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Cryopreservation of zygotic embryos and kernels of oil palm (Elaeis guineensis Jacq.)

Published online by Cambridge University Press:  19 September 2008

F. Engelmann ,
N. Chabrillange ,
S. Dussert  and
Y. Duval
F. Engelmann*
Affiliation:
ORSTOM, 911 av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
N. Chabrillange
Affiliation:
ORSTOM, 911 av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
S. Dussert
Affiliation:
ORSTOM, 911 av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
Y. Duval
Affiliation:
ORSTOM, 911 av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
*
*Present address; IPGRI, via delle Sette Chiese 142, 00145 Rome, Italy
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Abstract

In the present study, the efficiency of two cryopreservation strategies for oil palm (Elaeis guineensis Jacq.) was compared. If extracted from rehydrated kernels, 65% of the embryos desiccated to around 0.3 g H2O/g DW developed into plantlets after cryopreservation. In contrast, only 25% of embryos (0.12 g H2O/g DW) extracted from cryopreserved dry kernels developed into plantlets. However, this value was increased to 63% if kernels were partially rehydrated before freezing until the water content of embryos reached 0.3 g H2O/g DW. This study emphasizes the importance of partial rehydration of oil palm embryos before cryopreservation.

Keywords

Cryopreservationdesiccationoil palmrehydrationkernelszygotic embryos
Type
Research Papers
Information
Seed Science Research , Volume 5 , Issue 2 , June 1995 , pp. 81 - 86
Copyright
Copyright © Cambridge University Press 1995

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