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Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming

Published online by Cambridge University Press:  22 February 2007

Christophe Bailly ,
Abdelilah Benamar ,
Françoise Corbineau  and
Daniel Côme
Christophe Bailly
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, tour 53, 1er étage, 4 place Jussieu, 75252 Paris cédex 05, France
Abdelilah Benamar
Affiliation:
LRPV, 16 boulevard Lavoisier, 49045 Angers cédex 01, France
Françoise Corbineau*
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, tour 53, 1er étage, 4 place Jussieu, 75252 Paris cédex 05, France
Daniel Côme
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, tour 53, 1er étage, 4 place Jussieu, 75252 Paris cédex 05, France
*
*Fax: + 33 1 44 27 59 27 Email;[email protected]
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Abstract

Priming treatment of sunflower (Helianthus annuus L., cv Briosol) seeds for 7 days at 15°C with a polyethylene glycol solution at −2.0 MPa strongly improved their subsequent germination at 15°C on water. This stimulatory effect of priming remained after drying back the seeds at 20°C for 3 days. Malondialdehyde (MDA) and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were measured in control unprimed seeds, primed seeds, seeds primed then dried, and after 3 and 6 hours of imbibition of controland dried primed seeds in order to determine whether the cell antioxidant systems were involved in the beneficial effect of priming. The osmotreatment resulted in a strong increase in SOD and CAT activities but did not markedly affect MDA and GR activity. Following the 3 days of drying, MDA increased and the enzyme activities became similar to those measured in dry unprimed seeds, although the stimulatory effect of priming on germination remained. Imbibition of control dry seeds was associated with an increase in MDA and a decrease in CAT and GR activities, whereas reimbibition of dried primed seeds resulted in a decrease in MDA and an increase in SOD, CAT and GR activities. Isoform patterns on native gels showed no difference between treated (priming with or without subsequent drying) and control seeds for SOD (7 isoforms) and GR (5 isoforms), but the osmotreatment did induce a second isoform of CAT. The results obtained indicate that the CAT isoform pattern might be used as an indicato of the priming treatment that promotes germination. Involvement of theantioxidant systems in seed vigour is discussed

Keywords

catalasegerminationglutathione reductaseHelianthus annuuslipid peroxidationosmoprimingsunflower seedsuperoxide dismutase
Type
Research Article
Information
Seed Science Research , Volume 10 , Issue 1 , March 2000 , pp. 35 - 42
Copyright
Copyright © Cambridge University Press 2000

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