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Protection of Italian ryegrass (Lolium multiflorum L.) seedlings from salinity stress following seed priming with L-methionine and casein hydrolysate

Published online by Cambridge University Press:  07 December 2020

Keum-Ah Lee ,
Youngnam Kim ,
Hossein Alizadeh  and
David W.M. Leung Open the ORCID record for David W.M. Leung [Opens in a new window]
Keum-Ah Lee
Affiliation:
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch8040, New Zealand
Youngnam Kim
Affiliation:
Department of Ecology, Lincoln University, Lincoln7647, Christchurch, New Zealand
Hossein Alizadeh
Affiliation:
Bioprotection Research Centre, Lincoln University, PO BOX 85084, Canterbury, New Zealand
David W.M. Leung*
Affiliation:
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch8040, New Zealand
*
Correspondence: David W.M. Leung, E-mail: [email protected]
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Abstract

Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

Keywords

abiotic stressantioxidative defencegerminationperoxidase activitysalt stress
Type
Research Paper
Information
Seed Science Research , Volume 31 , Issue 1 , March 2021 , pp. 51 - 59
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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