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Development and regulation of (1→3,1→4)-β-glucan endohydrolases in germinating wheat (Triticum aestivum)

Published online by Cambridge University Press:  19 September 2008

Doreen M. L. Lai ,
Amanda M. Slade  and
Geoffrey B. Fincher
Doreen M. L. Lai
Affiliation:
Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia
Amanda M. Slade
Affiliation:
Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia
Geoffrey B. Fincher*
Affiliation:
Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia
*
* Correspondence
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Abstract

The development of (1→3,1→4)-β-glucan 4-glucanohydrolase (EC 3.2.1.73) has been examined in germinating wheat (Triticum aestivum cv. Millewa) grain, and in isolated aleurone layers and scutella. Activity is first detected in extracts of intact grain 2–3 days after the initiation of germination and thereafter increases until 6 days. In isolated aleurone layers, (1→3,1→4)-β-glucanase activity is secreted for up to 4 days. Treatment of aleurone layers with gibberellic acid (GA3) results in a 2-fold enhancement of secreted enzyme for the first 2 days, but activity decreases after 2 days. (1→3,1→4)-β-Glucanase secretion from GA3-treated aleurone layers clearly precedes the secretion of amylase and (1→4)-β-xylanase. Small but significant levels of cellulase are secreted from aleurone layers after GA3 treatment. Isolated scutella also secrete (1→3,1→4)-β-glucanase but GA3 has little apparent effect on secretion patterns from this tissue. Proteins secreted from excised aleurone layers and scutella, together with those in homogenates of intact, germinated grain, have been examined by Western blot analysis, using monoclonal antibodies against barley (1→3,1→4)-β-glucanases as probes. In each case the wheat (1→3,1→4)-β-glucanase is recognised only by the monoclonal antibody that is specific for barley isoenzyme El.

Keywords

aleuronegerminationgibberellic acidmonoclonal antibodiesscutellumwheat
Type
Research Papers
Information
Seed Science Research , Volume 3 , Issue 1 , March 1993 , pp. 65 - 73
Copyright
Copyright © Cambridge University Press 1993

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Footnotes

1

Present address: School of Chemistry, University of Melbourne, Parkville, Victoria 3052, Australia

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