Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-23T10:11:09.933Z Has data issue: false hasContentIssue false
  • English
  • Français

Influence of temperature on the Pfr level required for germination in lettuce cv. Grand Rapids

Published online by Cambridge University Press:  19 September 2008

David N. Kristie  and
Anthony Fielding
David N. Kristie*
Affiliation:
Department of Biology, Acadia University, Wolfville, Nova Scotia, B0P 1X0, Canada
Anthony Fielding
Affiliation:
Department of Biology, Acadia University, Wolfville, Nova Scotia, B0P 1X0, Canada
*
* Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

The effect of temperature on the Pfr level required to induce germination in lettuce cv. Grand Rapids was investigated. Various Pfr levels were established in seeds, by irradiation with different fluences of red light, or by saturating irradiations with narrow bandwidth light. Both approaches indicated that increasing Pfr levels caused a progressive increase in the upper temperature limit for germination. The Pfr level required to induce 50% germination after a single light pulse increased from about 11% at 15° and 20°C to 86% at 30.5°C. Repeated red light irradiations raised the GT50 (temperature giving 50% germination) by about 2°C, suggesting that Pfr reversion limited germination at high temperatures. However, a large increase in Pfr level required to induce 50% germination was still apparent at high temperatures, even when relatively constant levels of Pfr were maintained by repeated irradiations with narrow bandwidth light. These results indicate that following a single light pulse, germination at high temperatures is limited solely by the availability of Pfr. The precise position of the upper temperature limit for germination is influenced by reversion; however, the underlying cause of thermoinhibition relates to an increasing requirement for Pfr at elevated temperatures.

Keywords

germinationlettucephytochrometemperaturethermoinhibition
Type
Research papers
Information
Seed Science Research , Volume 4 , Issue 1 , March 1994 , pp. 19 - 25
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

1

Present address Department of Botany, University of British Columbia, Vancouver, B.C., V6L 1N9, Canada.

References

Bewley, J.D. and Black, M. (1982) Physiology and biochemistry of seeds in relation to germination. Volume 2. Berlin, Heidelberg, New York, Springer-Verlag.CrossRefGoogle Scholar
Borthwick, H.A., Hendricks, S.B., Toole, E.H. and Toole, V.K. (1954) Action of light on lettuce seed germination. Botanical Gazette 115, 205–225.CrossRefGoogle Scholar
Cone, J.W. and Kendrick, R.E. (1985) Fluence-response curves and action spectra for promotion and inhibition of seed germination in wildtype and long-hypocotyl mutants of Arabidopsis thaliana L. Planta 163, 43–54.CrossRefGoogle ScholarPubMed
Cone, J. and Kendrick, R.E. (1986) Photocontrol of seed germination. pp 443–465 in Kendrick, R.E., and Kronenburg, G.H.M. (Eds) Photomorphogenesis in Plants. Dordrecht, Boston, Lancaster, Martinus Nijhoff Publishers.CrossRefGoogle Scholar
Duke, S.O. (1978) Significance of fluence response data in phytochrome-initiated seed germination. Photochemistry and Photobiology 28, 383–388.CrossRefGoogle Scholar
Fielding, A., Kristie, D.N. and Dearman, P. (1992) The temperature dependence of Pfr action governs the upper temperature limit for germination in lettuce. Photochemistry and Photobiolology 58, 623–627.CrossRefGoogle Scholar
Frankland, B. and Letendre, R.J. (1978) Phytochrome and effects of shading on growth of woodland plants. Photochemistry and Photobiology 27, 223–230.CrossRefGoogle Scholar
Frankland, B. and Taylorson R. (1983) Light control of seed germination. pp 428–456 in Shropshire, W. and Mohr, H. (Eds) Encyclopedia of Plant Physiology Vol. 16A. Photomorphogenesis. Berlin, Springer-Verlag.Google Scholar
Georghiou, K. and Kendrick, R.E. (1991) The germination characteristics of phytochrome-deficient aurea mutant tomato seeds. Physiologia Plantarum 82, 127–133.CrossRefGoogle Scholar
Hegarty, T.W. and Ross, H.A. (1979) Effects of light and growth regulators on germination and radicle growth of lettuce seeds held under high-temperature stress and water stress. New Phytologist 82, 49–57.CrossRefGoogle Scholar
Hendricks, S.B. and Taylorson, R.B. (1976) Variation in germination and amino acid leakage of seeds with temperature related to membrane phase change. Plant Physiology 58, 7–11.CrossRefGoogle ScholarPubMed
Hendricks, S.B. and Taylorson R.B. (1978) Dependence of phytochrome action in seeds on membrane organization. Plant Physiology 61, 17–19.CrossRefGoogle ScholarPubMed
Hendricks, S.B. and Taylorson, R.B. (1979) Dependence of thermal responses of seeds on membrane transitions. Proceedings National Academy of Sciences, USA 76, 778–781.CrossRefGoogle ScholarPubMed
Hillhorst, H.W.M. (1990) Dose-response analysis of factors involved in germination and secondary dormancy of seeds of Sisymbrium officinale. Plant Physiology 94, 1090–1095.CrossRefGoogle Scholar
Ikuma, H. and Thimann, K.V. (1964) Analysis of germination processes of lettuce seed by means of temperature and anaerobiosisis. Plant Physiology 39, 756–767.CrossRefGoogle Scholar
Karssen, C.M. (1970) The light promoted germination of the seeds of Chenopodium album L. VI. Pfr reguirement during different stages of the germination process. Acta Botanica Neerlandica 19, 297–312.CrossRefGoogle Scholar
Kristie, D.N., Bassi, P.K. and Spencer, M.S. (1981) Factors affecting the induction of secondary dormancy in lettuce. Plant Physiology 67, 1224–1229.CrossRefGoogle ScholarPubMed
Orlandi, M. and Malcoste, R. (1972) An in vivo study of phytochrome in seeds of Pinus nigra by differential spectrophotomery. Planta 105, 310–316.CrossRefGoogle Scholar
Pons, T.L. (1986) Responses of Plantago major seeds to the red/far-red ratio as influenced by other environmental factors. Physiologia Plantarum 68, 252–258.CrossRefGoogle Scholar
Pons, T.L. (1992) Seed responses to light. pp 259–284 in Fenner, M. (Ed.) Seeds. The ecology of regeneration in plant communities. Wallingford, CAB International.Google Scholar
Reynolds, T. (1973) A temperature-dependent source of variability in estimates of germination behaviour of lettuce fruits. Planta 113, 327–332.CrossRefGoogle ScholarPubMed
Reynolds, T. and Thompson, P.A. (1971) Characterisation of the high temperature inhibition of germination of lettuce (Lactuca sativa). Physiologia Plantarum 24, 544–547.CrossRefGoogle Scholar
Reynolds, T. and Thompson, P.A. (1973) Effects of kinetin, gibberellins and (±)abscisic acid on the germination of lettuce (Lactuca sativa). Physiologia Plantarum 28, 516–522.CrossRefGoogle Scholar
Saini, H.S., Consolacion, E.D., Bassi, P.K. and Spencer, M.S. (1989) Control processes in the induction and relief of themoinhibition of lettuce seed germination. Actions of phytochrome and endogenous ethylene. Plant Physiology 90, 311–315.CrossRefGoogle ScholarPubMed
Schiebe, J. and Lang, A. (1965) Lettuce seed germination: Evidence for a reversible light-induced increase in growth potential and for phytochrome mediation of the low temperature effect. Plant Physiology 40, 485–492.CrossRefGoogle Scholar
Taylorson, R.B. and Hendricks, S.B. (1969) Action of phytochrome during prechilling of Amaranthus retroflexus L. seeds. Plant Physiology 44, 821–825.CrossRefGoogle ScholarPubMed
Toole, V.K. (1973) Effects of light, temperature and their interactions on the germination of seeds. Seed Science and Technology 1, 339–396.Google Scholar
VanDerWoude, W.J. (1985) A dimeric mechanism for the action of phytochrome: Evidence from photo-thermal interactions in lettuce seed germination. Photochemistry and Photobiology 42, 655–661.CrossRefGoogle Scholar
Vidaver, W. and Hsiao, A.I. (1975) Secondary dormancy in light-sensitive lettuce seeds incubated anaerobically or at elevated temperature. Canadian Journal of Botany 22, 2557–2560.CrossRefGoogle Scholar