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Evidence of different compounds in smoke derived from legumes and grasses acting on seed germination and seedling emergence

Published online by Cambridge University Press:  19 April 2017

Lei Ren ,
Yuguang Bai  and
Martin Reaney
Lei Ren
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, Canada
Yuguang Bai*
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, Canada
Martin Reaney
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, Canada
*
*Correspondence E-mail: [email protected]
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Abstract

Our previous study showed that smoke derived from alfalfa (Medicago sativa) caused different germination responses compared with that from prairie hay (Festuca hallii) and wheat straw (Triticum aestivum), but the mechanism remained unclear. In this study, we used Salad Bowl lettuce (Lactuca sativa) as a quick bioassay to trace the active compounds in each of these three smoke solutions. Column chromatography and high performance liquid chromatography (HPLC) were used to separate and identify active fractions. Seeds of four species from Fescue Prairie were primed for 24 h at room temperature in darkness using serial dilutions of separated active fractions, as well as karrikinolide (KAR1). After priming, seeds were dried at room temperature in darkness for 7 days and subsequently incubated at 10/0°C or 25/15°C in 12 h light–12 h dark or 24 h darkness for 49 days. KAR1 was in the smoke made from prairie hay, and wheat straw, but was absent in alfalfa smoke. Priming in KAR1 solutions increased germination of three native species. Priming in highly concentrated KAR1 reduced radicle length of Cirsium arvense, the only non-native species. Even though KAR1 has the potential to enhance regeneration of native species in the Fescue Prairie, KAR1 is not universally present in smoke derived from different plant materials. Unknown compound(s) in smoke derived from legumes remain to be identified.

Keywords

Festuca halliigerminationkarrikinolideMedicago sativaseedling growthTriticum aestivum
Type
Research Papers
Information
Seed Science Research , Volume 27 , Special Issue 2: Seed Ecology , June 2017 , pp. 154 - 164
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Copyright
Copyright © Cambridge University Press 2017 

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References

Abu, Y., Romo, J.T., Bai, Y. and Coulman, B. (2016) Priming seeds in aqueous smoke solutions to improve seed germination and biomass production of perennial forage species. Canadian Journal of Plant Sciences 96, 551–563.Google Scholar
Anderson, H.G. and Bailey, A.W. (1980) Effects of annual burning on grassland in the Aspen parkland of east-central Alberta. Canadian Journal of Botany 58, 985–996.Google Scholar
Aran, D., Garcia-Duro, J., Reyes, O. and Casal, M. (2013) Fire and invasive species: Modifications in the germination potential of Acacia melanoxylon, Conyza canadensis and Eucalyptus globulus . Forest Ecology and Management 302, 7–13.CrossRefGoogle Scholar
Bailey, A.W. and Anderson, H.G. (1978) Prescribed burning of a Festuca-Stipa grassland. Journal of Range Management 31, 446–449.Google Scholar
Baxter, B., Granger, J. and Van Staden, J. (1995) Plant-derived smoke and seed germination: is all smoke good smoke? That is the burning question. South African Journal of Botany 61, 275–277.Google Scholar
Brits, G.J., Calitz, F.L., Brown, N.A.C. and Manning, J.C. (1993) Desiccation as the active principle in heat-stimulated seed germination of Leucospermum R. Br. (Proteaceae) in fynbos. New Phytologist 125, 397–403.Google Scholar
Brown, N.A.C. (1993) Promotion of germination of fynbos seeds by plant-derived smoke. New Phytologist 123, 575–583.CrossRefGoogle ScholarPubMed
Catav, S.S., Bekar, I., Ates, B.S., Ergan, G., Oymak, F., Ulker, E.D. and Tavsanoglu, C. (2012) Germination response of five eastern Mediterranean woody species to smoke solution derived from various plant. Turkish Journal of Botany 36, 480–487.Google Scholar
Collins, S.L. (1987) Interaction of disturbances in tallgrass prairie: a field experiment. Ecology 68, 1243–1250.CrossRefGoogle Scholar
Commander, L.E., Merritt, D.J., Rokich, D.P. and Dixon, K.W. (2009) Seed biology of Australian arid zone species: germination of 18 species used for rehabilitation. Journal of Arid Environments 73, 617–625.Google Scholar
Daws, M.I., Davies, J., Pritchard, H.W., Brown, N.A.C. and Van Staden, J. (2007) Butenolide from plant-derived smoke enhances germination and seedling growth of arable weed species. Plant Growth Regulation 51, 73–82.Google Scholar
De Lange, J.H. and Boucher, C. (1990) Autecological studies on Audouinia capitata Bruniaceae I. Plant-derived smoke as a seed germination cue. South African Journal of Botany 56, 700–703.Google Scholar
Dixon, K.W., Roche, S. and Pate, J.S. (1995) The promotive effect of smoke derived from burnt native vegetation on seed germination of Western Australian plants. Oecologia 101, 185–192.CrossRefGoogle ScholarPubMed
DiTomaso, J.M., Kyser, G.B. and Hastings, M.S. (1999) Prescribed burning for control of yellow starthistle (Centaurea solstitialis) and enhanced native plant diversity. Weed Science 47, 233–242.Google Scholar
Downes, K.S., Lamont, B.B., Light, M.E. and Van Staden, J. (2010) The fire ephemeral Tersonia cyathiflora (Gyrostemonaceae) germinates in response to smoke but not the butenolide 3-methyl-2H-furo[2,3-c] pyran-2-one. Annals of Botany 106, 381–384.CrossRefGoogle Scholar
Drewes, F., Smith, M. and Staden, J. (1995) The effect of a plant-derived smoke extract on the germination of light-sensitive lettuce seed. Plant Growth Regulation 16, 205–209.Google Scholar
Environment Canada (2012) Daily data report (http://climate.weather.gc.ca/climate_data/daily_data_e.html?timeframe=2&hlyRange=2008-12-02%7C2017-04-04&dlyRange=2008-12-02%7C2017-04-02&mlyRange=%7C&StationID=47707&Prov=SK&urlExtension=_e.html&searchType=stnName&optLimit=yearRange&StartYear=1840&EndYear=2017&selRowPerPage=25&Line=12&searchMethod=contains&txtStationName=Saskatoon&Day=4&Year=2012&Month=4#) (accessed 15 March 2013).Google Scholar
Flematti, G.R., Ghisalberti, E.L., Dixon, K.W. and Trengove, R.D. (2004) A compound from smoke that promotes seed germination. Science 305, 977–977.Google Scholar
Flematti, G.R., Ghisalberti, E.L., Dixon, K.W. and Trengove, R.D. (2009) Identification of alkyl substituted 2H-Furo[2,3-c] pyran-2-ones as germination stimulants present in smoke. Journal of Agricultural and Food Chemistry 57, 9475–9480.Google Scholar
Flematti, G.R., Ghisalberti, E.L., Dixon, K.W. and Trengove, R.D. (2008) Germination stimulant in smoke: isolation and identification. In Colegate, S.M. and Molyneux, R.J. (eds), Bioactive Natural Products: Detection, Isolation and Structural Determination, pp. 531–544. Boca Raton, CRC Press.Google Scholar
Flematti, G.R., Merritt, D.J., Piggott, M.J., Trengove, R.D., Smith, S.M., Dixon, K.W. and Ghisalberti, E.L. (2011) Burning vegetation produces cyanohydrins that liberate cyanide and stimulate seed germination. Nature Communications 2, 360.CrossRefGoogle ScholarPubMed
Gardner, M.J., Dalling, K.J. and Light, M.E. (2001) Does smoke substitute for red light in the germination of light-sensitive lettuce seeds by affecting gibberellin metabolism? South African Journal of Botany 67, 636–640.Google Scholar
Ghebrehiwot, H.M., Kulkarni, G. and Kirkman, K.P. (2009) Smoke solutions and temperature influence the germination and seedling growth of South African mesic grassland species. Rangeland Ecology and Management 62, 572–578.Google Scholar
Henig-Sever, N., Eshel, A. and Ne'eman, G. (1996) pH and osmotic potential of pine ash as post-fire germination inhibitors. Physiologia Plantarum 96, 71–76.Google Scholar
Jager, A.K., Light, M.E. and Van Staden, J. (1996) Effects of source of plant material and temperature on the production of smoke extracts that promote germination of light-sensitive lettuce seeds. Environmental and Experimental Botany 36, 421–429.Google Scholar
Jain, N., Kulkarni, M.G. and Van Staden, J. (2006) A butenolide, isolated from smoke, can overcome the detrimental effects of extreme temperatures during tomato seed germination. Plant Growth Regulation 49, 263–267.Google Scholar
Keeley, J.E. and Fotheringham, C.J. (1997) Trace gas emissions and smoke-induced seed germination. Science 276, 1248–1250.CrossRefGoogle Scholar
Kulkarni, M.G., Sparg, S.G. and Van Staden, J. (2007) Germination and post-germination response of Acacia seeds to smoke-water and butenolide, a smoke-derived compound. Journal of Arid Environments 69, 177–187.Google Scholar
MacDonald, N.W., Scull, B.T. and Abella, S.R. (2007) Mid-spring burning reduces spotted knapweed and increases native grasses during a Michigan experimental grassland establishment. Restoration Ecology 15, 118–128.Google Scholar
Merritt, D.J., Kristiansen, M., Flematti, G.R., Turner, S.R., Ghisalberti, E.L., Trengove, R.D. and Dixon, K.W. (2006) Effects of a butenolide present in smoke on light-mediated germination of Australian Asteraceae . Seed Science Research 16, 29–35.Google Scholar
Musil, C.F. and de Witt, D.W. (1991) Heat-stimulated germination in two Restionaceae species. South African Journal of Botany 57, 175–176.CrossRefGoogle Scholar
Nelson, D.C., Riseborough, J., Flematti, G.R., Stevens, J., Ghisalberti, E.L., Dixon, K.W. and Smith, S.M. (2009) Karrikins discovered in smoke trigger Arabidopsis seed germination by a mechanism requiring gibberellic acid synthesis and light. Plant Physiology 149, 863–873.Google Scholar
Perez-Fernandez, M.A. and Rodriguez-Echeverria, S. (2003) Effect of smoke, charred wood, and nitrogenous compounds on seed germination of ten species from woodland in Central-Western Spain. Journal of Chemical Ecology 29, 237–251.Google Scholar
Prober, S.M., Thiele, K.R., Lunt, I.D. and Koen, T.B. (2005) Exotic annuals and native perennial grasses through carbon supplements and spring burns. Journal of Applied Ecology 42, 1073–1085.Google Scholar
Ren, L. and Bai, Y.G. (2016 a) Burning modifies composition of emergent seedlings in Fescue Prairie. Rangeland Ecology and Management 70, 230–237.Google Scholar
Ren, L. and Bai, Y.G. (2016 b) Smoke and ash effects on seedling emergence from germinable soil seed bank in fescue prairie. Rangeland Ecology and Management 69, 499–507.Google Scholar
Ren, L. and Bai, Y.G. (2016 c) Smoke originated from different plants has various effects on germination and seedling growth of species in Fescue Prairie. Botany 94, 1141–1150.Google Scholar
Romo, J.T. and Gross, D.V. (2011) Preburn history and seasonal burning effects on the soil seed bank in the Fescue Prairie. American Midland Naturalist 165, 74–90.Google Scholar
Schwachtje, J. and Baldwin, I.T. (2004) Smoke exposure alters endogenous gibberellin and abscisic acid pools and gibberellin sensitivity while eliciting germination in the post-fire annual, Nicotiana attenuate . Seed Science Research 14, 51–60.CrossRefGoogle Scholar
Smith, C.J., Perfetti, T.A., Garg, R. and Hansch, C. (2003) IARC carcinogens reported in cigarette mainstream smoke and their calculated log P values. Food and Chemical Toxicology 41, 807–817.Google Scholar
Stevens, J.C., Merritt, D.J., Flematti, G.R., Ghisalberti, E.L. and Dixon, K.W. (2007) Seed germination of agriculture weeds is promoted by the butenolide 3-methyl-2H-furo[2,3-c] pyran-2-one under laboratory and field conditions. Plant Soil 298, 113–124.Google Scholar
Thomas, P.B., Morris, E.C., Auld, T.D. and Haigh, A.M. (2010) The interaction of temperature, water availability and fire cues regulates seed germination in a fire-prone landscape. Oecologia 162, 293–302.Google Scholar
Van Staden, J., Brown, N.A.C., Jäger, A.K. and Johnson, T.A. (2000) Smoke as a germination cue. Plant Species Biology 15, 167–178.Google Scholar
Van Staden, J., Jager, A.K., Light, M.E. and Burger, B.V. (2004) Isolation of the major germination cue from plant-derived smoke. South African Journal of Botany 70, 654–659.Google Scholar
Van De Venter, H.A. and Esterhuizen, A.D. (1988) The effects of factors associated with fire on seed germination of Erica sessiliflora and E . herbacalyx (Ericaceae). South African Journal of Botany 54, 301–304.Google Scholar
Wilson, R.G. (1982) Germination and seedling development of fringed sagebrush (Artemisia frigida). Weed Science 30, 102–105.CrossRefGoogle Scholar