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Decline in a seed-specific abscisic acid-responsive glycine-rich protein (GRPF1) mRNA may reflect the release of seed dormancy in Fagus sylvatica during moist prechilling

Published online by Cambridge University Press:  22 February 2007

Lars Christian Mortensen ,
Dolores Rodríguez ,
Gregorio Nicolás ,
Erik Nymann Eriksen  and
Carlos Nicolás
Lars Christian Mortensen*
Affiliation:
Department of Agricultural Sciences, Section for Horticulture, The Royal Veterinary and Agricultural University, Hoejbakkegaard Allé 21, Taastrup, DK-2630, Denmark
Dolores Rodríguez
Affiliation:
Departamento de Fisiologia Vegetal, Facultad de Biologia, Universidad de Salamanca, Salamanca, E-37007, Spain
Gregorio Nicolás
Affiliation:
Departamento de Fisiologia Vegetal, Facultad de Biologia, Universidad de Salamanca, Salamanca, E-37007, Spain
Erik Nymann Eriksen
Affiliation:
Department of Agricultural Sciences, Section for Horticulture, The Royal Veterinary and Agricultural University, Hoejbakkegaard Allé 21, Taastrup, DK-2630, Denmark
Carlos Nicolás
Affiliation:
Departamento de Fisiologia Vegetal, Facultad de Biologia, Universidad de Salamanca, Salamanca, E-37007, Spain
*
*Correspondence: Fax: +45 35 28 34 78 Email: [email protected]
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Abstract

Dormancy and desiccation tolerance were studied in Fagus sylvatica seeds subsequent to 0–26 weeks of either moist prechilling (5°C) or warm (15°C) pretreatment at a restricted water content of 34% (fresh weight basis), i.e. c. 5% below full imbibition. After the pretreatments, seeds were either fully imbibed at 5 or 15°C, or dried for 7 d to around 13% moisture content and then fully imbibed at 5°C. Most of the dormancy was released after 5 weeks of prechilling, with nearly 70% of the seeds germinating at 15°C, and dormancy was fully released after 11 weeks of prechilling. Most of the seed desiccation tolerance, measured as the final germination percentage for dried seeds, was lost between 11 and 13 weeks of prechilling. A further decline in desiccation tolerance was recorded from 13 to 19 weeks, with a moderate recovery after 26 weeks. Germination was compared with the expression of a previously described abscisic-acid-responsive GRPF1 mRNA for a glycine-rich protein in F. sylvatica. There was a clear decline in accumulation of the transcript in seeds during prechilling, but not during the warm pretreatment. The amount of GRPF1 expression correlated significantly (P < 0.001) with dormancy status.

Keywords

Fagus sylvaticamoist prechillingdormancydesiccation toleranceGRPF1expression
Type
Research Article
Information
Seed Science Research , Volume 14 , Issue 1 , March 2004 , pp. 27 - 34
Copyright
Copyright © Cambridge University Press 2004

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