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Comparisons between the roles of the fruit tissues, osmoticum and abscisic acid in maintaining tomato seed development and storage protein synthesis

Published online by Cambridge University Press:  19 September 2008

Tannis Berry  and
J. Derek Bewley
Tannis Berry
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J. Derek Bewley*
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
*
* Correspondence
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Abstract

Five distinct groups of storage proteins are synthesized by embryos during development in the fruit at 35 days after pollination (35 DAP); none of them is synthesized in germinating embryos 48 h after isolation (48 HASI) of the seeds from the fruit. Endo-β-mannanase, a marker of germination and seedling growth, is produced in the isolated seeds, but not in the developing seed in situ 35 DAP. When seeds at this stage are removed from the fruit, but remain in intimate contact with the locular and sheath tissue, they continue to synthesize storage proteins for at least 48 HASI. Removal of the sheath from seeds isolated 35 DAP stops storage protein synthesis, and increases endo-β-mannanase activity. Both abscisic acid (ABA) and osmoticum at physiological concentrations maintain the synthesis of storage proteins in seeds isolated 35 DAP for at least 48 HASI, although osmoticum is more effective in preventing their eventual germination. Thus the effects of the sheath and the locular tissue are mimicked by both ABA and osmoticum in relation to the maintenance of storage protein synthesis in seeds 35 DAP and in the suppression of endo-β-mannanase activity for at least 48 HASI.

Keywords

tomato seedseed development and germinationosmotic potentialabscisic acidendo-β-mannanase
Type
Research Papers
Information
Seed Science Research , Volume 3 , Issue 1 , March 1993 , pp. 25 - 34
Copyright
Copyright © Cambridge University Press 1993

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Footnotes

1

Present address: Petawawa National Forestry Institute, Forestry Canada, Chalk River, Ontario, K0J 1J0, Canada

References

Ackerson, R.C. (1984) Regulation of soybean embryogenesis by abscisic acid. Journal of Experimental Botany 35, 403–413.CrossRefGoogle Scholar
Adams, C.A. and Rinne, R.W. (1980) Moisture content as a controlling factor in seed development. International Review of Cytology 68, 1–8.CrossRefGoogle Scholar
Bartels, D., Engelhardt, K., Roncarati, R., Schneider, K., Rotter, M. and Salamini, F. (1991) An ABA and GA modulated gene expression in the barley embryo encodes an aldolase reductase related protein. EMBO Journal 10, 1037–1043.CrossRefGoogle Scholar
Barratt, D.H.P., Whitford, P.N., Cook, S.K., Butcher, G. and Wang, T.L. (1989) An analysis of seed development in Pisum sativum. VIII. Does abscisic acid prevent precocious germination and control storage protein synthesis? Journal of Experimental Botany 40, 1009–1014.CrossRefGoogle Scholar
Barthe, P., Boulon, B., Gedraud, M. and LePage-Degivry, M. (1986) Intracellular pH and catabolism: two factors determining the level of abscisic acid in embryos of Phaseolus vulgaris during maturation. Physiologie Végétale 24, 453–461.Google Scholar
Berry, T. (1991) Role of the fruit tissue, abscisic acid and osmoticum in maintaining developmental events and inhibiting precocious germination in tomato seeds. PhD Thesis. University of Guelph, Guelph, Ontario, Canada.Google Scholar
Berry, T. and Bewley, J.D. (1991) Seeds of tomato (Lycopersicon esculentum Mill.) which develop in a fully hydrated environment in the fruit switch from a developmental to a germinative mode without a requirement for desiccation. Planta 186, 27–34.CrossRefGoogle Scholar
Berry, T. and Bewley, J.D. (1992) A role for the surrounding fruit tissues in preventing germination of tomato (Lycopersicon esculentum) seeds. A consideration of the osmotic environment and abscisic acid. Plant Physiology 100, 951–957.CrossRefGoogle Scholar
Bray, E.A. and Beachy, R.N. (1985) Regulation by abscisic acid of β-conglycinin expression in cultured developing soybean cotyledons. Plant Physiology 79, 746–750.CrossRefGoogle ScholarPubMed
Crouch, M.L. and Sussex, I.M. (1981) Development and storage-protein synthesis in Brassica napus L. embryos in vivo and in vitro. Planta 153, 64–74.Google ScholarPubMed
Egli, D.B. (1990) Seed water relations and the regulation of the duration of seed growth in soybean. Journal of Experimental Botany 41, 243–248.CrossRefGoogle Scholar
Eisenberg, A.J. and Mascarenhas, J.P. (1985) Abscisic acid and the regulation of synthesis of specific seed proteins and their messenger RNAs during culture of soybean embryos. Planta 166, 505–514.CrossRefGoogle ScholarPubMed
Finkelstein, R. and Crouch, M.L. (1985) Control of embryo maturation in rapeseed, pp 157–165 in Freeling, M. (Ed.) Plant Genetics.New York, Alan R. Liss Inc.Google Scholar
Finkelstein, R. and Crouch, M.L. (1986) Rapeseed embryo development in culture on high osmoticum is similar to that in seeds. Planta Physiology 81, 907–912.CrossRefGoogle ScholarPubMed
Gifford, D.J. and Bewley, J.D. (1984) Synthesis of the crystalloid protein complex in vivo in the endosperm of developing castor bean seeds. Plant Physiology 74, 1006–1011.CrossRefGoogle ScholarPubMed
Groot, S.P.C. (1987) Hormonal regulation of seed development and germination in tomato. Studies on abscisic acidand gibberellin-deficient mutants. Ph.D. Thesis, Agricultural University, Wageningen, The Netherlands.Google Scholar
Hsu, F.C. (1979) Abscisic acid accumulation in developing seeds of Phaseolus vulgaris L. Plant Physiology 63, 552–556.CrossRefGoogle ScholarPubMed
Krochko, J.E. and Bewley, J.D. (1988) Use of electrophoretic techniques in determining the composition of seed storage proteins in alfalfa. Electrophoresis 9, 751–763.CrossRefGoogle ScholarPubMed
Le Page-Degivry, M.T., Barthe, P., Prevost, I. and Boulon, P. (1989) Regulation of abscisic acid translocation during embryo maturation of Phaseolus vulgaris. Physiologia Plantarum 77, 81–86.CrossRefGoogle Scholar
Long, S.R., Dale, R.M.K. and Sussex, I.M. (1981) Maturation and germination of Phaseolus vulgaris embryonic axes in culture. Planta 153, 405–415.CrossRefGoogle ScholarPubMed
Malek, L. and Bewley, J.D. (1991) Endo-β-mannanase activity and reserve mobilization in excised endosperms of fenugreek is affected by volume of incubation and abscisic acid. Seed Science Research 1, 45–49.CrossRefGoogle Scholar
Michel, B.E. and Kaufmann, M.R.E. (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiology 51, 914–916.CrossRefGoogle ScholarPubMed
O'Farrell, P.H. (1975) High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–4021.CrossRefGoogle ScholarPubMed
Pressey, R. (1989) Endo-β-mannanase in tomato fruit. Phytochemistry 28, 3277–3280.CrossRefGoogle Scholar
Shriver, K. and Mundy, J. (1990) Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2, 503–512.Google Scholar
Smith, O. (1935) Pollination and life-history studies of tomato (Lycopersicon esculentum Mill.). Cornell University Agricultural Station Memo 184, 3–16.Google Scholar
Towbin, J., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Science, USA 76, 4350–4354.CrossRefGoogle ScholarPubMed
Welbaum, G.E., Tissaoui, T. and Bradford, K.J. (1990) Water relations of seed development and germination in muskmelon (Cucumis melo L). III. Sensitivity of germination to water potential and abscisic acid during development. Plant Physiology 92, 1029–1037.CrossRefGoogle ScholarPubMed
Yeung, E.C. and Brown, D.C.W. (1982) The osmotic environment of developing embryos of Phaseolus vulgaris. Zeitschrift fur Pflanzenphysiologie 106, 149–156.CrossRefGoogle Scholar
Xu, N., Coulter, K.M. and Bewley, J.D. (1990) Abscisic acid and osmoticum prevent germination of developing alfalfa (Medicago sativa) embryos, but only osmoticum maintains the synthesis of developmental proteins. Planta 182, 382–390.CrossRefGoogle ScholarPubMed