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Biopolymer volume change and water clustering function of primed Vigna radiata seeds

Published online by Cambridge University Press:  22 February 2007

Wendell Q. Sun ,
Yongheng Liang ,
Shangzhi Huang  and
Jiarui Fu
Wendell Q. Sun*
Affiliation:
Department of Biological Sciences, National University of Singapore, Singapore LifeCell Corporation, One Millennium Way, Branchburg, NJ, 08876, USA
Yongheng Liang
Affiliation:
Department of Biological Sciences, National University of Singapore, Singapore
Shangzhi Huang
Affiliation:
Department of Biology and Biotechnology, Zhongshan University, Guangzhou, China
Jiarui Fu
Affiliation:
Department of Biology and Biotechnology, Zhongshan University, Guangzhou, China
*
*Correspondence Fax: +1 908 947 1085 Email: [email protected]
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Abstract

Osmotic priming reduces the longevity of Vigna radiata (mung bean) seeds during subsequent dry storage. The present study has investigated the relationship between volumetric changes, modified water sorption properties and the decrease in seed longevity after priming. Volumetric analysis of control embryos revealed two major dehydration-related volumetric contractions in the biopolymer matrix, reflecting structural reorganization in biopolymer matrix during drying. These contractions occurred at hydration from 0.29 to 0.23 g g–1 dw and from 0.18 to 0.11 g g–1 dw, respectively. Volumetric contractions were reduced significantly after priming and also occurred at higher water contents (i.e. 0.48–0.32 g g–1 dw and 0.21–0.11 g g–1 dw). Consequently, at the same water content, primed seed embryos had higher specific biopolymer volume and lower specific density than control embryos. Water sorption study showed that priming did not change the monolayer hydration value, but altered the properties of multilayer water sorption in seed axes. The analysis of water clustering function suggested that priming enhanced the water–water association in seed axes, but not in cotyledon tissues. Solid-state 1H-NMR (nuclear magnetic resonance) investigation confirmed that priming did not cause a significant change of water dynamic properties in cotyledon tissues at water contents below 0.20 g g–1 dw. The relevance of volumetric modifications of the biopolymer matrix and changes in water clustering function to the reduced seed longevity after priming is discussed. It is proposed that priming-induced increases in surface reactivity may enhance deteriorative chemical reactions in re-dried seed tissues.

Keywords

anhydrobiosisglassy statenuclear magnetic resonanceseed ageingseed primingVigna radiatawater clustering function
Type
Research Article
Information
Seed Science Research , Volume 13 , Issue 4 , December 2003 , pp. 287 - 302
Copyright
Copyright © Cambridge University Press 2003

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References

Alvarado, A.D. and Bradford, K.J. (1988) Priming and storage of tomato (Lycopersicon lycopersicum) seeds. I. Effect of storage temperature on germination rate and viability. Seed Science and Technology 16, 601–612.Google Scholar
Argerich, C.A. and Bradford, K.J. (1989) The effects of priming and ageing on seed vigour in tomato. Journal of Experimental Botany 40, 599–607.CrossRefGoogle Scholar
Argerich, C.A., Bradford, K.J. and Tarquis, A.M. (1989) The effects of priming and aging on resistance to deterioration of tomato seeds. Journal of Experimental Botany 40, 593–598.CrossRefGoogle Scholar
Barlow, E.W.R. and Haigh, A.M. (1987) Effect of seed priming on the emergence, growth and yield of UC 82B tomatoes in the field. Acta Horticulturae 200, 153–164.Google Scholar
Bernal-Lugo, I. and Leopold, A.C. (1995) Seed stability during storage: raffinose content and seed glassy state. Seed Science Research 5, 75–80.CrossRefGoogle Scholar
Bradford, K.J. (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience 21, 1105–1112.CrossRefGoogle Scholar
Bruni, F. and Leopold, A.C. (1992) Pools of water in anhydrobiotic organisms: a thermally stimulated depolarization current study. Biophysical Journal 63, 663–672.Google Scholar
Buitink, J., Claessens, M.M.A.E., Hemminga, M.A. and Hoekstra, F.A. (1998a) Influence of water content and temperature on molecular mobility and intracellular glasses in seeds and pollen. Plant Physiology 118, 531–541.CrossRefGoogle ScholarPubMed
Buitink, J., Walters, C., Hoekstra, F.A. and Crane, J. (1998b) Storage behavior of Typha latifolia pollen at low water contents: interpretation on the basis of water activity and glass concepts. Physiologia Plantarum 103, 145–153.CrossRefGoogle Scholar
Buitink, J., Hemminga, M.A. and Hoekstra, F.A. (2000a) Is there a role for oligosaccharides in seed longevity? An assessment of intracellular glass stability. Plant Physiology 122, 1217–1224.CrossRefGoogle Scholar
Buitink, J., Leprince, O., Hemminga, M.A. and Hoekstra, F.A. (2000b) Molecular mobility in the cytoplasm: An approach to describe and predict lifespan of dry germplasm. Proceedings of the National Academy of Sciences, USA 97, 2385–2390.CrossRefGoogle ScholarPubMed
Clegg, J.S. (1986) The physical properties and metabolic status of Artemia cysts at low water contents: the water replacement hypothesis. pp. 117–153. in Leopold, A.C. (Ed.) Membranes, metabolism and dry organisms. Ithaca, NY, Cornell University Press.Google Scholar
Dillon, S.R. and Dougherty, R.C. (2002) Raman studies of the solution structure of univalent electrolytes in water. Journal of Physical Chemistry A 106, 7647–7650.CrossRefGoogle Scholar
Dominguez, E. and Heredia, A. (1999) Water hydration in cutinized cell walls: a physico-chemical analysis. Biochimica et Biophysica Acta 1426, 168–176.CrossRefGoogle ScholarPubMed
Gurusinghe, S. and Bradford, K.J. (2001) Galactosyl-sucrose oligosaccharides and potential longevity of primed seeds. Seed Science Research 11, 121–133.Google Scholar
Hardegree, S.P. (1994) Drying and storage effects on germination of primed grass seeds. Journal of Range Management 47, 196–199.CrossRefGoogle Scholar
Hill, H.J., Taylor, A.G. and Min, T.G. (1989) Density separation of imbibed and primed vegetable seeds. Journal of the American Society for Horticultural Science 114, 661–665.CrossRefGoogle Scholar
Hoekstra, F.A., Haigh, A.M., Tetteroo, F.A.A., Van Roekel, T. (1994) Changes in soluble sugars in relation to desiccation tolerance in cauliflower seeds. Seed Science Research 4, 143–147.CrossRefGoogle Scholar
Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: dependence on flatulence-producing oligosaccharides and cyclitols – review and survey. Seed Science Research 4, 385–405.CrossRefGoogle Scholar
Khan, A.A. (1992) Preplant physiological seed conditioning. Horticultural Reviews 13, 131–181.CrossRefGoogle Scholar
Leopold, A.C. (1983) Volumetric components of seed imbibition. Plant Physiology 73, 677–680.CrossRefGoogle ScholarPubMed
Leopold, A.C., Sun, W.Q., Bernal-Lugo, I. (1994) The glassy state in seeds: analysis and function. Seed Science Research 4, 267–274.CrossRefGoogle Scholar
Lin, T.P. and Huang, N.H. (1994) The relationship between carbohydrate composition of some tree seeds and their longevity. Journal of Experimental Botany 45, 1289–1294.CrossRefGoogle Scholar
Liu, Y., van der Burg, W.J., Aartse, J.W., van Zwol, R.A., Jalink, H. and Bino, R.J. (1993) X-ray studies on changes in embryo and endosperm morphology during priming and imbibition of tomato seeds. Seed Science Research 3, 171–178.CrossRefGoogle Scholar
Luque, P., Gavara, R. and Heredia, A. (1995) A study of the hydration process of isolated cuticular membranes. New Phytologist 129, 283–288.CrossRefGoogle ScholarPubMed
Mathur-de Vre, R. (1979) The NMR studies of water in biological systems. Progress in Biophysics and Molecular Biology 35, 103–134.Google Scholar
McDonald, M.B. (1999) Seed deterioration: physiology, repair and assessment. Seed Science and Technology 27, 177–237.Google Scholar
Murthy, U.M.N. and Sun, W.Q. (2000) Protein modification by the Amadori and Maillard reactions during seed storage: roles of sugar hydrolysis and lipid peroxidation. Journal of Experimental Botany 51, 1221–1228.CrossRefGoogle ScholarPubMed
Oluoch, M.O. and Welbaum, G.E. (1996) Viability and vigor of osmotically primed muskmelon seeds after nine years of storage. Journal of the American Society for Horticultural Science 121, 408–413.CrossRefGoogle Scholar
Parera, C.A. and Cantliffe, D.J. (1996) Presowing seed priming. Horticultural Reviews 16, 109–141.Google Scholar
Plonka, A. (1986) Time-dependence reactivity of species in condensed media, Lecture Notes in Chemistry, Vol. 40. Berlin, Springer-Verlag.Google Scholar
Plonka, A. (2001) Dispersive kinetics. Annual Reports of Progress in Chemistry Section C 97, 91–147.CrossRefGoogle Scholar
Probert, R.J., Bogh, S.V., Smith, A.J. and Wechsberg, G.E. (1991) The effect of priming on seed longevity in Ranunculus sceleratus L. Seed Science Research 1, 243–249.Google Scholar
Rahman, M.S. and Labuza, T.P. (1999) Water activity and food preservation. pp. 339–382. in Shafiur Rahman, M. (Ed.) Handbook of food preservation. New York, Marcel Dekker.Google Scholar
Ratkovic, S. (1987) Proton NMR of maize seed water: the relationship between spin-lattice relaxation time and water content. Seed Science and Technology 15, 147–154.Google Scholar
Rorschach, H.E. and Hazlewood, C.F. (1986) Protein dynamics and the NMR relaxation time T1 of water in biological systems. Journal of Magnetic Resonance 70, 79–88.Google Scholar
Seewaldt, V., Priestley, D.A., Leopold, A.C., Feigenson, G.W., Goodsaid-Zalduondo, F. (1981) Membrane organization in soybean seeds during hydration. Planta 152, 19–23.Google Scholar
Stannett, V.T., Ranade, G.R. and Koros, W.J. (1982) Characterization of water vapor transport in glassy polyacrylonitrile by combined permeation and sorption techniques. Journal of Membrane Science 10, 219–233.CrossRefGoogle Scholar
Steadman, K.J., Pritchard, H.W. and Dey, P.M. (1996) Tissue-specific soluble sugars in seeds as indicators of storage category. Annals of Botany 77, 667–674.CrossRefGoogle Scholar
Sun, W.Q. (1997) Glassy state and seed storage stability: the WLF kinetics of seed viability loss at T>Tg and the plasticization effect of water on storage stability. Annals of Botany 79, 291–297.CrossRefGoogle Scholar
Sun, W.Q. (1998) Function of the glassy state in seed storage stability. pp. 169–179. in Taylor, A.G.;Huang, X-L. (Eds) Progress in seed research: proceedings of the second international conference on seed science and technology.Geneva, New York,Communication Services New York State Agricultural Experiment Station.Google Scholar
Sun, W.Q. (2000) Dielectric relaxation of water and water-plasticized biomolecules in relation to cellular water organization, cytoplasmic viscosity, and desiccation tolerance in recalcitrant seed tissues. Plant Physiology 124, 1203–1215.CrossRefGoogle ScholarPubMed
Sun, W.Q. (2002) Methods for the study of water relations under desiccation stress. pp. 47–91. in Black, M.;Pritchard, H.W. (Eds) Desiccation and survival in plants: drying. without dying Wallingford, UK, CABI Publishing.CrossRefGoogle Scholar
Sun, W.Q. and Leopold, A.C. (1993) The glassy state and accelerated aging of soybeans. Physiologia Plantarum 89, 767–774.CrossRefGoogle Scholar
Sun, W.Q. and Leopold, A.C. (1994) Glassy state and seed storage stability: a viability equation analysis. Annals of Botany 74, 601–604.CrossRefGoogle Scholar
Sun, W.Q. and Leopold, A.C. (1995) The Maillard reaction and oxidative stress during aging of soybean seeds. Physiologia Plantarum 94, 94–105.CrossRefGoogle Scholar
Sun, W.Q. and Leopold, A.C. (1997) Cytoplasmic vitrification and survival of anhydrobiotic organisms. Comparative Biochemistry and Physiology 117, 327–333.CrossRefGoogle Scholar
Sun, W.Q., Koh, D.C.Y. and Ong, C.M. (1997) Correlation of modified water sorption properties with the decline of storage stability of osmotically-primed seeds of Vigna radiata (L.) Wilczek. Seed Science Research 7, 391–397.CrossRefGoogle Scholar
Tarquis, A.M. and Bradford, K.J. (1992) Prehydration and priming treatments that advance germination also increase the rate of deterioration of lettuce seeds. Journal of Experimental Botany 43, 307–317.Google Scholar
Vertucci, C.W. (1990) Calorimetric studies of the state of water in seed tissues. Biophysical Journal 58, 1463–1471.CrossRefGoogle ScholarPubMed
Vertucci, C.W. and Leopold, A.C. (1987a) Water binding in legume seeds. Plant Physiology 85, 224–231.Google Scholar
Vertucci, C.W. and Leopold, A.C. (1987b) The relationship between water binding and desiccation tolerance in tissues. Plant Physiology 85, 232–238.CrossRefGoogle ScholarPubMed
Vertucci, C.W. and Roos, E.E. (1990) Theoretical basis of protocols for seed storage. Plant Physiology 94, 1019–1023.CrossRefGoogle ScholarPubMed
Vertucci, C.W. and Roos, E.E. (1993) Theoretical basis of protocols for seed storage II. The influence of temperature on optimal moisture levels. Seed Science Research 3, 201–213.Google Scholar
Vertucci, C.W., Roos, E.E. and Crane, J. (1994) Theoretical basis of protocols for seed storage III: optimum moisture contents for pea seeds stored at different temperatures. Annals of Botany 74, 531–540.CrossRefGoogle Scholar
Williams, R.J. and Leopold, A.C. (1995) Changes in glass transition temperatures in germinating pea seeds. Seed Science Research 5, 117–120.CrossRefGoogle Scholar
Zimm, B.H. and Lundberg, J.L. (1956) Sorption of vapors by high polymers. Journal of Physical Chemistry 60, 425–428.CrossRefGoogle Scholar