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Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels

Published online by Cambridge University Press:  01 November 1999

RITA DAS  and
ROBIN REED
RITA DAS
Affiliation:
Harvard Medical School, Boston, Massachusetts 02115, USA
ROBIN REED
Affiliation:
Harvard Medical School, Boston, Massachusetts 02115, USA
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Abstract

A great deal of progress in elucidating the mechanisms of spliceosome assembly has been achieved by analyzing the A, B, and C spliceosomal complexes on native polyacrylamide gels. In contrast, progress in understanding the earliest spliceosomal complex E has been hampered because this complex dissociates on native gels and is difficult to detect by other methods. Here we report conditions for resolving the spliceosomal complex E using a native horizontal agarose mini-gel system. This system also provides a simple alternative to polyacrylamide gels for resolving the ATP-dependent spliceosomal complexes.

Keywords

E complexnative gelspliceosomespliceosome assemblysplicing
Type
METHODS
Information
RNA , Volume 5 , Issue 11 , November 1999 , pp. 1504 - 1508
Copyright
1999 RNA Society

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