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Does benzyladenine stimulate DNA metabolism by modifying gene expression during maize germination?

Published online by Cambridge University Press:  19 September 2008

José Juan Zúñiga-Aguilar ,
Imelda López ,
Alberto Gómez  and
Jorge M. Vázquez-Ramos
José Juan Zúñiga-Aguilar
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México 04510, D.F., México
Imelda López
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México 04510, D.F., México
Alberto Gómez
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México 04510, D.F., México
Jorge M. Vázquez-Ramos*
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México 04510, D.F., México
*
*Correspondence
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Abstract

Benzyladenine, a synthetic cytokinin, stimulates DNA metabolism during maize germination; apparently such stimulation occurs in two phases, and during the first phase stimulations of DNA synthesis, of protein kinase activity (both α-amanitin repressible), of DNA polymerase and poly ADP-ribosyl polymerase activities are observed. Since the evidence suggested that the effects produced by BA were due, at least partially, to transcriptional activation, attempts were made to discover genes, by subtractive hybridization of a cDNA library or by differential display, whose expression depended on the presence of BA; however these were unsuccessful. BA modulated the expression of a limited number of genes only. The possible mechanism by which BA could be promoting DNA metabolism during maize germination is discussed.

Keywords

maizeseed germinationbenzyladenineDNA metabolism
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 5 , Issue 4 , December 1995 , pp. 219 - 226
Copyright
Copyright © Cambridge University Press 1995

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References

Binns, A.N. (1994) Cytokinin accumulation and action: biochemical, genetic and molecular approaches. Annual Review Plant Physiology and Plant Molecular Biology 45, 173–196.Google Scholar
Bradford, M. (1976) A refined and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–252.Google Scholar
Cheah, K.S.E. and Osborne, D.J. (1978) DNA lesions occur with loss of viability in embryos of ageing rye seed. Nature 272, 593–599.CrossRefGoogle ScholarPubMed
Chomczynski, P. (1993) A reagent for the single-step isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15, 532–536.Google ScholarPubMed
Coello, P. and Vázquez-Ramos, J.M. (1995) Maize DNA polymerase 2 is a phosphoprotein with increasing activity during germination. European Journal of Biochemistry 231(1), 99–103.Google Scholar
Elder, R.H. and Osborne, D.J. (1993) Function of DNA synthesis and DNA repair in the survival of embryos during early germination and in dormancy. Seed Science Research 3, 43–53.CrossRefGoogle Scholar
Fletcher, R.A. (1969) Retardation of leaf senescence by BA in intact bean plants. Planta 89, 1–8.Google Scholar
Galli, M.G. (1984) Synthesis of DNA in excised watermelon cotyledons grown in water and BA. Planta 160, 193–199.CrossRefGoogle Scholar
Grey, J.E. and Bryant, J.A. (1984) Changes in the activity of Poly(Adenosine Diphosphate-ribose) polymerase during germination of pea. Phytochemistry 23, 477–478.CrossRefGoogle Scholar
Liang, P. and Pardee, A.B. (1992) Differential display of euka-ryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967–971.CrossRefGoogle ScholarPubMed
Longo, G.P.M., Bracale, M., Rossi, G. and Longo, C.P. (1990) BA induces the appearance of LHCP-mRNA and of the relevant protein in dark-grown excised watermelon cotyledons. Plant Molecular Biology 14, 569–573.CrossRefGoogle ScholarPubMed
Lu, J.L., Ertl, J.R. and Chen, C.M. (1990) Cytokinin enhancement of the light induction of nitrate reductase transcript levels in etiolated barley leaves. Plant Molecular Biology 14, 585–594.Google Scholar
Meléndez, S. and Vázquez-Ramos, J.M. (1990) Estudios sobre la naturaleza y localización de la sintesis de ADN durante las etapas iniciales de la germinación de maíz. Revista de la Sociedad Quimica de México 34, 354–359.Google Scholar
Murray, A.W. and Kirschner, M.W. (1989) Dominos and clocks: the union of two views of the cell cycle. Science 246, 614–621.Google Scholar
Naito, K., Tsuji, H. and Hatekeyama, I. (1978) Effect of BA on DNA, RNA, protein, and chlorophyll contents in intact bean leaves: differential responses to BA according to leaf age. Physiologia Plantarum 43, 367–371.CrossRefGoogle Scholar
Osborne, D.J. (1983) Biochemical control systems operating in the early hours of germination. Canadian Journal of Botany 61, 3568–3577.CrossRefGoogle Scholar
Osborne, D.J., Dell'Aquila, A. and Elder, R.H. (1984) DNA repair in plant cells. An essential event of early embryo germination in seeds. Folia Biologica (Praha) 30, 155–169.Google ScholarPubMed
Ohya, T. and Suzuki, H. (1991) The effects of BA on the accumulation of messenger RNAs that encode the large and small subunits of RubisCO and LHCPa/b protein in excised cucumber cotyledons. Plant and Cell Physiology 32, 577–580.Google Scholar
Reyes-Jiménez, J., Jiménez-García, L.F., González, M.A. and Vázquez-Ramos, J.M. (1991) Benzyladenine-stimulation of nuclear DNA synthesis and cell division in germinating maize. Seed Science Research 1, 113–117.Google Scholar
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning, a laboratory manual, Second Ed. Cold Spring Harbor Laboratory Press, New York, USA.Google Scholar
Satoh, M.S. and Lindahl, T. (1992) Role of Poly(ADP-ribose) formation in DNA repair. Nature 356, 356–358.CrossRefGoogle ScholarPubMed
Seyer, P. and Lescure, A.M. (1984) Evidence for changes in plastid mRNA populations during cytokinin induced chloroplast differentiation in tobacco cell suspensions. Plant Science Letters 36, 59–66.CrossRefGoogle Scholar
Skoog, F. and Miller, D.O. (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposium of the Society of Experimental Biology 11, 118–131.Google Scholar
Van Staden, J. (1983) Seeds and cytokinins. Physiologia Plantarum 55, 60–72.Google Scholar
Vázquez-Ramos, J.M. and Reyes, J. (1990) Stimulation of DNA synthesis and DNA polymerase activity during early germination of maize embryo axes. Canadian Journal of Botany 45, 649–653.Google Scholar
Yokoyama, M., Naito, K. and Suzuki, H. (1981) BA-enhanced cell proliferation and suppressed greening in attached young bean leaves. Plant and Cell Physiology 22, 623–627.Google Scholar
Zaraín, H.M., Bernal-Lugo, I. and Vázquez-Ramos, J.M. (1987) Effect of benzyladenine on the DNA synthesis during early germination of maize embryo axes. Mutation Research 181, 103–110.Google Scholar
Zwar, J.A. (1973) Effects of cytokinins on the nucleic acids of tobacco pith. Journal of Experimental Botany 24, 701–710.Google Scholar