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Changes in the synthesis of some proteins during breaking of secondarily induced thermodormancy by respiration inhibitors (cyanide, azide) and BAP in embryos of Agrostemma githago L. seeds

Published online by Cambridge University Press:  19 September 2008

D. Bernhardt  and
U. Gerth
D. Bernhardt*
Affiliation:
Botanisches Institut der Ernst-Moritz-Arndt-Universität, Grimmer-Str. 88, 17487 Greifswald, Germany
U. Gerth
Affiliation:
Botanisches Institut der Ernst-Moritz-Arndt-Universität, Grimmer-Str. 88, 17487 Greifswald, Germany
*
*Correspondence
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Abstract

Secondary dormancy (thermodormancy) in Agrostemma githago L. seeds, caused by imbibing the seeds for 4 d at 35°C in the dark, is broken under the influence of cyanide (10 mM), azide (1 mM or BAP (0.44 mM). Both respiration inhibitors (cyanide and azide) and BAP increase the synthesis of five specific soluble proteins in the embryo axes and cotyledons. The activity of β-cyanoalanine synthase (β-CAS, EC 4.4.1.9.), which is able to detoxify cyanide, decreased in response to cyanide and azide. The relevance of these findings to the breaking of thermodormancy is discussed.

Keywords

Agrostemma githago L.benzylamino-purine (BAP)thermodormancyβ-cyanoalanine synthase (β-CAS)germinationproteinrespiration inhibitors
Type
Research Papers
Information
Seed Science Research , Volume 4 , Issue 4 , December 1994 , pp. 415 - 420
Copyright
Copyright © Cambridge University Press 1994

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