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Non-deep complex morphophysiological dormancy in seeds of the Iberian Peninsula endemic geophyte Merendera montana (Colchicaceae)

Published online by Cambridge University Press:  05 August 2011

Elena Copete ,
José M. Herranz ,
Miguel A. Copete ,
Jerry M. Baskin  and
Carol C. Baskin
Elena Copete*
Affiliation:
ETSIA, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, Albacete 02071, Spain
José M. Herranz
Affiliation:
ETSIA, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, Albacete 02071, Spain
Miguel A. Copete
Affiliation:
ETSIA, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, Albacete 02071, Spain
Jerry M. Baskin
Affiliation:
Department of Biology, University of Kentucky, Lexington, KY 40506, USA
Carol C. Baskin
Affiliation:
Department of Biology, University of Kentucky, Lexington, KY 40506, USA Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA
*
*Correspondence Fax: +34 967 599238 Email: [email protected]
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Abstract

Heretofore, no detailed account was available on seed dormancy and germination of a member of the Colchicaceae (Liliales). Thus, the primary aim of this study was to do an in-depth investigation of the temperature requirements for dormancy break and germination in seeds of Merendera montana (Colchicaceae) at the embryo and whole-seed levels under near-natural temperatures in a non-heated frame shade-house and under controlled conditions in the laboratory. Mean embryo length in fresh seeds was c. 0.57 mm and embryos had to grow to at least 1.30 mm before radicle emergence. Embryos grew to full size and seeds completed germination (radicles emerged) when they were stratified at 28/14°C for 60 d followed by a cool temperature for 60 d and then incubated at a cool temperature for 30 d. The optimum cool stratification temperature for dormancy-break was 10°C. Thus, after the moist pretreatment at 28/14°C+10°C, radicle emergence was>93% at all incubation temperatures (5, 15/4 and 20/7°C). In its natural habitat, M. montana seeds are dispersed in June, the embryo elongates to full size in autumn and radicles emerge from early November to early February. Although the shoot does not emerge until March and April, it is not physiologically dormant. The shoot emerged from 80% of the radicle-emerged seeds in 13 d at 20/7°C without a previous cold pretreatment. Seeds of M. montana have non-deep complex morphophysiological dormancy, C1b1aB-C1a. This is the first study on seeds with complex MPD to show a delay in shoot emergence following root emergence despite the shoot being physiologically non-dormant.

Keywords

Colchicaceaeembryo growthgerminationgibberellic acidMerendera montanamorphophysiological dormancyseed germination
Type
Research Article
Information
Seed Science Research , Volume 21 , Issue 4 , December 2011 , pp. 267 - 281
Copyright
Copyright © Cambridge University Press 2011

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References

Ali, N., Probert, R., Hay, F., Davies, H. and Stuppy, W. (2007) Post-dispersal embryo growth and acquisition of desiccation tolerance in Anemone nemorosa L. seeds. Seed Science Research 17, 155–163.CrossRefGoogle Scholar
Antonidaki-Giatromanolaki, A., Dragassaki, M., Papadimitriou, M. and Vlahos, I. (2008) Effects of stratification, temperature and light on seed germination of Colchicum macrophyllum B. L. Burtt. Propagation of Ornamental Plants 8, 105–107.Google Scholar
Bain, G. and Lockwood, D. (1996) Some observations of the effect of smoke on the germination of south-eastern Australian native species. Combined Proceedings of the International Plant Propagators' Society 46, 146–148.Google Scholar
Barton, L.V. (1936) Germination and seedling production in Lilium sp[p]. Contributions from Boyce Thompson Institute 8, 297–309.Google Scholar
Barton, L.V. (1944) Some seeds showing special dormancy. Contributions from Boyce Thompson Institute 13, 259–271.Google Scholar
Baskin, C.C. and Baskin, J.M. (1988) Germination ecophysiology of herbaceous plant species in a temperature region. American Journal of Botany 75, 286–305.CrossRefGoogle Scholar
Baskin, C.C. and Baskin, J.M. (1998) Seeds: ecology, biogeography, and evolution of dormancy and germination. San Diego, USA, Academic Press.Google Scholar
Baskin, C.C. and Baskin, J.M. (2005) Seed dormancy in wild flowers. pp. 163–185 in McDonald, M.B.; Kwong, F.Y. (Eds) Flower seeds: biology and technology. Wallingford, UK, CAB International.CrossRefGoogle Scholar
Baskin, C.C., Meyer, S.E. and Baskin, J.M. (1995) Two types of morphophysiological dormancy in seeds of two genera (Osmorhiza and Erythronium) with an Arcto-Tertiary distribution pattern. American Journal of Botany 82, 293–298.CrossRefGoogle Scholar
Baskin, C.C., Baskin, J.M. and Chester, E.W. (2001) Morphophysiological dormancy in seeds of Chamaelirium luteum, a long-lived dioecious lily. Journal of the Torrey Botany Society 128, 7–15.CrossRefGoogle Scholar
Baskin, C.C., Baskin, J.M., Yoshinoga, A. and Thompson, K. (2005) Germination of drupelets in multi-seeded drupes of the shrub Leptecophylla tameiameiae (Ericaceae) from Hawaii: a case for deep physiological dormancy broken by high temperatures. Seed Science Research 15, 349–356.CrossRefGoogle Scholar
Baskin, C.C., Chien, C.T., Chen, S.Y. and Baskin, J.M. (2008) Germination of Viburnum odoratissimum seeds: a new level of morphophysiological dormancy. Seed Science Research 18, 179–184.CrossRefGoogle Scholar
Baskin, J.M. and Baskin, C.C. (1979) Promotion of germination of Stellaria media seeds by light from a green safe lamp. New Phytologist 82, 381–383.CrossRefGoogle Scholar
Baskin, J.M. and Baskin, C.C. (1984) Germination ecophysiology of the woodland herb Osmorhiza longistylis (Umbelliferae). American Journal of Botany 71, 687–692.CrossRefGoogle Scholar
Baskin, J.M. and Baskin, C.C. (1985) Seed germination ecophysiology of the woodland spring geophyte Erythronium albidum. Botanical Gazette 146, 130–136.CrossRefGoogle Scholar
Baskin, J.M. and Baskin, C.C. (1991) Nondeep complex morphophysiological dormancy in seeds of Osmorhiza claytonii (Apiaceae). American Journal of Botany 78, 588–593.CrossRefGoogle Scholar
Baskin, J.M. and Baskin, C.C. (2004) A classification system for seed dormancy. Seed Science Research 14, 1–16.CrossRefGoogle Scholar
Baskin, J.M. and Baskin, C.C. (2008) Some considerations for adoption of Nikolaeva's formula system into seed dormancy classification. Seed Science Research 18, 131–137.CrossRefGoogle Scholar
Bonde, E.K. (1965) Further studies on the germination of seeds of Colorado alpine plants. University of Colorado Studies Series in Biology 18, 1–30.Google Scholar
Burrows, C.J. (1993) Germination requirements of the seeds of native trees, shrubs and vines. Canterbury Botanical Society Journal 27, 42–48.Google Scholar
Burrows, C.J. (1996) Germination behaviour of the seeds of seven New Zealand vine species. New Zealand Journal of Botany 34, 93–102.CrossRefGoogle Scholar
Cochrane, A., Kelly, A., Brown, K. and Cunneen, S. (2002) Relationships between seed germination requirements and ecophysiological characteristics aid the recovery of threatened native plant species in Western Australia. Ecological Management and Restoration 3, 47–59.CrossRefGoogle Scholar
Copete, E., Herranz, J.M., Ferrandis, P., Baskin, C.C. and Baskin, J.M. (2011) Physiology, morphology and phenology of seed dormancy-break and germination in the endemic Iberian species Narcissus hispanicus (Amaryllidaceae). Annals of Botany 107, 1003–1016.CrossRefGoogle ScholarPubMed
Copete, M.A., Herranz, J.M. and Ferrandis, P. (2005) Seed dormancy and germination in threatened Iberian Coincya (Brassicaceae) taxa. Ecoscience 12, 257–266.CrossRefGoogle Scholar
Crocker, W. and Barton, L.V. (1957) Physiology of seeds. Waltham, Massachusetts, USA, Chronica Botanica Company.Google Scholar
Dafni, A., Shmida, A. and Avishai, M. (1981) Leafless autumnal-flowering geophytes in the Mediterranean region: phytogeographical, ecological and evolutionary aspects. Plant Systematics and Evolution 137, 181–193.CrossRefGoogle Scholar
D'Antuono, L.F. and Lovato, A. (2003) Germination trials and domestication potential of three native species with edible sprouts: Ruscus aculeatus L., Tamus communis L. and Smilax aspera L. Acta Horticulturae 598, 211–218.CrossRefGoogle Scholar
Emery, D.E. (1988) Seed propagation of native California plants. Santa Barbara, USA, Santa Barbara Botanic Garden.Google Scholar
Figueroa, J.A. (2003) Seed germination in temperate rain forest species of southern Chile: chilling and gap-dependency germination. Plant Ecology 166, 227–240.CrossRefGoogle Scholar
Frost-Christensen, H. (1974) Embryo development in ripe seeds of Eranthis hiemalis and its relation to gibberellic acid. Physiologia Plantarum 30, 200–205.CrossRefGoogle Scholar
Gómez, D., Azorín, J., Bastida, J., Viladomat, F. and Codina, C. (2003) Seasonal and spatial variations of alkaloids in Merendera montana in relation to chemical defense and phenology. Journal of Chemical Ecology 29, 1117–1126.CrossRefGoogle ScholarPubMed
Gómez-García, D., Azorín, J., Giannoni, S.M. and Borghi, C.E. (2004) How does Merendera montana (L.) Lange (Liliaceae) benefit from being consumed by mole-voles? Plant Ecology 172, 173–181.CrossRefGoogle Scholar
Gómez-García, D., Azorín, J. and Aguirre, J. (2009) Effects of small-scale disturbances and elevation on the morphology, phenology and reproduction of a successful geophyte. Journal of Plant Ecology 2, 13–20.CrossRefGoogle Scholar
Grime, J.P. (1981) The role of seed dormancy in vegetation dynamics. Annals of Applied Biology 98, 555–558.CrossRefGoogle Scholar
Hawkins, T.S., Baskin, J.M. and Baskin, C.C. (2007) Seed morphology, germination phenology, and capacity to form a seed bank in six herbaceous layer Apiaceae species of the Eastern Deciduous Forest. Castanea 72, 8–14.CrossRefGoogle Scholar
Herranz, J.M., Copete, M.A., Ferrandis, P. and Copete, E. (2010a) Intermediate complex morphophysiological dormancy in the endemic Iberian Aconitum napellus subsp. castellanum (Ranunculaceae). Seed Science Research 20, 109–121.CrossRefGoogle Scholar
Herranz, J.M., Ferrandis, P. and Martínez-Duro, E. (2010b) Seed germination ecology of the threatened endemic Iberian Delphinium fissum subsp. sordidum (Ranunculaceae). Plant Ecology 211, 89–106.CrossRefGoogle Scholar
Hidayati, S.N., Baskin, J.M. and Baskin, C.C. (2001) Dormancy-breaking and germination requirements for seeds of Symphoricarpos orbiculatus (Caprifoliaceae). American Journal of Botany 88, 1444–1451.CrossRefGoogle ScholarPubMed
Kondo, T., Okubo, N., Miura, T., Honda, K. and Ishikawa, Y. (2002) Ecophysiology of seed germination in Erythronium japonicum (Liliaceae) with underdeveloped embryos. American Journal of Botany 89, 1779–1784.CrossRefGoogle ScholarPubMed
Kondo, T., Miura, T., Okubo, N., Shimada, M., Baskin, C.C. and Baskin, J.M. (2004) Ecophysiology of deep simple epicotyl morphophysiological dormancy in seeds of Gagea lutea (Liliaceae). Seed Science Research 14, 371–378.CrossRefGoogle Scholar
Kondo, T., Sato, C., Baskin, J.M. and Baskin, C.C. (2006) Post-dispersal embryo development, germination phenology, and seed dormancy in Cardiocrinum cordatum var. glehnii (Liliaceae s. str.), a perennial herb of the broadleaved deciduous forest in Japan. American Journal of Botany 93, 849–859.CrossRefGoogle ScholarPubMed
Kondo, T., Mikubo, M., Yamada, K., Walck, J.L. and Hidayati, S.N. (2011) Seed dormancy in Trillium camschatcense (Melanthiaceae) and the possible roles of light and temperature requirements for seed germination in forests. American Journal of Botany 98, 215–226.CrossRefGoogle ScholarPubMed
Le Roux, L.G. and Robbertse, P.J. (1997) Aspects relating to seed production in Gloriosa superba L. South African Journal of Botany 63, 191–197.CrossRefGoogle Scholar
Liu, M., Li, R.-J. and Liu, M.-Y. (1993) Adaptive responses of roots and root systems to seasonal changes. Environmental and Experimental Botany 33, 175–188.CrossRefGoogle Scholar
Mabberley, D.J. (2008) Mabberley's plant-book. A portable dictionary of plants, their classification and uses (3rd edition). New York, Cambridge University Press.Google Scholar
MacMillan, B.H. (1972) Biological flora of New Zealand. 7. Rhipogonum scandens J. R. et G. Forst. (Smilacaceae) Supplejack, Kareao. New Zealand Journal of Botany 10, 641–672.CrossRefGoogle Scholar
McIntyre, S., Lavorel, S. and Trémont, R.M. (1995) Plant life-history attributes: their relationship to disturbance response in herbaceous vegetation. Journal of Ecology 83, 31–44.CrossRefGoogle Scholar
Moreno, J.C. and Sainz, M. (1992) Atlas corológico de las monocotiledóneas endémicas de la Península Ibérica e Islas Baleares. Madrid, Spain, ICONA.Google Scholar
Morgan, J.W. (1998) Comparative germination responses of 28 temperate grassland species. Australian Journal of Botany 46, 209–219.CrossRefGoogle Scholar
Narain, P. (1977) Morphological studies of some species and cultivars of Gloriosa. New Botany 4, 75–83.Google Scholar
Nikolaeva, M.G. (1969) Physiology of deep dormancy in seeds. Leningrad, Russia, Izdatel'stvo Nauka (translated from Russian to English in 1969 by Z. Shapiro, National Science Foundation, Washington, DC).Google Scholar
Nikolaeva, M.G. (1977) Factors controlling the seed dormancy pattern. pp. 51–74 in Khan, A.A. (Ed.) The physiology and biochemistry of seed dormancy and germination. Amsterdam, The Netherlands, North-Holland.Google Scholar
Nikolaeva, M.G. (2001) Ekologo-fiziologicheskie osobennosti pokoya i prorastaniya semyan (itogi issledovantii zaistekshee stoletie) [Ecological and physiological aspects of seed dormancy and germination (review of investigations for the last century)]. Botanicheskii Zhurnal 86, 1–14. (For a slightly modified version of the English translation, an update of Nikolaeva's seed dormancy classification system and its relevance to the ecology, physiology, biogeography and phylogenetic relationship of seed dormancy and germination, see website http://www.kew.org/isss/isss/Nikolaeva-manuscript-web.doc; last accessed in July 2011.).Google Scholar
Nikolaeva, M.G., Rasumova, M.V. and Gladkova, V.N. (1985) Reference book on dormant seed germination. Danilova, M.F. (Ed.). Leningrad, Nauka (in Russian).Google Scholar
Piotto, B. and De Noi, A. (2003) Seed propagation of Mediterranean trees and shrubs [Manuali e linee guida]. Agency for the Protection of the Environment and for Technical Services (APAT), Rome.Google Scholar
Pogge, F.L. and Bearce, B.C. (1989) Germinating common and cat greenbriar. Tree Planters Notes 40, 34–37.Google Scholar
Rosa, S.G.T. and Ferreira, A.G. (1999) Germination of medicinal plant: Smilax campestris Griseb. (Salsaparrilha). In Giberti, C.G.; Lorenz, L.; Mathe, M. (Eds) Proceedings of the Second World Congress on Medicinal and Aromatic Plants for Human Welfare. Agricultural Production, Post-harvest Techniques and Biotechnology. Acta Horticulturae 502, 105–111.CrossRefGoogle Scholar
Schiappacasse, F., Peñailillo, P., Yañez, P. and Bridgen, M. (2005) Propagation studies on Chilean geophytes. Acta Horticulturae 673, 121–126.CrossRefGoogle Scholar
Soltis, D.E., Soltis, P.S., Endress, P.K. and Chase, M.W. (2005) Phylogeny and evolution of angiosperms. Sunderland, USA, Sinauer Associates.Google Scholar
Stokes, P. (1953) The stimulation of growth by low temperature in embryos of Heracleum sphondylium L. Journal of Experimental Botany 4, 222–234.CrossRefGoogle Scholar
Stokes, P. (1965) Temperature and seed dormancy. pp. 746–803 in Ruhland, W. (Ed.) Encyclopedia of Plant Physiology, Vol. 15/2. New York, Springer-Verlag.Google Scholar
Takahashi, H. (1984) Germination ecology of Heloniopsis orientalis (Liliaceae). Science Reports of the Faculty of Education, Gifu University. Natural Science 8, 1–8.Google Scholar
Takhtajan, A. (1985) Anatomia seminum comparativa. Tomus 1. Liliopsida seu Monocotyledones. Leningrad, Nauka.Google Scholar
Threadgill, P.F., Baskin, J.M. and Baskin, C.C. (1981) Dormancy in seeds of Frasera caroliniensis. American Journal of Botany 68, 80–86.CrossRefGoogle Scholar
Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M. and Webb, D.A. (Eds) (1980) Flora Europaea. Alismataceae to Orchidaceae, Vol. 5. Cambridge, Cambridge University Press.Google Scholar
Vandelook, F., Bolle, N. and Van Assche, J.A. (2007) Seed dormancy and germination of the European Chaerophyllum temulum (Apiaceae), a member of a trans-Atlantic genus. Annals of Botany 100, 233–239.CrossRefGoogle ScholarPubMed
Vinnersten, A. and Reeves, G. (2003) Phylogenetic relationships within Colchicaceae. American Journal of Botany 90, 1455–1462.CrossRefGoogle ScholarPubMed
Walck, J.L., Baskin, J.M. and Baskin, C.C. (2000) Increased sensitivity to green light during transition from conditional dormancy to nondormancy in seeds of three species of Solidago (Asteraceae). Seed Science Research 10, 495–499.CrossRefGoogle Scholar
Whigham, D. (1974) An ecological life history study of Uvularia perfoliata L. The American Midland Naturalist 91, 343–359.CrossRefGoogle Scholar