek seeds. Annals of Botany 63, 185–193.Google Scholar
Brocklehurst, P.A. and Dearman, J. (1983) Interactions between seed priming treatments and nine seed-lots of carrot, celery and onion. II. Seedling emergence and plant growth. Annals of Applied Biology 102, 585–593.CrossRefGoogle Scholar
Brocklehurst, P.A., Dearman, J. and Drew, R.L.K. (1984) Effects of osmotic priming on seed germination and seedling growth in leek. Scientia Horticulturae 24, 201–210.CrossRefGoogle Scholar
Burgass, R.W. and Powell, A.A. (1984) Evidence for repair processes in the invigoration of seeds by hydration. Annals of Botany 53, 753–757.CrossRefGoogle Scholar
Coolbear, P. and Grierson, D. (1979) Studies on the changes in the major nucleic acid components of tomato seeds resulting from osmotic presowing treatment. Journal of Experimental Botany 30, 1153–1162.CrossRefGoogle Scholar
Cuming, A.C. and Lane, B.G. (1979) Protein synthesis in imbibing wheat embryos. European Journal of Biochemistry 99, 217–224.CrossRefGoogle ScholarPubMed
Czarnecka, E., Edelman, L., Schoffl, F. and Key, J.L. (1984) Induction of heat shock protein mRNA in maize mesocotyls by water stress, abscisic acid and wounding. Plant Molecular Biology 3, 45–50.CrossRefGoogle Scholar
Dell 'Aquila, A. and Bewley, J.D. (1989) Protein synthesis in the axes of PEG treated pea seed and during subsequent germination. Journal of Experimental Botany 40, 1001–1007.CrossRefGoogle Scholar
Dell 'Aquila, A. and Taranto, G. (1986) Cell division and DNA synthesis during osmopriming treatment and following germination in aged wheat embryos. Seed Science and Technology 14, 333–341.Google Scholar
Durrant, M.J., Payne, P.A. and McLaren, J.S. (1983) The use of water and some inorganic salt solutions to advance sugar beet seed. II. Experiments under controlled and field conditions. Annals of Applied Biology 103, 517–526.CrossRefGoogle Scholar
Fu, J.R. Lu, X.H., Chen, R.Z., Zhang, B.Z., Liui, Z.S., Li, Z.S. and Cai, D.Y. (1988) Osmoconditioning of peanut seeds with PEG to improve vigour and some biochemical activities. Seed Science and Technology 16, 197–212.Google Scholar
Heikkila, J.J., Papp, J.E.T., Schultz, G.A. and Bewley, J.D. (1984) Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs. Plant Physiology 76, 270–274.CrossRefGoogle Scholar
Heydecker, W. and Coolbear, P. (1977) Seed treatments for improved performance—survey and attempted prognosis. Seed Science and Technology 5, 353–425.Google Scholar
Khan, A.A., Tao, K.L., Knypl, J.S., Borkowaska, B. and Powell, L.E. (1978) Osmotic conditioning of seeds: Physiological and biochemical changes. Acta Horticulturae 83, 267–278.CrossRefGoogle Scholar
Laskey, R.A. and Mills, A.O. (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56, 335–341.CrossRefGoogle Scholar
Mazor, L., Perl, M. and Negbi, M. (1984) Changes in some ATP-dependent activities in seeds during treatment with PEG and during the redrying process. Journal of Experimental Botany 35, 1119–1127.CrossRefGoogle Scholar
Szafirowska, A., Khan, A.A. and Peck, N.H. (1981) Osmo-conditioning of carrot seed to improve seedling establishment and yield in cold soil. Agronomic Journal 73, 845–848.CrossRefGoogle Scholar
Thomas, T.H. (1981) Seed treatments and techniques to improve germination. Scientific Horticulture 32, 47–59.Google Scholar
Zanzoterra, P.L. and Bray, C.M. (1989) Biochemical and physiological changes during osmotic priming and germination of leek seeds. Plant Physiology(Life Science Advances) 8, 11–16.Google Scholar