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Changes in the synthesis of some proteins during breaking of secondarily induced thermodormancy by respiration inhibitors (cyanide, azide) and BAP in embryos of Agrostemma githago L. seeds

Published online by Cambridge University Press:  19 September 2008

D. Bernhardt  and
U. Gerth
D. Bernhardt*
Affiliation:
Botanisches Institut der Ernst-Moritz-Arndt-Universität, Grimmer-Str. 88, 17487 Greifswald, Germany
U. Gerth
Affiliation:
Botanisches Institut der Ernst-Moritz-Arndt-Universität, Grimmer-Str. 88, 17487 Greifswald, Germany
*
*Correspondence
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Abstract

Secondary dormancy (thermodormancy) in Agrostemma githago L. seeds, caused by imbibing the seeds for 4 d at 35°C in the dark, is broken under the influence of cyanide (10 mM), azide (1 mM or BAP (0.44 mM). Both respiration inhibitors (cyanide and azide) and BAP increase the synthesis of five specific soluble proteins in the embryo axes and cotyledons. The activity of β-cyanoalanine synthase (β-CAS, EC 4.4.1.9.), which is able to detoxify cyanide, decreased in response to cyanide and azide. The relevance of these findings to the breaking of thermodormancy is discussed.

Keywords

Agrostemma githago L.benzylamino-purine (BAP)thermodormancyβ-cyanoalanine synthase (β-CAS)germinationproteinrespiration inhibitors
Type
Research Papers
Information
Seed Science Research , Volume 4 , Issue 4 , December 1994 , pp. 415 - 420
Copyright
Copyright © Cambridge University Press 1994

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References

Bauw, G., van Damme, J., Puype, M., Vandekerckhove, J., Gesser, B., Ratz, G.P., Lauridsen, J.B. and Celis, J.E. (1989) Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels. Proceedings of the National Academy of Sciences, USA 86, 7701–7705.CrossRefGoogle ScholarPubMed
Bernhardt, D., Hecker, M. and Bernhardt, H. (1978) Eignung intakter Caryophyllaceen-Samen für die Bestimmung der DNA-, RNA- und Proteinsynthese durch Einbauversuche. Biochemie und Physiologie der Pflanzen 172, 263–270.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1982) Physiology and biochemistry of seeds in relation to germination. 2. Viability, dormancy and environmental control. Berlin, Heidelberg, New York, Springer-Verlag.CrossRefGoogle Scholar
Blumenthal, S.G., Hendrickson, H.R., Abrel, Y.P. and Conn, E.E. (1968) Cyanide metabolism in higher plants. III. The biosynthesis of β-cyanoalanine. Journal of Biological Chemistry 243, 5302–5307.CrossRefGoogle Scholar
Bogatek, R. and Lewak, S. (1988) Effects of cyanide and cold treatment on sugar catabolism in apple seeds during dormancy removal. Physiologia Plantarum 73, 406–411.CrossRefGoogle Scholar
Bogatek, R., Dziewanowska, K. and Lewak, S. (1991) Hydrogen cyanide and embryonal dormancy in apple seeds. Physiologia Plantarum 83, 417–421.CrossRefGoogle Scholar
Bonner, W.E. and Laskey, R.A. (1974) A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46, 83–88.CrossRefGoogle ScholarPubMed
Borriss, H. (1956) Über einige Ergebnisse und Probleme der Keimungsphysiologie. Wissenschaftliche Zeitschrift der Ernst-Moritz-Arndt-Universität Greifswald, Mathematisch-Naturwissenschaftliche Reihe 6, 251–265.Google Scholar
De Meillon, S., Small, J.G.C. and van de Venter, H.A. (1990) The respiratory metabolism of Strelitzia juncea Ait. seeds: The effect of dormancy release through oxygen incubation of the seeds on the activity of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. Journal of Experimental Botany 41, 709–714.CrossRefGoogle Scholar
Elthon, T.E. and McIntosh, L. (1987) Identification of the alternative terminal oxidase of higher plant mitochondria. Proceedings of the National Academy of Sciences, USA 84, 8399–8403.CrossRefGoogle ScholarPubMed
Esashi, Y., Komatsu, H., Ushizawa, R. and Sakai, Y. (1982) Breaking of secondary dormancy in cocklebur seeds by cyanide and azide in combination with ethylene and oxygen, and their effects on cytochrome and alternative respiratory pathways. Australian Journal of Plant Physiology 9, 97–111.Google Scholar
Esashi, Y., Isuzugawa, K., Matsuyama, S., Ashino, H. and Hasegawa, R. (1991) Endogenous evolution of HCN during pre-germination periods in many seeds species. Physiologia Plantarum 83, 27–33.CrossRefGoogle Scholar
Esen, A. (1978) A simple method for quantitative, semiquantitative, and qualitative assay of protein. Analytical Biochemistry 89, 264–273.CrossRefGoogle ScholarPubMed
Gahan, P.B., Dawson, A.L., Black, M. and Chapman, J.M. (1986) Localization of glucose-6-phosphate dehydrogenase activity in seeds and its possible involvement in dormancy breakage. Annals of Botany 57, 791–799.CrossRefGoogle Scholar
Khan, A.A. and Zeng, G.W. (1985) Dual action of respiratory inhibitors—Inhibition of germination and prevention of dormancy induction in lettuce seeds. Plant Physiology 77, 817–823.CrossRefGoogle ScholarPubMed
Lerbs, S., Lerbs, W., Wollgiehn, R. and Parthier, B. (1988) Cytokinin, light, and “developmental control” of protein synthesis in Cucurbita cotyledons. Botanica Acta 101, 283–365.Google Scholar
Manning, K. (1986) Ethylene production and β-cyanoalanine synthase activity in carnation flowers. Planta 168, 61–66.CrossRefGoogle ScholarPubMed
Mans, R.J. and Novelli, G.D. (1961) Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk method. Archives of Biochemistry and Biophysics 94, 48–53.CrossRefGoogle Scholar
Miller, J.M. and Conn, E.E. (1980) Metabolism of hydrogen cyanide by higher plants. Plant Physiology 65, 1199–1202.CrossRefGoogle ScholarPubMed
O'Farrell, P.H. (1975) High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–4021.CrossRefGoogle ScholarPubMed
Ohya, T. and Suzuki, H. (1990) Benzyladenine- and lightstimulated plastid protein synthesis in excised cucumber cotyledons. Plant Physiology and Biochemistry 28, 27–35.Google Scholar
Ordas, R.J., Fernandez, B. and Rodriguez, R. (1992) Benzyladenine-controlled protein synthesis and growth in apple cell suspensions. Physiologia Plantarum 84, 229–235.CrossRefGoogle Scholar
Roberts, E.H. (1969) Seed dormancy and oxidation processes. pp 161–192 in Woolhouse, H.W. (Ed.) Dormancy and survival. Symposium of the Society of Experimental Biology 23, Cambridge, Cambridge University Press.Google Scholar
Roberts, E.H. and Smith, R.D. (1977) Dormancy and the pentose phosphate pathway. pp 385–411 in Khan, A.A. (Ed.) The physiology and biochemistry of seed dormancy and germination. Amsterdam, New York, Oxford, Elsevier Biomedical Press.Google Scholar
Sakajo, S., Minagawa, N., Koniyama, T. and Yoshimoto, A. (1991) Molecular cloning of cDNA for antimycin A-inducible mRNA and its role in cyanide-resistant respiration in Hansenula anomala. Biochimica et Biophysica Acta 1090, 102–108.CrossRefGoogle ScholarPubMed
Satoh, S. and Esashi, Y. (1980) No contribution of the pentose phosphate pathway in dormancy-breaking of cocklebur seeds. Physiologia Plantarum 48, 243–246.CrossRefGoogle Scholar
Siegel, L.M. (1965) A direct microdetermination for sulfide. Analytical Biochemistry 11, 126–132.CrossRefGoogle ScholarPubMed
Simmonds, J.A. and Simpson, G.M. (1971) Increased participation of pentose phosphate pathway in response to after-ripening and gibberellic acid treatment in caryopses of Avena fatua. Canadian Journal of Botany 49, 1833–1840.CrossRefGoogle Scholar
Vazquez-Ramos, J.M. and Jimenez, J.R. (1990) Stimulation of DNA synthesis and DNA polymerase activity by benzyladenine during early germination of maize axes. Canadian Journal of Botany 68, 2590–2594.CrossRefGoogle Scholar
Yoshimoto, A., Sakajo, S., Minagawa, N. and Komiyama, T. (1989) Possible role of a 36 kDa protein induced by respiratory inhibitors in cyanide-resistant respiration in Hansenula anomala. Journal of Biochemistry 105, 864–866.CrossRefGoogle ScholarPubMed