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Biochemical characterization of atypical biotinylation domains in seed proteins

Published online by Cambridge University Press:  22 February 2007

Claudette Job ,
Stéphanie Laugel ,
Manuel Duval ,
Karine Gallardo  and
Dominique Job
Claudette Job
Affiliation:
Laboratoire Mixte CNRS/INRA/Aventis (UMR1932), Aventis CropScience, 14–20 rue Pierre Baizet, 69263, Lyon CEDEX 9, France
Stéphanie Laugel
Affiliation:
Laboratoire Mixte CNRS/INRA/Aventis (UMR1932), Aventis CropScience, 14–20 rue Pierre Baizet, 69263, Lyon CEDEX 9, France
Manuel Duval
Affiliation:
Department of Biology, Texas A & M University, College Station, TX 77843, USA
Karine Gallardo
Affiliation:
Laboratoire Mixte CNRS/INRA/Aventis (UMR1932), Aventis CropScience, 14–20 rue Pierre Baizet, 69263, Lyon CEDEX 9, France
Dominique Job*
Affiliation:
Laboratoire Mixte CNRS/INRA/Aventis (UMR1932), Aventis CropScience, 14–20 rue Pierre Baizet, 69263, Lyon CEDEX 9, France
*
*Correspondence Fax: (+33) 4 72 85 22 97 Email: [email protected]
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Abstract

Homologues of the pea SBP65, a late embryogenesis abundant (LEA) biotinylated protein that behaves as a putative sink for the free vitamin biotin during embryo development, were characterized biochemically in various plant species, including soybean, lentil, peanut, rape, cabbage, carrot and sugarbeet. Based on sequence homologies, the genome of Arabidopsis thaliana contains a gene putatively encoding a homologue of pea SBP65. These proteins exhibit two remarkable features. First, they only accumulate in seeds, particularly during late stages of embryo development. The results strongly suggest that these seed-specific biotinylated proteins belong to the class of plant proteins called seed maturation proteins, which are presumed to play major roles in embryo development. Secondly, covalent attachment of biotin occurs at a lysine residue within a conserved motif of (V/M)GKF, which shows no resemblance to the highly conserved AMKM tetrapeptide that houses the target lysine residue in the well-characterized biotin-dependent carboxylases and decarboxylases. These findings highlight novel structural features for protein biotinylation.

Keywords

seed maturationgerminationLEA proteinsbiotinylated proteinsbiotinArabidopsis thalianacabbage (Brassica oleracea)carrot (Daucus carota)lentil (Lens culinaris)pea (Pisum sativum)peanut (Arachis hypogaea)rape (Brassica napus)soybean(Glycine max (L.) Merrill)sugarbeet (Beta vulgaris)
Type
Research Article
Information
Seed Science Research , Volume 11 , Issue 2 , June 2001 , pp. 149 - 162
Copyright
Copyright © Cambridge University Press 2001

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