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The distribution of ATP within tomato (Lycopersicon esculentum Mill.) embryos correlates with germination whereas total ATP concentration does not

Published online by Cambridge University Press:  22 February 2007

Patrick Spoelstra ,
Ronny V.L. Joosen ,
Linus H.W. Van der Plas  and
Henk W.M. Hilhorst
Patrick Spoelstra
Affiliation:
Wageningen University, Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
Ronny V.L. Joosen
Affiliation:
Wageningen University, Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
Linus H.W. Van der Plas
Affiliation:
Wageningen University, Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
Henk W.M. Hilhorst*
Affiliation:
Wageningen University, Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
*
*Correspondence Fax: +31 317484740 Email: [email protected]
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Abstract

The distribution of ATP in tomato seeds was visualized by monitoring the luminescence of frozen sections on top of a gel containing all the components of the luciferase reaction, but excluding ATP. ATP was imaged in germinating tomato seeds at intervals of 3, 6, 17, 24 and 48 h and in seeds with primary or secondary dormancy. ATP was present mainly in the embryo and concentrated in the radicle tip towards the completion of germination. In contrast to germinating seeds, ATP was distributed more evenly in dormant seeds. For germination, the ratio of ATP concentration in the radicle tip versus cotyledons was decisive, rather than the absolute concentration.

Keywords

Lycopersicon esculentumtomatoATPluciferasegerminationdormancy
Type
Research Article
Information
Seed Science Research , Volume 12 , Issue 4 , December 2002 , pp. 231 - 238
Copyright
Copyright © Cambridge University Press 2002

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