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Phosphorylated 11S globulins in sunflower seeds

Published online by Cambridge University Press:  12 June 2013

Ivana Quiroga ,
Mariana Regente ,
Luciana Pagnussat ,
Ana Maldonado ,
Jesús Jorrín  and
Laura de la Canal
Ivana Quiroga
Affiliation:
Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata-CONICET, Mar del Plata, Argentina
Mariana Regente
Affiliation:
Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata-CONICET, Mar del Plata, Argentina
Luciana Pagnussat
Affiliation:
Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata-CONICET, Mar del Plata, Argentina
Ana Maldonado
Affiliation:
Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain
Jesús Jorrín
Affiliation:
Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain
Laura de la Canal*
Affiliation:
Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata-CONICET, Mar del Plata, Argentina
*
*Correspondence E-mail: [email protected]
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Abstract

Helianthinins are storage proteins present in Helianthus annuus seeds, belonging to the 11S globulin family. Here we describe that a fraction of the helianthinins is phosphorylated. This conclusion is supported by different criteria, including identification by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry of major protein bands revealed with a specific dye for phosphoproteins, anti-phosphoserine antibody and binding to a phosphoprotein affinity matrix. Moreover, we show that the phosphorylation status of helianthinins changes following germination.

Keywords

11S globulinsgerminationhelianthininHelianthus annuusphosphorylationstorage proteins
Type
Short Communication
Information
Seed Science Research , Volume 23 , Issue 3 , September 2013 , pp. 199 - 204
Copyright
Copyright © Cambridge University Press 2013 

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