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Can chlorophyll fluorescence be used to determine the optimal time to harvest rice seeds for long-term genebank storage?

Published online by Cambridge University Press:  08 April 2015

Fiona R. Hay ,
Stephen Timple  and
Bert van Duijn
Fiona R. Hay*
Affiliation:
T.T. Chang Genetic Resources Center, International Rice Research Institute, Los Baños, Philippines
Stephen Timple
Affiliation:
T.T. Chang Genetic Resources Center, International Rice Research Institute, Los Baños, Philippines
Bert van Duijn
Affiliation:
Plant Biodynamics Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands Fytagoras, Sylviusweg 72 2333 BELeiden, The Netherlands
*
*Correspondence E-mail: [email protected]
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Abstract

Chlorophyll fluorescence (CF) analysis was explored as a potential tool to identify the optimal time to harvest rice seed germplasm for maximum storage longevity. Seeds of 20 diverse genebank accessions were harvested at 24, 31, 38 and 45 d after peak flowering (DAF) and half of each seed lot was sorted by hand, following normal practice at the T.T. Chang Genetic Resources Center. CF analysis was carried out on both non-sorted and sorted seeds, while storage experiments were carried out on sorted seeds. Seed longevity (the time for viability to fall to 50%, p50) was significantly correlated with the skewness, kurtosis, mode and mean of the CF histograms when the data for every accession at all the harvest times were included in the correlation analysis. However, these correlation coefficients were ≤ 0.481. The correlation coefficient between p50 and DAF was similarly low (0.461). For individual accessions, there was wide variation in the correlation coefficients. While for some accessions, there appeared to be a strong relationship between p50 and mean CF that could be used to guide when to harvest seeds in the field, for other accessions, a unique mean CF to inform when to harvest seeds or for use in seed sorting could not be identified; this was also true for DAF over the harvesting schedule used in this study. Given the number and diversity of accessions managed by a genebank, it seems unlikely that CF analysis would be an appropriate tool to help manage the regeneration or processing of seeds intended for storage in the genebank.

Keywords

chlorophyll fluorescencerice (Oryza sativa L.)seed longevityseed maturity
Type
Research Papers
Information
Seed Science Research , Volume 25 , Issue 3 , September 2015 , pp. 321 - 334
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Copyright
Copyright © Cambridge University Press 2015 

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References

Cicero, S.M., van der Schoor, R. and Jalink, H. (2009) Use of chlorophyll fluorescence sorting to improve soybean seed quality. Revista Brasileira de Sementes 31, 145–151.CrossRefGoogle Scholar
Ellis, R.H. and Roberts, E.H. (1980) Improved equations for the prediction of seed longevity. Annals of Botany 45, 13–30.CrossRefGoogle Scholar
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1987) The development of desiccation tolerance and maximum seed quality during seed maturation in six grain legumes. Annals of Botany 59, 23–29.CrossRefGoogle Scholar
Hay, F.R. and Probert, R.J. (1995) The effect of different drying conditions and maturity on desiccation tolerance and seed longevity in Digitalis purpurea L. Annals of Botany 76, 639–647.CrossRefGoogle Scholar
Hay, F.R. and Probert, R.J. (2011) Collecting and handling seeds in the field. Chapter 20 in Guarino, L.; Ramanatha Rao, V.; Goldberg, E. (Eds) Collecting plant genetic diversity: Technical guidelines – 2011 update. Rome, Bioversity International. Available at http://cropgenebank.sgrp.cgiar.org/index.php/procedures-mainmenu-242/collecting (accessed accessed 6 March 2015).Google Scholar
Hay, F.R. and Probert, R.J. (2013) Advances in seed conservation of wild plant species: a review of recent research. Conservation Physiology 1, 1–11.CrossRefGoogle ScholarPubMed
Hay, F.R. and Smith, R.D. (2003) Seed maturity: when to collect seeds from wild plants. pp. 97–133 in Smith, R.D.; Dickie, J.B.; Linington, S.H.; Pritchard, H.W.; Probert, R.J. (Eds) Seed conservation: Turning science into practice. Richmond, UK, The Royal Botanic Gardens Kew.Google Scholar
Hay, F., Adams, J., Manger, K. and Probert, R. (2008) The use of non-saturated lithium chloride solutions for experimental control of seed water content. Seed Science and Technology 36, 737–746.CrossRefGoogle Scholar
Jalink, H., Frandas, A., van der Schoor, R. and Bino, R.J. (1998a) Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality. Scientia Agricola 55, 88–93.CrossRefGoogle Scholar
Jalink, H., van der Schoor, R., Frandas, A., van Pijlen, J.G. and Bino, R.J. (1998b) Chlorophyll fluorescence of Brassica oleracea seeds as a non-destructive marker for seed maturity and seed performance. Seed Science Research 8, 437–443.CrossRefGoogle Scholar
Kameswara Rao, N. and Jackson, M.T. (1996a) Seed longevity of rice cultivars and strategies for their conservation in genebanks. Annals of Botany 77, 251–260.CrossRefGoogle Scholar
Kameswara Rao, N. and Jackson, M.T. (1996b) Effect of sowing date and harvest time on longevity of rice seeds. Seed Science Research 7, 13–20.Google Scholar
Kebreab, E. and Murdoch, A.J. (1999) A quantitative model for loss of primary dormancy and induction of secondary dormancy in imbibed seeds of Orobanche spp. Journal of Experimental Botany 50, 211–219.CrossRefGoogle Scholar
Kenanoglu, B.B., Demir, I. and Jalink, H. (2013) Chlorophyll fluorescence sorting method to improve quality of Capsicum pepper seed lots produced from different maturity fruits. HortScience 48, 965–968.CrossRefGoogle Scholar
McNally, K.L., Child, K.L., Bohnert, R., Davidson, R.M., Zhao, K., Ulat, V.J., Zeller, G., Clark, R.M., Hoen, D.R., Bureau, T.E., Stokowski, R., Ballinger, D.G., Frazer, K.A., Cox, D.R., Padhukasahasram, B., Bustamante, C.D., Weigel, D., Mackill, D.J., Bruskiewich, R.M., Rätsch, G., Buell, C.R., Leung, H. and Leach, J.E. (2009) Genomewide SNP variation reveals relationships among landraces and modern varieties of rice. Proceedings of the National Academy of Sciences, USA 106, 12273–12278.CrossRefGoogle ScholarPubMed
Nijënstein, J.H. (2014) Chlorophyll fluorescence as an indicator of seed quality. Seed Testing International 147, 7–9.Google Scholar
Ooms, D. and Destain, M.F. (2011) Evaluation of chicory seeds maturity by chlorophyll fluorescence imaging. Biosystems Engineering 110, 168–177.CrossRefGoogle Scholar
Probert, R., Adams, J., Coneybeer, J., Crawford, A. and Hay, F. (2007) Seed quality for conservation is critically affected by pre-storage factors. Australian Journal of Botany 55, 326–355.CrossRefGoogle Scholar
Sinniah, U.R., Ellis, R.H. and John, P. (1998) Irrigation and seed quality development in rapid-cycling brassica: seed germination and longevity. Annals of Botany 82, 309–314.CrossRefGoogle Scholar
Sokal, R.R. and Rohlf, F.J. (1995) Biometry (3rd edition). New York, W.H. Freeman and Company.Google Scholar
TeKrony, D.M., Egli, D.B. and Phillips, A.D. (1980) Effect of field weathering on the viability and vigor of soybean seed. Agronomy Journal 72, 749–753.CrossRefGoogle Scholar
Ward, K., Scarth, R., Daun, J.K. and McVetty, P.B.E. (1992) Effects of genotype and environment on seed chlorophyll degradation during ripening in four cultivars of oilseed rape (Brassica napus). Canadian Journal of Plant Science 72, 643–649.CrossRefGoogle Scholar
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