In 1926 and 1927, James W. Alexander and Kurt Reidemeister claimed to have made “the same” crucial breakthrough in a branch of modern topology which soon thereafter was called knot theory. A detailed comparison of the techniques and objects studied in these two roughly simultaneous episodes of mathematical research shows, however, that the two mathematicians worked in quite different mathematical traditions and that they drew on related, but distinctly different epistemic resources. These traditions and resources were local, not universal elements of mathematical culture. Even certain common features of the main publications such as their modernist, formal style of exposition can be explained by reference to particular constellations in the intellectual and professional environments of Alexander and Reidemeister. In order to analyze the role of such elements and constellations of mathematical research practice, a historiographical perspective is developed which emphasizes parallels with the recent historiography of experiment. In particular, a notion characterizing those “working units of scientific knowledge production” which Hans-Jörg Rheinberger has termed “experimental systems” in the case of empirical sciences proves helpful in understanding research episodes such as those bringing about modern knot theory.