Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-23T18:17:03.193Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of controlled deterioration and osmopriming on protein synthesis of cauliflower seeds during early germination

Published online by Cambridge University Press:  19 September 2008

Yuzo Fujikura  and
Cees M. Karssen
Yuzo Fujikura*
Affiliation:
Department of Plant Physiology, Agricultural University, Arboretumlaan 4,6703 BD, Wageningen, Netherlands; International Board for Plant Genetic Resources, Via delle Sette Chiese 142, 00145, Rome, Italy
Cees M. Karssen
Affiliation:
Department of Plant Physiology, Agricultural University, Arboretumlaan 4,6703 BD, Wageningen, Netherlands;
*
*Department of Plant Physiology, Agricultural UniversityWageningen, Netherlands
Get access Rights & Permissions [Opens in a new window]

Abstract

Profiles of proteins synthesized in vivo in radicle tips were compared among unaged, artificially aged (controlled deterioration: 10% moisture content at 42°C for 3 weeks), osmoprimed (−1.5 MPa polyethylene glycol 6000 at 20°C for 1 week), and artificially aged and subsequently osmoprimed cauliflower (Brassica oleracea L.) seeds. Germination and initiation of incorporation of 35S-methionine into radicle tips were delayed by controlled deterioration. Osmopriming accelerated these processes and also alleviated the delays. The labelled proteins were analysed by two-dimensional gel electrophoresis and fluorography. Proteins were found whose expression correlated with the rate of germination, and was reduced by controlled deterioration but enhanced by osmopriming. Subsequent osmopriming reversed the effect of controlled deterioration and even caused an enhancement up to the level of unaged osmoprimed seeds. These proteins appeared to be related to processes preceding visible germination. In unaged seeds, formation of the proteins in the radicle tip began upon imbibition but decreased towards visible germination. On silver-stained gels, one of the proteins was shown to be mobilized by the time of radicle protrusion, while all were found in dry seeds. Proteins whose pronounced expression was observed only after both treatments were also found.

Keywords

Brassica oleracea L.controlled deteriorationgerminationosmoprimingprotein synthesisseed vigour
Type
Research Papers
Information
Seed Science Research , Volume 2 , Issue 1 , March 1992 , pp. 23 - 31
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Berjak, P. and Villiers, T.A. (1972) Ageing in plant embryos. II. Age-induced damage and its repair during early germination. New Phytologist 71, 135–144.CrossRefGoogle Scholar
Bray, C.M., Davison, P.A., Ashraf, M. and Taylor, R.M. (1989) Biochemical changes during osmopriming of leek seeds. Annals of Botany 63, 185–193.CrossRefGoogle Scholar
Brocklehurst, P.A. and Fraser, R.S.S. (1980) Ribosomal RNA integrity and rate of seed germination. Planta 148, 417–421.CrossRefGoogle ScholarPubMed
Burgass, R.W. and Powell, A.A. (1984) Evidence for repair processes in the invigoration of seeds by hydration. Annals of Botany 53, 753–757.Google Scholar
Cheah, K.S.E. and Osborne, D.J. (1978) DNA lesions occur with loss of viability in embryos of ageing rye seed. Nature 272, 593–599.CrossRefGoogle ScholarPubMed
Clarke, N.A. and James, P.E. (1991) The effects of priming and accelerated ageing upon the nucleic acid content of leek seeds and their embryos. Journal of Experimental Botany 42, 261–268.CrossRefGoogle Scholar
Coolbear, P., Slater, R.J. and Bryant, J.A. (1990) Changes in nucleic acid levels associated with improved germination performance of tomato seeds afterlow temperature presowing treatment. Annals of Botany 65, 187–195.CrossRefGoogle Scholar
Davison, P.A. and Bray, C.M. (1991) Protein synthesis during osmopriming of leek (Allium porrum L.) seeds. Seed Science Research 1, 29–35.CrossRefGoogle Scholar
Dell'Aquila, A. and Bewley, J.D. (1989) Protein synthesis in the axes of polyethylene glycol-treated pea seed and during subsequent germination. Journal of Experimental Botany 40, 1001–1007.CrossRefGoogle Scholar
Dell'Aquila, A. and Taranto, G. (1986) Cell division and DNA-synthesis during osmopriming treatment and following germination in aged wheat embryos. Seed Science and Technology 14, 333–341.Google Scholar
Ellis, R.H. and Roberts, E.H. (1981) The quantification of ageing and survival in orthodox seeds. Seed Science and Technology 9, 379–409.Google Scholar
Gidrol, X., Noubhani, A. and Pradet, A. (1990) Biochemical changes induced by accelerated aging in sunflower seeds. II. RNA populations and protein synthesis. Physiologia Plantarum 80, 598–604.CrossRefGoogle Scholar
Heydecker, W., Higgins, J. and Gulliver, R.L. (1973) Accelerated germination by osmotic seed treatment. Nature 246, 42–44.CrossRefGoogle Scholar
International Seed Testing Association (1985) International rules for seed testing. Seed Science and Technology 13, 338–341.Google Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277, 680–685.CrossRefGoogle Scholar
Liou, T. (1987) Studies on germination and vigour of cabbage seeds. Thesis, Agricultural University Wageningen Netherlands.Google Scholar
Morrissey, J.H. (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117, 307–310.Google Scholar
O'Farrell, P.Z., Goodman, H.M. and O'Farrell, P.H. (1977) High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12, 1133–1142.Google Scholar
Pandy, D.K. (1989) Priming induced alleviation of the effects of natural ageing derived selective leakage of constituents in French bean seeds. Seed Science and Technology 17, 391–397.Google Scholar
Senaratna, T., Gusse, J.F. and McKersie, B.D. (1988) Age-induced changes in cellular membranes of imbibed soybean seed axes. Physiologia Plantarum 73, 85–91.CrossRefGoogle Scholar
Skriver, K. and Mundy, J. (1990) Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2, 503–512.Google ScholarPubMed
Woodstock, L.W. and Tao, K.J. (1981) Prevention of imbibitional injury in low vigor soybean embryonic axes by osmotic control of water uptake. Physiologia Plantarum 51, 133–139.CrossRefGoogle Scholar