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Heat-shock proteins in monitoring aging and heat-induced tolerance in germinating wheat and barley embryos

Published online by Cambridge University Press:  19 September 2008

A. Dell'Aquila ,
M. G. Corona  and
M. Di Turi
A. Dell'Aquila*
Affiliation:
Germplasm Institute, CNR, 70122 Bari, Italy
M. G. Corona
Affiliation:
Germplasm Institute, CNR, 70122 Bari, Italy
M. Di Turi
Affiliation:
Germplasm Institute, CNR, 70122 Bari, Italy
*
*Correspondence [email protected]+ 39 80 5587566
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Abstract

Wheat and barley seeds were subjected to accelerated aging conditions at 12% moisture content and 35°C temperature over 28 days of storage, and to heat-shock treatment carried out by 4 h of incubation at 40°C following 16 h of imbibition at 20°C. Heat-treated, aged seeds showed altered germination behaviour and increasing leakage electroconductivity as well as a reduced incorporation of [35S]-methionine into embryo proteins, in comparison with the corresponding untreated seeds. Two-dimensional electrophoresis of labelled proteins from embryos gave evidence of further quantitative and qualitative changes: (a) ‘normal’ germination protein synthesis in wheat and barley was slightly modified by age, but following heat-shock treatment a general reduction of most of the control polypeptides occurred; (b) heat-shock response resulted in the production of several HSPs with different MW and pl, but a uniform general decline in their synthesis was not observed. Highly labelled HSPs (e.g., those with MW 66.7 or 89.3–66.7 kDa in wheat or barley, respectively) did not change over the entire period of aging, while some smaller HSPs with MW >29 kDa either decreased in intensity or disappeared. Conversely, specific low molecular weight HSPs (MW 17–14.2 kDa) were synthesized more in extremely aged embryos of both species. The hypothesis that these polypeptides may be used as biochemical markers in monitoring both vigour loss and heat-shock induced tolerance in aged seeds is discussed.

Keywords

Triticum durumHordeum vulgareaginggerminationheat-shockprotein synthesis
Type
Research Papers
Information
Seed Science Research , Volume 8 , Issue 2 , June 1998 , pp. 91 - 98
Copyright
Copyright © Cambridge University Press 1998

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References

Abernethy, R H, Thiel, D S, Petersen, N S and Helm, H (1989) Thermotolerance is developmentally dependent in germinating wheat seed. Plant Physiology 89, 576–596.CrossRefGoogle ScholarPubMed
Bewley, J D and Black, M (1994) Seeds. Physiology of development and germination. pp 230–268. New York, Plenum Press.CrossRefGoogle Scholar
Bewley, J D and Marcus, A (1990) Gene expression in seed development and germination. pp 165–193in Cohn, W E and Moldave, K (Eds) Progress in nucleic acid research and molecular biology. 38, San Diego, Academic Press, Inc.Google Scholar
Dell'Aquila, A (1994) Wheat seed ageing and embryo protein degradation. Seed Science Research. 4, 293–298.CrossRefGoogle Scholar
Dell'Aquila, A and Di Turi, M (1995) Deterioration of wheat seeds under subfreezing temperatures and related protein synthesis in germinating embryos. Seed Science and Technology 23, 551–561.Google Scholar
Dell'Aquila, A and Di Turi, M (1996) The germination response to heat and salt stress in evaluating vigour loss in aged wheat seeds. Seed Science and Technology 24, 309–319.Google Scholar
Dell'Aquila, A and Margiotta, B (1986) DNA synthesis and mitotic activity in germinating wheat seeds aged under various conditions. Environmental and Experimental Botany 26, 175–184.CrossRefGoogle Scholar
Dell'Aquila, A and Spada, P (1992) Regulation of protein synthesis in germinating wheat embryos under polyethylene glycol and salt stress. Seed Science Research 2, 75–80.CrossRefGoogle Scholar
Dell'Aquila, A and Spada, P (1994) Effect of low and high temperatures on protein synthesis patterns of germinating wheat embryos. Plant Physiology and Biochemistry 32, 65–73.Google Scholar
Ellis, R H and Roberts, E H (1981) The quantification of ageing and survival in orthodox seeds. Seed Science and Technology 9, 373–409.Google Scholar
Helm, K W, Petersen, N S and Abernethy, R H (1989) Heat shock response of germinating embryos of wheat. Plant Physiology 90, 598–605.CrossRefGoogle ScholarPubMed
Hong, T D and Ellis, R H (1992) Development of desiccation tolerance in Norway maple (Acer platanoides L.) seeds during maturation drying. Seed Science Research 2, 155–172.CrossRefGoogle Scholar
Howarth, C (1989) Heat shock proteins in Sorghum bicolor and Pennisetum americanum I. Genotypic and developmental variation during seed germination. Plant, Cell and Environment 12, 471–477.CrossRefGoogle Scholar
Hsieh, M-H., Chen, J-T., Jinn, T-L., Chen, Y-M. and Lin, C-Y. (1992) A class of soybean low molecular weight heat shock proteins. Plant Physiology 99, 1279–1284.CrossRefGoogle ScholarPubMed
ISTA (1993) International rules for seed testing. Seed Science and Technology 21, Supplement, 1–75.Google Scholar
Laemmli, U K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Laskey, R A and Mills, A D (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56, 335–341.CrossRefGoogle ScholarPubMed
Priestley, D A (1986) Morphological, structural and biochemical changes associated with seed aging. pp 125–195in Priestley, D A (Ed.) Seed aging. Ithaca, London, Comstock Publishing Associates.Google Scholar
Sulc, R M, Albretch, K A and Duke, S H (1991) Leakage of intracellular substances as an indicator of freezing injury in Alfalfa. Crop Science 31, 430–435.CrossRefGoogle Scholar
Tadmor, N H, Cohen, Y and Harpaz, Y (1969) Interactive effects of temperature and osmotic potential on the germination of range plants. Crop Science 9, 771–774.CrossRefGoogle Scholar
Thompson, E W and Lane, B G (1980) Relation of protein synthesis in imbibing wheat embryos to the cell-free translational capacities of bulk mRNA from dry and imbibing embryos. Journal of Biological Chemistry 255, 5965–5970.CrossRefGoogle Scholar
Van De Venter, H A, Barla-Szabo, G and Ybema, S G (1993) A study of single and multiple stress seed vigour tests for undeteriorated seed lots of wheat. Seed Science and Technology 21, 117–125.Google Scholar
Vierling, E (1991) The roles of heat shock proteins in plants. Annual Review of Plant Physiology and Plant Molecular Biology 42, 579–620.CrossRefGoogle Scholar
Wechsberg, G E, Probert, R J and Bray, C M (1994) The relationship between ‘dehydrin-like’ proteins and seed longevity in Ranunculus sceleratus L. Journal of Experimental Botany 45, 1027–1030.CrossRefGoogle Scholar