Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T04:54:36.106Z Has data issue: false hasContentIssue false
  • English
  • Français

Manipulation of desiccation-sensitive axes of wampee (Clausena lansium) to facilitate increased dehydration tolerance

Published online by Cambridge University Press:  22 February 2007

J.R. Fu ,
X.M. Huang  and
S.Q. Songa
J.R. Fu*
Affiliation:
School of Life Science, Zhongshan University, Guangzhou 510275, People's Republic of China
X.M. Huang
Affiliation:
School of Life Science, Zhongshan University, Guangzhou 510275, People's Republic of China
S.Q. Songa
Affiliation:
School of Life Science, Zhongshan University, Guangzhou 510275, People's Republic of China
*
*Fax: +86-20-84036215 Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The plumules of newly-excised wampee embryos, which are more sensitive to dehydration than the roots, became more resistant to water loss when axes were allowed to sprout on woody plant medium [WPM; McCown and Lloyd (1981) Hortscience16, 453] before being dried. Pre-treatment of sprouting axes (seedlings) with sucrose incorporated in the WPM enhanced survival. Although the roots withered following further dehydration of seedlings cultured on WPM containing 60% sucrose, excised plumules were capable of generating adventitious roots when a combination of 10 mM α-napthaleneacetic acid and 10 mM indole-3-butyric acid was used during subsequent in vitro incubation.

Keywords

Axisdesiccation sensitivityrecalcitrant seedsucrose pre-treatmentswampee (Clausena lansium)woody plant medium
Type
Short Communication
Information
Seed Science Research , Volume 10 , Issue 3 , September 2000 , pp. 397 - 400
Copyright
Copyright © Cambridge University Press 2000

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Berjak, P., Farrant, J.M., Mycock, D.J. and Pammenter, N.W. (1990) Recalcitrant (homoiohydrous) seeds: the enigma of their desiccation-sensitivity. Seed Science and Technology 18, 297–310.Google Scholar
Berjak, P., Pammenter, N.W. and Vertucci, C. (1992) Homoiohydrous (recalcitrant) seeds: developmental status, desiccation sensitivity and the state of water in axes of Landolphia kirkii Dyer. Planta 186, 249–261.CrossRefGoogle ScholarPubMed
Crowe, J.H., Crowe, L.M. and Chapman, D. (1984) Preservation of membranes in anhydrobiotic organisms: the role of trehalose. Science 223, 701–703.CrossRefGoogle ScholarPubMed
Dumet, D. and Berjak, P. (1997) Desiccation tolerance and cryopreservation of embryonic axes of recalcitrant species. pp. 771–776in Ellis, R.H.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, Kluwer Academic Publishers.Google Scholar
Fu, J.R., Jin, J.P., Peng, Y.F. and Xia, Q.H. (1994) Desiccation tolerance in two species with recalcitrant seeds: Clausena lansium (Lour.) and Lichi chinensis (Sonn.). Seed Science Research 4, 257–261.CrossRefGoogle Scholar
Fu, J.R., Zhang, B.Z., Wang, X.F., Qiao, Y.Z. and Huang, X.L. (1989) Studies on desiccation and wet storage of four recalcitrant seeds. pp. 121–125in International symposium on horticultural germplasm of cultivated and wild fruit trees. Part 1. Beijing, International Academic Publishers.Google Scholar
Hoekstra, F.A., Crowe, L.M. and Crowe, J.H. (1989) Differential desiccation sensitivity of corn and Pennisetum pollen linked to their sucrose contents. Plant, Cell and Environment 12, 83–91.Google Scholar
Hoffmann, P. and Steiner, A.M. (1989) An updated list of recalcitrant seeds. Landwirtschafttiche Forschung 42, 310–323.Google Scholar
Leopold, A.C., Bruni, F. and Williams, R.T. (1992) Water in dry organisms. pp. 161–173in Somero, G.N.; Qsmond, C.B.; Bolis, C.L. (Eds) Water and life. Berlin, Springer-Verlag.Google Scholar
Lu, W.J. and Fu, J.R. (1997) The induction of desiccation tolerance in wampee (Clausena lansium Skeels) embryonic axes. Acta Scientiarum Naturalium Universitatis Sunyatseni 36, 116–120.Google Scholar
McCown, B.H. and Lloyd, G. (1981) Wood plant medium (WPM)-A mineral nutrient formulation for microculture of woody plant species. Hortscience 16, 453.Google Scholar
Obendorf, R.L. (1997) Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance. Seed Science Research 7, 63–74.CrossRefGoogle Scholar
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.Google Scholar
Roberts, E.H., King, M.W. and Ellis, R.H. (1984) Recalcitrant seeds: their recognition and storage. pp. 38–52in Holden, J.A.; Williams, J.T. (Eds) Crop genetic resources: conservation and evaluation. London, George Allen & Unwin.Google Scholar
Song, S.Q. and Fu, J.R. (1997) Desiccation-sensitivity and lipid peroxidation in chinese wampee (Clausena lansium [Lour.] Skeels) seeds. Acta Phytophysiologica Sinica 23, 163–168.Google Scholar
Uragami, A., Lucas, M.O., Ralambosoa, J., Renard, M. and Dereuddre, J. (1993) Cryopreservation of microspore embryos of oilseed rape (Brassica napus L.) by dehydration in air with or without alginate encapsulation. Cryo-Letters 14, 83–90.Google Scholar
Xiang, X. and Fu, J.R. (1997) The ways to increase vigor of wampee (Clausena lansium) seeds. Journal of Tropical and Subtropical Botany 5, 39–44.Google Scholar
Yap, L.V., Hor, Y.L. and Normah, M.N. (1999) Effects of sucrose preculture and subsequent desiccation on cryopreservation of alginate-encapsulated Hevea brasiliensis embryos. pp. 140–143in Marzalina, M.; Khoo, K.C.; Jayanthi, N.; Tsan, F.Y.; Krishnapillay, B. (Eds) IUFRO seed symposium 1998. Recalcitrant seeds. Kuala Lumpur, FRIM.Google Scholar