Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-23T12:36:39.396Z Has data issue: false hasContentIssue false
  • English
  • Français

Changes in soluble sugars in relation to desiccation tolerance in cauliflower seeds

Published online by Cambridge University Press:  19 September 2008

F. A. Hoekstra ,
A. M. Haigh ,
F. A. A. Tetteroo  and
T. van Roekel
F. A. Hoekstra*
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
A. M. Haigh
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands Royal Sluis, Westeinde 161, 1601 BM Enkhuizen, Netherlands
F. A. A. Tetteroo
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands Royal Sluis, Westeinde 161, 1601 BM Enkhuizen, Netherlands
T. van Roekel
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
*
* Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

Changes in soluble sugars in cauliflower seeds were followed during 50 h of imbibition in relation to desiccation tolerance. Sucrose and stachyose contents decreased, and glucose and fructose accumulated. This occurred in radicles first and subsequently in hypocotyls and cotyledons. Loss of desiccation tolerance in the various seed parts coincided with an increase in glucose and fructose and the complete loss of stachyose, but sucrose content, the major sugar, was still high. Drying imbibed seeds over silica gel did not evoke resynthesis of stachyose, but did increase sucrose and decrease glucose and fructose contents. Seeds primed in solutions of 30% polyethylene glycol for 10 days showed a loss of stachyose, while sucrose remained high and glucose and fructose contents were still very low. Redrying of primed seeds did not change the sugar contents. The primed seeds were still tolerant of desiccation. We conclude that stachyose is not a prerequisite for desiccation tolerance, but that sucrose may be. We suggest that glucose and fructose may be involved in desiccation damage.

Keywords

Brassica oleracea var oleraceadesiccation tolerancedisaccharidesgerminationimbibitionmonosaccharidesoligosaccharidespolyethylene glycolpriming
Type
Short Communication
Information
Seed Science Research , Volume 4 , Issue 2 , June 1994 , pp. 143 - 147
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

Present address: Faculty of Horticulture, University of Western Sydney, Hawkesbury, Richmond NSW 2753, Australia.

References

Amuti, K.S. and Pollard, C.J. (1977) Soluble carbohydrates of dry and developing seeds. Phytochemistry 16, 529–532.Google Scholar
Bruni, F. and Leopold, A.C. (1991) Glass transitions in soybean seed. Plant Physiology 96, 660–663.CrossRefGoogle ScholarPubMed
Bruni, F. and Leopold, A.C. (1992) Cytoplasmic glass formation in maize embryos. Seed Science Research 2, 251–253.CrossRefGoogle Scholar
Crowe, L.M., Mouradian, R., Crowe, J.H., Jackson, S.A. and Womersley, C. (1984) Effects of carbohydrates on membrane stability at low water activities. Biochimica et Biophysica Acta 769, 141–150.CrossRefGoogle ScholarPubMed
Crowe, L.M., Crowe, J.H. and Chapman, D. (1985) Interaction of carbohydrates with dry dipalmitoyl-phosphatidylcholine. Archives of Biochemistry and Biophysics 236, 289–296.Google Scholar
Crowe, L.M., Womersley, C., Crowe, J.H., Reid, D., Appel, L. and Rudolph, A. (1986) Prevention of fusion and leakage in freeze-dried liposomes by carbohydrates. Biochimica et Biophysica Acta 861, 131–140.CrossRefGoogle Scholar
Crowe, J.H., Crowe, L.M., Carpenter, J.F. and Aurell Wistrom, C. (1987) Stabilization of dry phospholipid bilayers and proteins by sugars. Biochemical Journal 242, 1–10.Google Scholar
Crowe, J.H., Crowe, L.M., Carpenter, J.F., Rudolph, A.S., Aurell Wistrom, C., Spargo, B.J. and Anchordoguy, T.J. (1988) Interactions of sugars with membranes. Biochimica et Biophysica Acta 947, 367–384.Google Scholar
Crowe, J.H., Crowe, L.M., Carpenter, J.F., Prestrelski, S. and Hoekstra, F.A. (in press) Anhydrobiosis: cellular adaptations to extreme dehydration. In Dantzler, W. (Ed.) Handbook of physiology. Oxford, Oxford University Press.Google Scholar
DeAngelis, J.D., Berry, R.E. and Krantz, G.W. (1983) Evidence for the spider mite (Acari: Tetranychidae) injury-induced leaf water deficits and osmotic adjustment in peppermint. Environmental Entomology 12, 336–339.CrossRefGoogle Scholar
Hoekstra, F.A. and van Roekel, T. (1988) Desiccation tolerance of Papaver dubium L. pollen during its development in the anther: possible role of phospholipid composition and sucrose content. Plant Physiology 88, 626–632.Google Scholar
Koster, K.L. (1991) Glass formation and desiccation tolerance in seeds. Plant Physiology 96, 302–304.CrossRefGoogle ScholarPubMed
Koster, K.L. and Leopold, A.C. (1988) Sugars and desiccation tolerance in seeds. Plant Physiology 88, 829–832.CrossRefGoogle ScholarPubMed
Leprince, O. (1992) Etude des mécanismes de la résistance à la déshydratation dans les embryons de plantes supérieures. PhD Thesis, University of Liège, Belgium.Google Scholar
Leprince, O., van der Werf, A., Deltour, R. and Lambers, H. (1992) Respiratory pathways in germinating maize radicles correlated with desiccation tolerance and soluble sugars. Physiologia Plantarum 85, 581–588.CrossRefGoogle Scholar
Mouradian, R., Womersley, C., Crowe, L.M. and Crowe, J.H. (1984) Preservation of functional integrity during long term storage of a biological membrane. Biochimica et Biophysica Acta 778, 615–617.CrossRefGoogle ScholarPubMed
Tarquis, A.M. and Bradford, K.J. (1992) Prehydration and priming treatments that advance germination also increase the rate of deterioration of lettuce seeds. Journal of Experimental Botany 43, 307–317.Google Scholar
Tetteroo, F.A.A., Bomal, C., Hoekstra, F.A. and Karssen, C.M. (1994) Effect of abscisic acid and slow drying on soluble carbohydrate content of developing embryoids of carrot (Daucus carota L.) and alfalfa (Medicago sativa L.). Seed Science Research 4, 203–210.CrossRefGoogle Scholar
Virgona, J.M. and Barlow, E.W.R. (1991) Drought stress induces changes in the nonstructural carbohydrate composition of wheat stems. Australian Journal of Plant Physiology 18, 239–247.Google Scholar
Wettlaufer, S.H. and Leopold, A.C. (1991) Relevance of Amadori and Maillard products to seed deterioration. Plant Physiology 97, 165–169.CrossRefGoogle ScholarPubMed