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The effect of soaking injury in bean seeds on aspects of the oxidative pentose phosphate pathway in embryonic axes

Published online by Cambridge University Press:  19 September 2008

Johan C. Pretorius  and
J. G. Chris Small
Johan C. Pretorius
Affiliation:
Department of Botany and Genetics, University of the Orange Free State, PO Box 339, Bloemfontein 9300, South Africa
J. G. Chris Small*
Affiliation:
Department of Botany and Genetics, University of the Orange Free State, PO Box 339, Bloemfontein 9300, South Africa
*
* Correspondence
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Abstract

Submerging Phaseolus vulgaris cv. Top Crop seeds in air-saturated water for 16 h markedly depresses subsequent germination. This is termed soaking injury. Soaking injury does not occur in seeds soaked in CO2-saturated water. Previous studies have shown that soaking injury can be alleviated by drying seeds or removing seed coats. Submergence therefore leads to a situation in bean seeds which is similar to secondary dormancy.

As with dormant seeds, C6/C1 ratios of embryonic axes of seeds soaked in air-saturated water remained high (0.8–1.0) during and after soaking. This was paralleled by low activities of glucose-6-phosphate dehydrogenase (EC.1.1.49) and 6-phosphogluconate dehydrogenase (EC 1.1.1.44). In axes of seeds soaked in CO2-saturated water and in unsoaked seeds C6/C1 ratios declined steadily during soaking/imbibition and reached values of around 0.3 after germination. Slight increases ofglucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities occurred in the pre-germination phase. This was followed by a massive increase after radicle emergence. Synthesis of the plastid isoenzymes was a post-germinative event.

It appears that soaking injury depresses protein synthesis. Lack of oxidative pentose phosphate pathway activity appears to be a causative factor in soaking injury.

Keywords

Phaseolus vulgarisoxidative pentose phosphate pathwaysoaking injury
Type
Research Papers
Information
Seed Science Research , Volume 2 , Issue 1 , March 1992 , pp. 33 - 39
Copyright
Copyright © Cambridge University Press 1992

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Footnotes

1

Present address Faculty of Applied Sciences, Technikon of the Orange Free State, Private Bag X20539, Bloemfontein, South Africa

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